Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

Li, Zhen; Hu, Bo; Zhou, Rong; Zhang, Xiaojuan; Wang, Ruizhe; Gao, Yun Tao; Sun, Mingjuan; Jiao, Binghua; Wang, Lianghua

doi:10.1039/c9ra10600f

Cited by 19 publications

(5 citation statements)

References 65 publications

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“…Therefore, aptasensors have demonstrated their useful applications for the real-time monitoring of LMW molecules such as pollutants, recreational drugs, and toxins in complex media. However, examples of BLI aptasensors for the direct detection of LMW analytes are seldom reported and are focused on marine toxins − with molecular weights >411 Da …”

mentioning

confidence: 99%

Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry

et al. 2020

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The direct biolayer interferometry (BLI) measurement of lowmolecular-weight (LMW) analytes (<200 Da) still represents a challenge, in particular, when low receptor densities are used. BLI is a powerful optical technique for the label-free, real-time characterization and quantification of biomolecular interactions at interfaces. We demonstrate herein that the quantification of biomolecular recognition is possible by BLI using either 2Dlike or 3D platforms for aptamer ligand immobilization. The influence of the aptamer density on the interaction was evaluated and compared for the two sensor architectures. Despite the LMW of the analyte, BLI monitoring led to signals that are exploitable for affinity and kinetic studies, even at low aptamer density. We demonstrate that the immobilization format as well as the aptamer density has a crucial influence on the determination of the recognition parameters.

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mentioning

confidence: 99%

Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry

et al. 2020

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“…It has been reported that the affinity of several aptamers was optimized by sequence truncation [ 58 , 69 , 70 ]. Therefore, we generated some truncated forms of G48 and measured the affinity of them by BLI ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Selection, Characterization, and Optimization of DNA Aptamers against Challenging Marine Biotoxin Gymnodimine-A for Biosensing Application

Zhang

Gao²,

Deng³

et al. 2022

Toxins

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Gymnodimines (GYMs), belonging to cyclic imines (CIs), are characterized as fast-acting toxins, and may pose potential risks to human health and the aquaculture industry through the contamination of sea food. The existing detection methods of GYMs have certain defects in practice, such as ethical problems or the requirement of complicated equipment. As novel molecular recognition elements, aptamers have been applied in many areas, including the detection of marine biotoxins. However, GYMs are liposoluble molecules with low molecular weight and limited numbers of chemical groups, which are considered as “challenging” targets for aptamers selection. In this study, Capture-SELEX was used as the main strategy in screening aptamers targeting gymnodimine-A (GYM-A), and an aptamer named G48nop, with the highest KD value of 95.30 nM, was successfully obtained by screening and optimization. G48nop showed high specificity towards GYM-A. Based on this, a novel aptasensor based on biolayer interferometry (BLI) technology was established in detecting GYM-A. This aptasensor showed a detection range from 55 to 1400 nM (linear range from 55 to 875 nM) and a limit of detection (LOD) of 6.21 nM. Spiking experiments in real samples indicated the recovery rate of this aptasensor, ranging from 96.65% to 109.67%. This is the first study to report an aptamer with high affinity and specificity for the challenging marine biotoxin GYM-A, and the new established aptasensor may be used as a reliable and efficient tool for the detection and monitoring of GYMs in the future.

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“…The wavelength shift is directly related to the distance between the two reflecting surfaces (i.e., it correlates with changes in the thickness of the biolayer resulting from analyte detection) [ 120 ]. This technique has been successfully used for the detection of domoic acid [ 120 ], palytoxin [ 123 ], saxitoxin [ 124 ], and dinophysistoxin-1 [ 119 ], with low LODs. In the case of palytoxin detection, the signal was amplified by HRP-labeled aptamers specific for palytoxin immobilized on the biosensor surface.…”

Section: Optical Biosensors For Detecting Aquatic Biotoxinsmentioning

confidence: 99%

Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

Vaz

Valpradinhos

Frasco

et al. 2021

Sensors

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Optical biosensors are used in numerous applications and analytical fields. Advances in these sensor platforms offer high sensitivity, selectivity, miniaturization, and real-time analysis, among many other advantages. Research into bioactive natural products serves both to protect against potentially dangerous toxic compounds and to promote pharmacological innovation in drug discovery, as these compounds have unique chemical compositions that may be characterized by greater safety and efficacy. However, conventional methods for detecting these biomolecules have drawbacks, as they are time-consuming and expensive. As an alternative, optical biosensors offer a faster, simpler, and less expensive means of detecting various biomolecules of clinical interest. In this review, an overview of recent developments in optical biosensors for the detection and monitoring of aquatic biotoxins to prevent public health risks is first provided. In addition, the advantages and applicability of these biosensors in the field of drug discovery, including high-throughput screening, are discussed. The contribution of the investigated technological advances in the timely and sensitive detection of biotoxins while deciphering the pathways to discover bioactive compounds with great health-promoting prospects is envisaged to meet the increasing demands of healthcare systems.

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Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

Abstract: For the first time, the aptamer of dinophysistoxin-1 was successfully obtained with high affinity and specificity by SELEX, and an aptasensor with a detection range from 40 to 600 nM was developed by biolayer interferometry.

Cited by 19 publications

References 65 publications

Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry

Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry

Selection, Characterization, and Optimization of DNA Aptamers against Challenging Marine Biotoxin Gymnodimine-A for Biosensing Application

Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

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