The Use of IgE Mediated Responses as a Pharmacologic Test System. The Effect of Disodium Cromoglycate in Respiratory and Cutaneous Reactions and on the Electrocardiograms of Rhesus Monkeys

Abstract

Disodium cromoglycate (DSCG) was used in a controlled laboratory study of an IgE-mediated, immediate-type, respiratory response in primates. The respiratory response to antigen was partially inhibited by DSCG. Systemic administration of DSCG did not inhibit active cutaneous reactivity to ascaris antigen or reverse passive cutaneous reactivity to anti-human IgE in rhesus monkeys, DSCG did not inhibit the respiratory eosinophil response which occurs after ascaris challenge in a highly reactive monkey. A possible explanation for the dissociation of the cutaneous and respiratory activity of DSCG in primate immediate-type responses may be the existence of more than one population of mast cells. The intravenous administration of DSCG resulted in transient aberrantly conducted premature beats in 3 normal rhesus monkeys.

A Comparative Study of the Anti-Allergic Effects of Disodium Baicalein 6-Phosphate (BPS) and Disodium Cromoglycate (DSCG)

“…Thus, both drugs appear to have a similar affinity to the mast cell of monkey lung and human reagin. Patterson et al (24) suggested that there may be differences in the population of mast cells between the respiratory organs and the skin, since DSCG does not show an inhibition in the mediator release form monkey skin sensitized with human reagin. Therefore, the reactivity of mast approximately 4.80 A between the two chromone-nuclei, while the site of the non-reaginic antibody, IgG, is shorter or longer than that of reaginic antibody, and is readily fixed with the two molecules of baicalein or BPS and shown in Fig.…”

Reagin-mediated asthma in rhesus monkeys and relation to bronchial cell histamine release and airway reactivity to carbocholine.

“…G50 and 75 column fraction of Ascaris suum extract prepared by the method of Hogarth-Scott (2). Standardization of each preparation of AA was done both by determination of protein as estimated by optical density at 280 nm (Beckman DU spectrophotometer; Beckman Instruments, Fullerton, Calif.) and cutaneous titration in AA-sensitive animals (3). The preparation of AA in current use contains 0.8 mg protein/ml and is stored at 4"C in phosphate-buffered 0.15 M NaCl, pH 7.35 (PBS).…”

Ionophore and Arachidonic Acid Stimulation of Airway Responses in Rhesus Monkeys

“…Base-line pulmonary function studies were obtained. Next the test aerosol challenge was delivered in a standard manner (7). All challenges were with an in-line nebulizer in a Bird Mark 7 (Bird Corp., Palm Springs, Calif.) respirator with all settings controlling respirations constant for each experiment in each animal.…”