Microsatellite markers based characterization in advance breeding lines and cultivars of bread wheat

Phougat, Divya; Panwar, I. S.; Punia, M. S.; Sethi, S. K.

doi:10.22438/jeb/39/3/mrn-607

Cited by 9 publications

(8 citation statements)

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“…The average number of fragments was 2.0 per marker with number of amplified bands ranging from 1 to 4. Similar results were also reported in previous studies (Drikvand et al 2015 andPhougat et al 2018). Banding pattern of bread wheat parents and their F 1 s using primer xbarc 220 was observed.…”

Section: Resultssupporting

confidence: 91%

Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)

Phougat

Sethi

2020

Indian J Agri Sci

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Malnutrition leads to high mortality especially in developing countries. Present study comprised 11 high yielding wheat (Triticum aestivum L.) genotypes sown during rabi 2014-15 and 2015-16 and crosses attempted during 2014-15 and sown at Research Field of CCS Haryana Agricultural University, Hisar, Haryana. Forty nine microsatellite markers linked with identified QTLs for micronutrient were used for the genetic diversity assessment among bread wheat parents their F1s. Out of 49, 38 SSRs were found polymorphic which amplified a total of 76 alleles with an average of 2.00 alleles per locus. The range of amplified PCR products ranged from 100 to 480 bp. Estimates of similarity coefficient among parents and F1s suggest enough divergence and averaged to 0.71. Enough genetic variability was observed for all the 14 agronomic traits in all genotypes. Selection of genotypes from different clusters helps in making proper choice of breeding programme. In the present study, the information generated about genetic diversity will be useful for selection of parents for breeding including gene mapping and ultimately for marker assisted selection (MAS) for micronutrient content improvement worldwide.

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Section: Resultssupporting

confidence: 91%

Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)

Phougat

Sethi

2020

Indian J Agri Sci

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“…The variability in the number of alleles per locus may results from different locus-specific mutation rates and reflects strong differences in allelic diversity between SSRs loci (Piyusha and Singh, 2018). The mean number of alleles estimated in the three wheat genotypes (2.62 alleles per locus) is consistent with a study conducted by Phougat et al (2018) that found an average of 2.585 alleles per locus in 44 bread wheat genotypes using 100 SSR markers. Moreover, higher allele means numbers (5.9 and 13) were detected in 10 and 40 wheat genotypes by Ateş Sönmezoğlu and Terzi (2017) and Bousba et al…”

Section: Results and Discussion I-ssr Marker Informative And Genetic Relationshipssupporting

confidence: 89%

“…In addition to Quarrie et al (2005) that the D genome gave the lowest number of polymorphic markers in hexaploid wheat maps, although the map length of the D genome was similar to those of the A and B genomes. The results of the present study along with other studies discussed above clearly demonstrate the utility of microsatellite markers in fast and high throughput fingerprinting of numbers of genotypes for detecting polymorphism and estimation of genetic diversity (Han et al, 2015;Karima et al, 2017;Kumar et al, 2016;Phougat et al, 2018). On other hand, chromosome 7 is conserving the most important chromosome harbinger QTL for drought (Morgan and Tan, 1996).…”

Section: Results and Discussion I-ssr Marker Informative And Genetic Relationshipssupporting

confidence: 79%

Validation of SSR Molecular Markers Linked to Drought Tolerant in Some Wheat Cultivars

Eid

2018

J. Plant Breed. Genet.

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The present study was carried out to conduct drought tolerance in three wheat cultivars including susceptible (Gemmiza7) and tolerant (Sakha93 and Sahel1). Molecular characterization was done by 26 SSR markers located on chromosome7 which was associated with drought tolerance in many previous studies. 26 SSR markers were polymorphic and thus showed 100% polymorphism. The number of alleles per locus varied from 2 to 3 alleles with an average (2.62). The polymorphism information content (PIC) value ranged from 0.34 to 0.59, with a mean of 0.51. The discrimination power (Dp) value ranged between 0.67 and 0.78 with an average of 0.71 per locus and Heterozygosity (He) value varied from 0.44 to 0.67 with an average of 0.59. The genetic relationships estimated by the polymorphism of SSR markers revealed a greater level of genetic variability in wheat cultivars of wide adaptability and applicability. Whereas an average of combined probability value for the SSR markers was 6.15 x 10-16, suggests the capability of the marker system to distinguish identity and purity of wheat cultivars. In addition to the SSR markers revealed various bands that were either absent or present within tolerant cultivars (Sakha93 and Sahel1) which were altogether absent in susceptible cultivar (Gemmiza7). Also, SSRs of diagnostic and curatorial importance were discerned as ‘stand-alone’ molecular descriptors for barcoding the application of DNA sequences of standardized genetic markers for the identification of wheat cultivars. However, the genetic information in this study could provide useful information to address breeding programs and germplasm resource management.

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“…From their development, their use in agriculture increased through the construction of genetic maps in crop species, the association between molecular markers and important agronomic traits, the dissection of quantitative traits, and the positional cloning of genes of interest. Besides the estimation of genetic distances and molecular cloning, molecular markers provide the most suitable tool for the evaluation of genetic diversity, allowing for the selection of the most suitable parental lines in breeding programs, the management of germplasm collections, and varietal identification [2].…”

Section: Introductionmentioning

confidence: 99%

Molecular Marker Technology for Crop Improvement

Soriano

2020

Agronomy

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Since the 1980s, agriculture and plant breeding have changed with the development of molecular marker technology. In recent decades, different types of molecular markers have been used for different purposes: mapping, marker-assisted selection, characterization of genetic resources, etc. These have produced effective genotyping, but the results have been costly and time-consuming, due to the small number of markers that could be tested simultaneously. Recent advances in molecular marker technologies such as the development of high-throughput genotyping platforms, genotyping by sequencing, and the release of the genome sequences of major crop plants open new possibilities for advancing crop improvement. This Special Issue collects sixteen research studies, including the application of molecular markers in eleven crop species, from the generation of linkage maps and diversity studies to the application of marker-assisted selection and genomic prediction.

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Microsatellite markers based characterization in advance breeding lines and cultivars of bread wheat

Cited by 9 publications

References 0 publications

Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)

Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)

Validation of SSR Molecular Markers Linked to Drought Tolerant in Some Wheat Cultivars

Molecular Marker Technology for Crop Improvement

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