Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

Wang, Tao; Chen, Changying; Larcher, Leon M; Barrero, Roberto A.; Veedu, Rakesh N.

doi:10.1016/j.biotechadv.2018.11.001

Cited by 370 publications

(281 citation statements)

References 182 publications

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“…Aptamers are smaller and easier to prepare and modify than monoclonal antibodies, they penetrate tissue better and they show lower toxicity and immunogenicity. Therefore, aptamers are widely used for tumor detection and targeted chemotherapy [11][12][13]. The SELEX method has been used to identify an aptamer, Sgc8 that binds specifically to CEM human acute T lymphocyte leukemia cells [14,15].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Cell-Targeting Drug Delivery System Based on Aptamer-Modified Mesoporous Silica Nanoparticles

et al. 2019

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How to deliver chemotherapeutic drugs efficiently and selectively to tumor cells to improve therapeutic efficacy remains a difficult problem. We herein construct an efficient cell-targeting drug delivery system (Sgc8-MSN/Dox) based on aptamer-modified mesoporous silica nanoparticles that relies on the tumor-targeting ability of the aptamer Sgc8 to deliver doxorubicin (Dox) to leukemia cells in a targeted way, thereby improving therapeutic efficacy and reducing toxicity. In this work, Sgc8-MSN/Dox showed sustained Dox release, and they targeted and efficiently killed CCRF-CEM human acute T lymphocyte leukemia cells, suggesting potential as a cancer therapy.

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Section: Introductionmentioning

confidence: 99%

An Efficient Cell-Targeting Drug Delivery System Based on Aptamer-Modified Mesoporous Silica Nanoparticles

et al. 2019

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“…There is a combination of issues, impeding the widespread application of aptamers in the control of antimicrobial residues in foodstuffs. The reasons could be both technical (i.e., difficulty of aptamer production for small molecules, sometimes insufficient target-aptamer affinity, lack of standardized protocols) [52,53] and economical (i.e., small veterinary market, diagnostics market dominated by antibody-based assays (immunoassays) for decades, industries reluctant to invest capital in new developments to replace antibodies) [54]. Many published applications are for single analyte detection.…”

Section: Discussionmentioning

confidence: 99%

“…The reasons for the lack of aptamers available could be both technical and economical. Even though many improvements occurred in the aptamer production since 30 years, the selected aptamers sometimes do not have the desired affinity and specificity [52]. Progresses in SELEX procedures to produce performing aptamers with high binding capacity and specificity are always needed, especially for small molecules.…”

Section: Aptamers' Characteristics: Advantages and Drawbacksmentioning

confidence: 99%

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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The presence of antimicrobial residues in food-producing animals can lead to harmful effects on the consumer (e.g., allergies, antimicrobial resistance, toxicological effects) and cause issues in food transformation (i.e., cheese, yogurts production). Therefore, to control antimicrobial residues in food products of animal origin, screening methods are of utmost importance. Microbiological and immunological methods (e.g., ELISA, dipsticks) are conventional screening methods. Biosensors are an innovative solution for the development of more performant screening methods. Among the different kinds of biosensing elements (e.g., antibodies, aptamers, molecularly imprinted polymers (MIP), enzymes), aptamers for targeting antimicrobial residues are in continuous development since 2000. Therefore, this review has highlighted recent advances in the development of aptasensors, which present multiple advantages over immunosensors. Most of the aptasensors described in the literature for the detection of antimicrobial residues in animal-derived food products are either optical or electrochemical sensors. In this review, I have focused on optical aptasensors and showed how nanotechnologies (nanomaterials, micro/nanofluidics, and signal amplification techniques) largely contribute to the improvement of their performance (sensitivity, specificity, miniaturization, portability). Finally, I have explored different techniques to develop multiplex screening methods. Multiplex screening methods are necessary for the wide spectrum detection of antimicrobials authorized for animal treatment (i.e., having maximum residue limits).

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“…Aptamers are functional single-stranded DNA (ssDNA) or RNA sequence that fold into complex tertiary structures and bind to specic molecular targets in a manner similar to that of antibodies. 23,24 Thus, aptamers are called "chemical antibodies". 25 The vast majority of aptamers are obtained from the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method in vitro.…”

Section: Introductionmentioning

confidence: 99%

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

Zhou

et al. 2020

RSC Adv.

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Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

Cited by 370 publications

References 182 publications

An Efficient Cell-Targeting Drug Delivery System Based on Aptamer-Modified Mesoporous Silica Nanoparticles

An Efficient Cell-Targeting Drug Delivery System Based on Aptamer-Modified Mesoporous Silica Nanoparticles

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

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