The search for synonyms among streptomycetes by using SDS-PAGE of whole-cell proteins. Emendation of the species Streptomyces aurantiacus, Streptomyces cacaoi subsp. cacaoi, Streptomyces caeruleus and Streptomyces violaceus

Abstract

A collection of 93 Streptomyces reference strains were investigated using SDS-PAGE of whole-cell proteins. Computer-assisted numerical analysis revealed 24 clusters encompassing strains with very similar protein profiles. Five of them grouped several type strains with visually identical patterns. DNA-DNA hybridizations revealed homology values higher than 70% among these type strains. According to the current species concept, it is proposed that Streptomyces albosporeus subsp. albosporeus LMG 19403T is considered as a subjective synonym of Streptomyces aurantiacus LMG 19358T, that Streptomyces aminophilus LMG 19319T is considered as a subjective synonym of Streptomyces cacaoi subsp. cacaoi LMG 19320T, that Streptomyces niveus LMG 19395T and Streptomyces spheroides LMG 19392T are considered as subjective synonyms of Streptomyces caeruleus LMG 19399T, and that Streptomyces violatus LMG 19397T is considered as a subjective synonym of Streptomyces violaceus LMG 19360T.

Streptomyces barkulensis sp. nov., isolated from an estuarine lake

“…They are widely known for their ability to produce a broad range of secondary metabolites, bioactive compounds and industrially important enzymes, which makes them an important genus for scientific exploration of many useful products (Bérdy, 2005; Watve et al ., 2001; Chater et al , 2010). The conventional systematics of the genus Streptomyces based on phenotypic characteristics (Williams et al , 1983; Kämpfer et al , 1991) has undergone a radical change with the application of molecular systematics (Goodfellow et al , 1992; Embley & Stackebrandt, 1994; Lanoot et al , 2002, 2004; Hatano et al , 2003; Kämpfer, 2012). Although comparison of 16S rRNA gene sequences for classification at the genus level has been widely used for Streptomyces taxonomy, differences at the species level are difficult to establish when 16S rRNA gene sequence similarities are >97 % (Stackebrandt & Goebel, 1994).…”

Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the Aminocoumarin Antibiotic Novobiocin

“…It has been in clinical use under the name albamycin and is still used in veterinary medicine. Novobiocin was discovered from a soil bacterium that was originally termed Streptomyces spheroides (2), which was later reclassified as Streptomyces caeruleus (3) and eventually as Streptomyces niveus NCIMB 11891 (4). An independent novobiocin-producing isolate was termed S. niveus NCIMB 9219 (5).…”

Description of Streptomyces fabae sp. nov., a producer of antibiotics against microbial pathogens, isolated from soybean (Glycine max) rhizosphere soil

“…Although a detailed method for determining the taxonomy of species of the genus Streptomyces based on phenotypic and physiological characteristics was provided (Williams et al , 1983; Kämpfer et al , 1991), the approach lacks credibility, especially for species that show 16S rRNA gene sequence similarities with the type species of greater than 97 % (Stackebrandt & Goebel, 1994). Thereafter, a complete method has been used employing a combination of phenotypic characteristics and molecular classification systems in biology (Goodfellow et al , 1992; Embley & Stackebrandt, 1994; Lanoot et al , 2002, 2004; Hatano et al , 2003; Rainey & Oren, 2011; Kämpfer, 2012). In this study, we focus on the description of a novel species, isolated from rhizosphere soil, with antibiotic activity that kills microbial pathogens.…”