Reagin-mediated asthma in rhesus monkeys and relation to bronchial cell histamine release and airway reactivity to carbocholine.

The in vivo transfer of antigen-induced airway reactions by bronchial lumen cells.

“…Animals are young adult male and female Macaca mulatta which weigh from 2.5 to 11.5 kg. Selection and screening of animals has been described in detail (5). All BLC donors with positive respiratory responses to aerosol challenge with ascaris antigen, had a positive response defined as previously reported (5).…”

“…In a series of studies (5,7,9,11), it has been established that although the absolute base-line PF levels varied between animals (9), these levels did not change significantly (more than 10%) in an animal that had no aerosol challenge, or in any animal challenged by aerosol of PBS. Atropine did not result in more than that percentage of variation from the base line.…”

“…These included: (a) the requirement of a sufficient ntumber of bronchial lumen cells (BLC) from highly reactive donors, (b) that these cells be distributed appropriately to the airway of recipient animals, and (c) the use of an appropriate recipient animal. We have previously demonstrated that all rhesus monkeys with significant degrees of cutaneous reactivity to antigen do not have airway responses to aerosolized-antigen, and that animals with consistent airway reactivity to antigen have hyperreactive airways to analogues of acetylcholine similar to the human asthmatic (5). With these problems in mind, we developed test systems and report experiments which show that BLC from rhesus monkeys with airway reactivity to ascaris antigen will transfer this airway reactivity to recipient animals.…”

Ionophore and Arachidonic Acid Stimulation of Airway Responses in Rhesus Monkeys

“…In previous reports we have described the characteristics of the immediate-type airway response to aerosolized ascaris antigen in a group of rhesus monkeys (1,2). The limited population of animals with persistent and and pharmacologic characteristics described have led to the suggestion that the rhesus monkey antigeninduced airway response constitutes a primate model of human asthma (2,3).…”

“…The limited population of animals with persistent and and pharmacologic characteristics described have led to the suggestion that the rhesus monkey antigeninduced airway response constitutes a primate model of human asthma (2,3).…”