Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the Aminocoumarin Antibiotic Novobiocin

Flinspach, Katrin; Rückert, Christian; Kalinowski, Jörn; Heide, Lutz; Apel, Alexander Kristian

doi:10.1128/genomea.01146-13

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…Streptomyces mobaraensis DSM 40847 (Yang et al, 2013) Industrial producer of transglutaminase. Streptomyces niveus NCIMB 11891 (Flinspach et al, 2014) Produces novobiocin, an aminocoumarin antibiotics. Streptomyces rapamycinicus NRRL 5491 (Baranasic et al, 2013) Produces the immunosuppressant drug rapamycin.…”

Section: Species and Strain Motivation For Sequencingmentioning

confidence: 99%

Recently published Streptomyces genome sequences

Harrison

Studholme

2014

Microbial Biotechnology

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Section: Species and Strain Motivation For Sequencingmentioning

confidence: 99%

Recently published Streptomyces genome sequences

Harrison

Studholme

2014

Microbial Biotechnology

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“…To answer this question, blast searches were performed for all 13 ROK-family proteins of S . coelicolor that contain a DNA binding domain against proteins from the native producer strain [ 27 ] ( S1 Table ). The result of this blast search demonstrates that some ROK regulators are highly conserved in both strains, as e.g.…”

Section: Resultsmentioning

confidence: 99%

Characterization of DNA Binding Sites of RokB, a ROK-Family Regulator from Streptomyces coelicolor Reveals the RokB Regulon

2016

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ROK-family proteins have been described to act either as sugar kinases or as transcriptional regulators. Few ROK-family regulators have been characterized so far and most of them are involved in carbon catabolite repression. RokB (Sco6115) has originally been identified in a DNA-affinity capturing approach as a possible regulator of the heterologously expressed novobiocin biosynthetic gene cluster in Streptomyces coelicolor M512. Interestingly, both, the rokB deletion mutants as well as its overexpressing mutants showed significantly reduced novobiocin production in the host strain S.coelicolor M512. We identified the DNA-binding site for RokB in the promoter region of the novobiocin biosynthetic genes novH-novW. It overlaps with the novH start codon which may explain the reduction of novobiocin production caused by overexpression of rokB. Bioinformatic screening coupled with surface plasmon resonance based interaction studies resulted in the discovery of five RokB binding sites within the genome of S. coelicolor. Using the genomic binding sites, a consensus motif for RokB was calculated, which differs slightly from previously determined binding motifs for ROK-family regulators. The annotations of the possible members of the so defined RokB regulon gave hints that RokB might be involved in amino acid metabolism and transport. This hypothesis was supported by feeding experiments with casamino acids and L-tyrosine, which could also explain the reduced novobiocin production in the deletion mutants.

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“…Our study reports the detection of S. mirabilis ‐like grouping, S. fulvissimus , and S. niveus , in caves. These species, which were found in stained areas of Lascaux Cave, have not been previously reported as pathogenic, but rather as producers of secondary metabolites with antimicrobial activity, some of which are used for bioremediation (El‐Sayed, 2012 ; Flinspach et al, 2014 ; Kominek, 1972 ; Myronovskyi et al, 2013 ; Saberi‐Riseh & Moradi‐Pour, 2021 ; Schütze et al, 2014 ). Regarding S. fulvissimus and S. niveus , the OTUs belonging to these species were close to the GenBank sequences obtained from torrent muddy soil (CP071044.1), rhizosphere (CP048397.1), and deep‐sea sediment (CP018047.1).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Actinobacteria communities from human‐impacted and pristine karst caves

et al. 2022

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Actinobacteria are important cave inhabitants, but knowledge of how anthropization and anthropization‐related visual marks affect this community on cave walls is lacking. We compared Actinobacteria communities among four French limestone caves (Mouflon, Reille, Rouffignac, and Lascaux) ranging from pristine to anthropized, and within Lascaux Cave between marked (wall visual marks) and unmarked areas in different rooms (Sas‐1, Passage, Apse, and Diaclase). In addition to the 16S rRNA gene marker, 441 bp fragments of the hsp 65 gene were used and an hsp 65‐related taxonomic database was constructed for the identification of Actinobacteria to the species level by Illumina‐MiSeq analysis. The hsp 65 marker revealed higher resolution for species and higher richness (99% operational taxonomic units cutoff) versus the 16S rRNA gene; however, more taxa were identified at higher taxonomic ranks. Actinobacteria communities varied between Mouflon and Reille caves (both pristine), and Rouffignac and Lascaux (both anthropized). Rouffignac displayed high diversity of Nocardia , suggesting human inputs, and Lascaux exhibited high Mycobacterium relative abundance, whereas Gaiellales were typical in pristine caves and the Diaclase (least affected area of Lascaux Cave). Within Lascaux, Pseudonocardiaceae dominated on unmarked walls and Streptomycetaceae (especially Streptomyces mirabilis ) on marked walls, indicating a possible role in mark formation. A new taxonomic database was developed. Although not all Actinobacteria species were represented, the use of the hsp 65 marker enabled species‐level variations of the Actinobacteria community to be documented based on the extent of anthropogenic pressure. This approach proved effective when comparing different limestone caves or specific conditions within one cave.

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Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the Aminocoumarin Antibiotic Novobiocin

Cited by 9 publications

References 8 publications

Recently published Streptomyces genome sequences

Recently published Streptomyces genome sequences

Characterization of DNA Binding Sites of RokB, a ROK-Family Regulator from Streptomyces coelicolor Reveals the RokB Regulon

Comparison of Actinobacteria communities from human‐impacted and pristine karst caves

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