Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

Nasim, Asma; Aslam, Bilal; Javed, Ijaz; Ali, Asghar; Muhammad, Faqir; Raza, Ahmad; Sindhu, Zia ud Din

doi:10.1002/jsfa.7220

Cited by 29 publications

(19 citation statements)

References 20 publications

(26 reference statements)

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“…Of these isolates, 46% were found resistant to commonly used antibiotics (Saleem et al, 2014). Similar incidence of AMROs in ready to eat raw food items has also been reported in other parts of the country (Shah et al, 2012;Capita and Alonso-Calleja 2013;Siddiqui et al, 2015Nasim et al, 2016.…”

Section: Development Of Amr and Its Impactsupporting

confidence: 82%

The Under Reported Issue of Antibiotic-Resistance in Food-Producing Animals in Pakistan

Rahman¹

2019

PVJ

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The recent revelation of a plasmid-mediated colistin resistant superbug isolated in China from food-producing animals (FPAs) and epidemic of a pan-drug resistant human typhoid strain in Hyderabad, Pakistan proved the notion that novel mechanisms of antibiotic resistance will emerge as we continue to rely on consistent use of antibiotics. The emergence and dissemination of antibiotic resistance is accelerated up by indiscriminate and incessant usage of antibiotics due to uncontrolled infections in hospital settings, community, FPAs and aquaculture. Unfortunately, a major part of the global antimicrobial usage is utilized in FPAs either for therapeutic purpose and/or as growth promoters raising vital concerns of the emergence of novel multidrug-resistant microorganisms. Pakistan-an agriculture-based country ranking in top ten livestock producing countries is currently shifting towards modern intense farming practices whereby animals are being supplemented with antibiotics on routine basis. However, unfortunately, monitoring usage of antibiotics in FPAs is lacking. Above all, the absence of systemic surveillance data on the level, frequency and impact of antimicrobial resistance further making it more challenging to devise antimicrobial-resistancecontrol strategies. We are highlighting this lack of information and hope that this review will help us in getting attention of policymakers for improving infection control measures and implementing laws on restricted use of antibiotics in animals.

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Section: Development Of Amr and Its Impactsupporting

confidence: 82%

The Under Reported Issue of Antibiotic-Resistance in Food-Producing Animals in Pakistan

Rahman¹

2019

PVJ

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“…All of meat samples have concentration of FF ranged between 106.1-707.28 μg/kg. The results of the present study can be confirmed by comparing with data from previous study (Nasim et al, 2016). According to current study, the residual concentration of FF is 164 much higher as compared to the previous study conducted by Zhang et al (2008).…”

Section: Discussionsupporting

confidence: 86%

Estimation of Florfenicol Residues in Layer Meat and Egg Samples using High Performance Liquid Chromatography

Fahim¹

2018

PVJ

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Antibiotics are widely used in the poultry industry to enhance the health and productivity of flocks which may have adverse effects on consumer's health. It is necessary to screen food products from animal origin for antimicrobial residues to safeguard the consumer's health. The present study was aimed to detect florfenicol (FF) residues in meat and egg samples of layer birds. For this purpose 150 meat and eggs samples were collected in equal ratio. High performance liquid chromatography (HPLC) was used to determine residual concentration of FF in meat and egg samples at wavelength of 223 nm. Ethyl acetate and phosphate buffer saline solution were used for extraction of FF from the samples. The mobile phase contained acetonitrile and water (27:73 v/v). Mean residual concentrations of FF as 61.56±13.19 and 281.08±57.46 µg/kg in meat and egg samples was detected. This study also showed that 80% (60) meat and 72% (54) egg samples were FF residue positive, out of these 86.7% (52) meat and 55.6% (30) egg samples were found to have residual concentrations above maximum residual limits. This contaminated meat may cause public health issues. There is a need to develop legislation about residual concentration of drugs in animal food products in Pakistan as well as to inform formers about the detrimental effects of drug residues on human health. To Cite This Article: FahimA, Aslam B, Mohsin M, Raza A, Faisal MN and Hussain A, 2018. Estimation of florfenicol residues in layer meat and egg samples using high performance liquid chromatography. Pak Vet J, 38(3): 329-332. http://dx.

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“…Various extraction methods were employed to extract CAP, TAP, FF, and FFA from animal-derived foods. These methods included LLE, 10,12,17,19,20 fabric-phase sorptive extraction (FPSE), 11 matrix solid-phase dispersion (MSPD), 15,22 LLE-solid-phase extraction (LLE-SPE), 16,24,25 QuEChERS extraction, 18 SPE, 21 and subcritical water extraction (SWE). 23 The LLE method for extracting samples is simple, but it consumes a large quantity of reagents and takes a long time and is thus not suitable for batch processing of samples.…”

Section: Selection Of Extraction Methodsmentioning

confidence: 99%

“…To date, various methods have been used to measure CAP, TAP, FF, and FFA in animal-derived foods; these methods include the fluorescent microsphere lateral flow immunoassay (FM-LFA), 7 enzyme-linked immunosorbent assay (ELISA), 8,9 reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UVD), 10 HPLC with diode array detection (DAD), 11 RP-HPLC with fluorescence detection (FLD), 12 LC-mass spectrometry (MS), 13,14 LC tandem mass spectrometry (MS/MS), [15][16][17][18][19][20] ultra-performance liquid chromatography (UPLC)-MS/ MS, [21][22][23][24] and gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). 25 In previous research, we developed an LC-MS/MS 20 method for the simultaneous determination of CAP, TAP, FF, and FFA in eggs.…”

mentioning

confidence: 99%

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

et al. 2019

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Accelerated solvent extraction was investigated as a novel alternative technology for the separation and quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine from poultry eggs, and the results were compared with the results of liquid–liquid extraction. Rapid quantification of the target compounds was carried out by ultra‐performance liquid chromatography‐electrospray ionization tandem triple quadrupole mass spectrometry. This optimized method was validated according to the requirements defined by the European Union and the United States Food and Drug Administration. Finally, the new approach was successfully applied to the quantitative determination of these analytes in 90 commercial poultry eggs from local supermarkets.

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Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

Cited by 29 publications

References 20 publications

The Under Reported Issue of Antibiotic-Resistance in Food-Producing Animals in Pakistan

The Under Reported Issue of Antibiotic-Resistance in Food-Producing Animals in Pakistan

Estimation of Florfenicol Residues in Layer Meat and Egg Samples using High Performance Liquid Chromatography

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

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