TILLING by sequencing (TbyS) reveals mutations in flowering control genes that are associated with altered plant architecture in Mungbean (Vigna radiata (L.) R. Wilczek)

Varadaraju, Anusheela; Ramadoss, Bharathi Raja; Gurunathan, Selvakumar; Balaram, Ramya; Ram, Sundaram Ganesh

doi:10.1007/s10722-020-01028-w

Cited by 7 publications

(4 citation statements)

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“…Additionally, unique haplotypes were identified and confirmed in a segregating population for PvTFL1y with either retrotransposon or splice-site mutation (Repinski et al, 2012), where a determinate type of growth was consistent with the earlier described recessive fin mutant in common bean (Kelly, 2001). Similar TFL1 homologs were found and reported in genotypes of hyacinth bean or Indian bean [Lablab purpureus (L.) Sweet] ( Kaldate et al, 2021) and among EMSinduced mutants of mung bean using a TILLING by sequencing approach (Varadaraju et al, 2021). In soybean, Dt1 orthologues was identified as GmTFL1 (Glyma19g37890), located on chromosome 19 with two dominant and four recessive alleles.…”

Section: -29supporting

confidence: 80%

“…In contrast to the previous candidate, the WD40 gene was moderately expressed in all tissues including roots, nodules, leaves, stems, and pods, but not in flower buds and flowers 3 . WD40 proteins contain a typical Trp-Asp motif with highly conserved repeating units (Neer et al, 1994), and they play a regulatory role in a diverse range of functions in plants including meristem and floral development, light signalling, cell division, cytokinesis, and apoptosis (van Nocker and Ludwig, 2003). A phosphatase protein with several WD40 repeats interacts with protein kinase (SnRK1) and regulates Arabidopsis plant growth in conditions of different nutrient availabilities (Ananieva et al, 2008), but it was not studied in legumes.…”

Section: Wd40 Gene (Wd40-repeat Protein)mentioning

confidence: 99%

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Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops

et al. 2022

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Height from soil at the base of plant to the first pod (HFP) is an important trait for mechanical harvesting of legume crops. To minimise the loss of pods, the HFP must be higher than that of the blades of most combine harvesters. Here, we review the genetic control, morphology, and variability of HFP in legumes and attempt to unravel the diverse terminology for this trait in the literature. HFP is directly related to node number and internode length but through different mechanisms. The phenotypic diversity and heritability of HFP and their correlations with plant height are very high among studied legumes. Only a few publications describe a QTL analysis where candidate genes for HFP with confirmed gene expression have been mapped. They include major QTLs with eight candidate genes for HFP, which are involved in auxin transport and signal transduction in soybean [Glycine max (L.) Merr.] as well as MADS box gene SOC1 in Medicago trancatula, and BEBT or WD40 genes located nearby in the mapped QTL in common bean (Phaseolus vulgaris L.). There is no information available about simple and efficient markers associated with HFP, which can be used for marker-assisted selection for this trait in practical breeding, which is still required in the nearest future. To our best knowledge, this is the first review to focus on this significant challenge in legume-based cropping systems. KEYWORDS auxin transport and signal transduction genes, BEBT or WD40 genes, candidate genes, gene expression, height to the first pod, MADS box gene SOC1, QTL analysis Frontiers in Plant Science 01 frontiersin.org Kuzbakova et al. 10.3389/fpls.2022.948099 In contrast, as shown in the bottom part of Table 1, many reports in the literature use the same or similar terms for 'pods, ' where FPH (first pod height) is clearly the most commonly used, while the terms for 'bottom, ' 'lowest, ' and 'basal' pods are synonymous. Interestingly, terms referring to 'pod insertion' were used by almost all researchers from Brazil (with one Frontiers in Plant Science 03 frontiersin.org

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Section: -29supporting

confidence: 80%

Section: Wd40 Gene (Wd40-repeat Protein)mentioning

confidence: 99%

Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops

et al. 2022

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“…In chickpea, TILLING was also used to diagnose mutations in the M2 generation. Recently, in mungbean, five exon residing mutations were identified by TILLING and confirmed the potential role of each mutation in altering mungbean plant architecture to develop an ideal plant type [58].…”

Section: Eco Tillingmentioning

confidence: 81%

Recent Advancements in Genetic Improvement of Food Legume Crops

Punia¹,

Rolaniya²,

Jat³

2023

Case Studies of Breeding Strategies in Major Plant Species

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Legumes are the second-largest source of food after cereals, all over the world, and an essential protein source in the vegetarian diet. These crops remain essential to subsistence production as they have the inherent capacity to survive in an adverse ecosystem and require minimum investment for their management. The increasing challenge of feeding a rapidly growing population places excessive pressure on current food production systems, which can no longer be sustained by traditional plant breeding alone. Therefore, modern breeding methods with increased genetic gains are required to meet the food demand of the increasing population. In the past few decades, the efficiency of legume crop breeding programs has increased considerably using novel and multidisciplinary approaches in breeding programs. A multidisciplinary approach combining conventional plant breeding, mutation breeding, plant biotechnology, and molecular breeding is strategically ideal for production of new and improved crop varieties. This chapter focuses on recent advancements in plant biotechnology, related molecular methods, phenomics, and their application in breeding of legume crops.

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“…This approach is known as TILLING-by-Sequencing+ (TbyS+), which has been used to identify mutations in genes controlling stress resistance in peanut (Guo et al 2015) and fatty acid biosynthesis pathway in soybean (Guo et al 2015;Lakhssassi et al 2021). Similarly, TbyS has been used to identify the role of GIGANTEA (GI), RAMOSUS (RMS), and TERMINAL FLOWER1 (TFL1) genes controlling flowering and therefore alter plant architecture of mungbean (Varadaraju et al 2021). TILLING technology holds new prospects to clone genes for disease resistance and abiotic stress tolerance in cereal crops under current scenario of climate change (Bettgenhaeuser and Krattinge 2019).…”

Section: An Overview Of Reverse Genetic Approachesmentioning

confidence: 99%

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

Kumar

Gupta

et al. 2022

Heredity

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In the last decade, advancements in genomics tools and techniques have led to the discovery of many genes. Most of these genes still need to be characterized for their associated function and therefore, such genes remain underutilized for breeding the next generation of improved crop varieties. The recent developments in different reverse genetic approaches have made it possible to identify the function of genes controlling nutritional, biochemical, and metabolic traits imparting drought, heat, cold, salinity tolerance as well as diseases and insect-pests. This article focuses on reviewing the current status and prospects of using reverse genetic approaches to breed nutrient-rich and climate resilient cereal and food legume crops.

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TILLING by sequencing (TbyS) reveals mutations in flowering control genes that are associated with altered plant architecture in Mungbean (Vigna radiata (L.) R. Wilczek)

Cited by 7 publications

References 58 publications

Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops

Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops

Recent Advancements in Genetic Improvement of Food Legume Crops

Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops

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