The Usefulness of RAPD and AFLP Markers for Determining Genetic Similarity in Rye (Secale L.) Species and Subspecies

Ćwiklińska, Anna; Broda, Z.; Bocianowski, Jan; Dobrzycka, Agnieszka

doi:10.2478/v10182-010-0003-y

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…Lefort-Buson et al (1987) and Ali et al (1995) observed in B. napus, andFalk et al (1994) in B. rapa, that using morphological markers and geographic origin caused an increase in heterosis with increasing parental distance. Moreover, in many crops molecular DNA markers have proven to be an excellent tool for the assessment of genetic relationships to monitor genetic diversity or similarity and relatedness among breeding material (Stępień et al, 2007;Chen et al, 2008;Ćwiklińska et al, 2010;Bus et al, 2011). Relationships between the genetic distance or similarity of parental lines of F 1 hybrids with yielding ability and heterosis effect have been examined intensively for different crops, such as maize (Zea mays L.), rice (Oryza sativa L.), wheat (Triticum aestivum L.), and oilseed rape (Zhao et al, 1999;Corbellini et al, 2002;Schrag et al, 2009;Girke et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Liersch

Bocianowski

Wos³

et al. 2016

Crop Science

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The level of genetic diversity or similarity between parents has been proposed as a predictor of F1 generation performance and heterosis for seed yield. The objective of this study was to determine the feasibility of using amplified fragment length polymorphism (AFLP) fluorescent‐labeled primers to evaluate the genetic relationships between 101 oilseed rape (Brassica napus L.) genotypes to maximize the selection of different parents in a breeding program. The collection of genotypes included 25 cytoplasmic male sterility ogura and 21 restorer lines (Rfo). The set of genotypes was completed with B. napus cultivars of Polish, European, and Asian origin and resynthesized lines. Ten primer‐enzyme combinations (EcoRI and MseI) were used. Genetic similarities among genotypes were estimated in accordance with the Nei and Li coefficient of similarity, and then the unweighted pair group method using arithmetic averages method was applied for the creation of a dendrogram. A total of 344 reproducible, polymorphic markers were obtained. Unweighted pair group method using arithmetic averages clustering based on the Nei and Li coefficient of similarity separated the 101 genotypes into four distinct clusters. Analysis of molecular variance showed that 90% of the detected variation was found within the group, while the variation among groups contributed to only 10%. Our results indicate that the AFLP technique seemed to be a suitable molecular method for assessing genetic relationships between oilseed rape inbred lines with high levels of accuracy. Genetic similarity evaluated on the basis of AFLP molecular markers is a significant addition to classical methods of selection of parental components of winter oilseed rape hybrid varieties (e.g., ‘Poznaniak’).

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Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Liersch

Bocianowski

Wos³

et al. 2016

Crop Science

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“…primer hybridization sites, screening methods, i.e. RAPD (Ćwiklińska et al, 2010; and c -groups of similarity 1 -Ot0-6; 2 -bulk -RILs tolerant to nutrient deprivation; 3 -F 1 (Ot0-6 × Ot1-3); 4 -bulkRILs susceptible to nutrient deprivation; 5 -Ot1-3 Fig. 4.…”

Section: Discussionmentioning

confidence: 99%

“…Amplification is performed within new microsatellite and inter-microsatellite DNA regions, which are not amplified separately using RAPD (Ćwiklińska et al, 2010;Myśków et al, 2001) or ISSR (Bolibok et al, 2006;Ye et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

R-ISSR - Tool for Generation of a New Type of Products, Applied for the Identification of Putative Molecular Marker Linked to QTL Determined Tolerance to Nutrient Deprivation Stress in Rye (Secale cereale L.)

Smolik

2012

Not Bot Hort Agrobot Cluj

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This study presents results confirming the possibility of the application of various combinations of RAPD and ISSR primers in one multiplex PCR for the rye genome and generating a new type of R-ISSR products. The plant material consisted of two inbred lines (Ot0-6 and Ot1-3), F 1 and two bulks (tolerant and susceptible), selected from a population of RILs (F 9 ) with different responses to stress caused by nutrient deprivation at the seedling stage. In one PCR reaction, the DNA of five genotypes was amplified separately, with RAPD, ISSR, and RAPD+ISSR primers. In total, 28 R-ISSR combinations were tested by combining 20 and 8 appropriately selected RAPD and ISSR primers. 567 loci were amplified, including 230 RAPD, 136 ISSR and 207 amplified R-ISSR loci, of which 114 were new. It was shown that only one ISSR product was identified amongst sequenced R-ISSR products with identical electrophoretic mobility as comigrating RAPDs and ISSRs. The remaining ones had heteroamplicons (R-ISSR) sequences. A similar range of variability was observed both in R-ISSR profiles and in RAPD, as well as in ISSR. The correlation coefficient between the matrices of genetic similarity for five rye genotypes calculated by means of the Mantel test was highly significant r AB.C = 0.964.

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“…Isozymes, RAPD and AFLP markers have been used to estimate genetic diversity/similarity in various crop species (Foisset et al, 1996;Corbellini et al, 2002) and species of the genus Brassica (Snowdon and Friedt, 2004;Hu et al, 2007). The RAPD method is one of the simplest and cheapest molecular techniques; RAPD markers have been used successfully to analyze genetic diversity, identify, quantify and characterize genetic variation in all available germplasm resources (Ćwiklińska et al, 2010;Bocianowski and Seidler-Łożykowska, 2012). The AFLP method has been applied in research involving, for example, identification of sequence variability (Vos et al, 1995) and prediction of hybrid performance in maize using AFLP and SSR molecular markers (Schrag et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Isozyme, RAPD and AFLP Markers in Genetic Similarity Assessment of CMS Ogura F1 Hybrids of Winter Oilseed Rape (Brassica Napus L.) Parental Lines

Liersch¹,

Bocianowski

Kozak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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Isozyme, RAPD and AFLP markers were evaluated and compared for their ability to determine genetic similarity in a set of 18 parental lines of winter oilseed rape F 1 hybrids developed using CMS ogura. Five isozyme systems, 64 RAPD starters and 23 EcoRI+3/MseI+3 AFLP primer combinations generated 597 polymorphic markers. These polymorphic fragments were chosen for estimation of genetic similarity. Of the total number of polymorphic products, polymorphic zymograms constituted only 3.0% of the markers, 57 RAPD primers 37.7%, and 23 AFLP primer combinations 59.3%. The size of RAPD polymorphic products ranged from 564 to 2100 bp. On average there were four amplified bands per primer, with 61.0% polymorphism. The AFLP polymorphic fragments ranged from 72 to 1352 bp in size. AFLP assays generated 15 bands per primer pair on average and detected roughly four times more bands than with RAPD analysis. The genetic similarity coefficients based on all marker data range from 0.52 to 0.84. The correlation of genetic similarities based on RAPD and AFLP markers was 0.58. Estimated genetic similarity based on isozyme data was not correlated with genetic similarity derived from the two DNA-based markers. The dendrogram constructed with the three types of markers taken together grouped all the winter oilseed rape parental lines into several similar clusters. The genomic distribution and frequency of the RAPD and AFLP markers can serve well as estimators of genetic similarity between parental lines of F 1 CMS ogura hybrids.K Ke ey y w wo or rd ds s: : Winter oilseed rape, Brassica napus, F 1 hybrids CMS ogura, genetic similarity, molecular markers.

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The Usefulness of RAPD and AFLP Markers for Determining Genetic Similarity in Rye (Secale L.) Species and Subspecies

Cited by 10 publications

References 18 publications

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

R-ISSR - Tool for Generation of a New Type of Products, Applied for the Identification of Putative Molecular Marker Linked to QTL Determined Tolerance to Nutrient Deprivation Stress in Rye (Secale cereale L.)

Comparison of Isozyme, RAPD and AFLP Markers in Genetic Similarity Assessment of CMS Ogura F1 Hybrids of Winter Oilseed Rape (Brassica Napus L.) Parental Lines

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