The use of polyvinyl alcohol glutaraldehyde antigen coated discs for laser induced fluorescence detection of plague

Araújo, Adriana Mello de; Almeida, Alzira Maria Paiva de; Azevêdo, W.M. de

doi:10.1016/s0925-4005(97)80109-8

Cited by 15 publications

(11 citation statements)

References 7 publications

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“…In our laboratories, several supports have been proposed for biomolecules immobilization: Dacron [42,43]; ferromagnetic Dacron [44,45]; polyvinyl alcohol-glutaraldehyde network [46][47][48]; polyaniline-Dacron composite [49]; plasticized filter paper with polyvinyl alcohol-glutaraldehyde [50].…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

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This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane-polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.

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Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

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“…Due to its biocompatability, PVA could also be used in a variety of biomedical applications. 3,10,11 A PVA gel membrane could be used as a synthetic membrane for pervaporation and as a bioartificial pancreas in biomedical applications. 4 -11 The reaction between PVA and glutaraldehyde (GA) generates a polymer network, whose mechanical properties and swelling are affected by its degree of crosslinking.…”

Section: Introductionmentioning

confidence: 99%

On the viscoelastic properties of poly(vinyl alcohol) and chemically crosslinked poly(vinyl alcohol)

Park

Ruckenstein

2001

J of Applied Polymer Sci

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Poly(vinyl alcohol)(PVA) films chemically crosslinked with glutaraldehyde(GA) in the presence of HCl were prepared by casting from aqueous solutions. The PVA and PVA gels were investigated by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and dynamic mechanical analysis (DMA); their swelling characteristics and tensile strength were also determined. The DSC results for the gels displayed depressions of the melting and crystallization temperatures, as well as a decrease of the heat of fusion, when compared to those of PVA free of crosslinker. The DMA analysis revealed that: (1) The glass transition temperature of the wet PVA was lower than that of the dry one, indicating that the water had a plasticizing effect.(2) The gels had a lower glass transition temperature than PVA. (3) The glass transition temperature of the wet gels increased with increasing crosslink density. Possible explanations are provided for these observations. Whereas the thermogravimetric curves of PVA exhibited a single degradation peak, two degradation peaks were detected for the crosslinked PVA. The wet PVA and PVA gels displayed lower tensile strengths and higher elongations than the dried ones.

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“…In our laboratories other procedures for antigen covalent immobilization on supports have been proposed such as Dacron (11), ferromagnetic Dacron (12,13), interpenetrated network of PVA-glutaraldehyde (14)(15)(16)(17), filter paper plasticized with PVA-glutaraldehyde (18), and a polyaniline-Dacron composite (19). The optimal values of F1 antigen amount, conjugate dilution and serum titer established for the present method (1.25 µg/disc; 1:4,000 and 3,200, respectively) can be compared to those found for PVA-glutaraldehyde (1.3 µg/disc; 1:6,000 and 1:12,600), plasticized filter paper (1.25 µg/disc; 1:12,000 and 1:6,400), and polyaniline-Dacron (2 µg/disc; 1:4,000 and 1:800).…”

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confidence: 99%

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

Barros

Almeida

Carvalho

et al. 2002

Braz J Med Biol Res

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We developed an efficient method to prepare a hybrid inorganicorganic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates, with the amount of antigen being one fourth that employed in conventional PVC plates (5 µg/well). In addition to the performance of the polysiloxane/PVA-glutaraldehyde disc as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.

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The use of polyvinyl alcohol glutaraldehyde antigen coated discs for laser induced fluorescence detection of plague

Cited by 15 publications

References 7 publications

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

On the viscoelastic properties of poly(vinyl alcohol) and chemically crosslinked poly(vinyl alcohol)

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

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