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Interpretation :Studying genetic diversity is useful for wheat breeding and production of more efficient wheat cultivars under changing climatic conditions. Forty-four diverse bread wheat genotypes were assessed for genetic diversity study using 70 microsatellite (SSRs) markers covering all three genomes.Genomic DNA of 44 genotypes was extracted using CTAB method and quality was checked by UV spectrophotometer and agarose gel electrophoresis. PCR amplification reaction was carried out and amplified products were resolved by horizontal electrophoresis. Bands were scored and matrix was used to calculate the similarity genetic distance. Phenotypic data was subjected to Euclidean cluster analysis for estimation of genetic divergence and grouping of genotypes into clusters.
A set of eighty one genotypes comprising sixty three newly developed single cross quality protein maize hybrids produced by 9 lines x 7 testers crosses along with sixteen parental inbred lines and two standard checks were evaluated for genetic variability, broad sense heritability and genetic advance at CCS, Haryana Agricultural University, Regional Research Station, Karnal, during kharif 2014. Highly significant values of mean sum of squares due to genotypes for all the 17characters studied could be recorded. Close correspondence between GCV and PCV was observed for all the traits except number of cobs per plant and cob diameter. The characters viz., number of grains per cob, tryptophan content, lysine content and grain yield per plant were recorded high estimates of heritability and genetic advance as per cent of mean indicating them to be controlled by additive genes.
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