The role of endothelin-1 and endothelin receptor antagonists in allergic rhinitis inflammation: ovalbumin-induced rat model

Tatar, Arzu; Yayla, Muhammed; Köse, Duygu; Halıcı, Zekai; Yörük, Özgür; Polat, Elif

doi:10.4193/rhin15.059

Cited by 13 publications

(5 citation statements)

References 24 publications

(38 reference statements)

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“…Antihistamines, which have an effect on all major symptoms, have been evaluated in many previous studies and their histopathological effects on the nasal mucosa have been demonstrated. In a previous study using antihistamines by Tatar et al 17 observed that edema decreased, damage to the basal membranes of the respiratory epithelium, continued cilia loss, decreased inflammatory cells, increased fibroblasts in the lamina propria, and formation of collagen fibers. Another study, 18 examined the effect of intranasal azelastine on the nasal mucosa; eosinophil and mast cell infiltration decreased, inflammatory cells decreased and there was focal goblet cell metaplasia on the respiratory epithelial surface.…”

Section: Discussionmentioning

confidence: 94%

The Role of Substance P Receptor Antagonists in Allergic Rhinitis: Ovalbumin‐Induced Rat Model

et al. 2023

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ObjectiveSubstance P is a peptide from the tachykinin family, which is found in peripheral and central nervous systems, causing vasodilation and increased secretion in the nasal mucosa. In this study, we aimed to investigate whether the experimental model of allergic rhinitis will cause allergic changes in the larynx and to compare the effects of aprepitant, a substance P antagonist, on nasal symptoms in allergic rhinitis, and histopathological changes in the nasal and laryngeal mucosa with antihistamine and leukotriene receptor antagonists (LTRA).Study DesignAn experimental animal study.MethodThe study was carried out on 34 healthy 8–12 weeks old female Sprague Dawley rats in 5 groups. The rats in which an experimental allergic rhinitis model was created with ovalbumin were scored by observing their nasal symptoms, and nasal and laryngeal mucous membranes included in the study were evaluated histopathologically after medications.ResultsAs a result of the analysis of the data obtained from the study, antihistamine and LTRA significantly reduced the symptoms of nose scratching and sneezing, while aprepitant did not affect nasal symptoms. In the histopathological examination of the larynx, effects that would make a significant difference were found in the allergy group when compared to the control group. On the larynx, aprepitant reduced pseudostratification significantly compared to the allergy group.ConclusionAprepitant provides histopathological changes in the treatment of allergic rhinitis, but does not have sufficient effect on nasal symptoms. The effect of aprepitant on the larynx has not been clearly demonstrated.Level of EvidenceNA Laryngoscope, 2023

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Section: Discussionmentioning

confidence: 94%

The Role of Substance P Receptor Antagonists in Allergic Rhinitis: Ovalbumin‐Induced Rat Model

et al. 2023

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“…Furthermore, the effectiveness of bosentan has been reported in several mouse or rat disease models. For example, in an ovalbumin‐induced allergic rhinitis rat model, oral bosentan significantly inhibited nasal symptoms and serum IgE level 37 . In addition, in a murine model of inflammatory bowel disease and a rat colitis model, bosentan significantly reduced clinical and histological inflammation 38,39 .…”

Section: Discussionmentioning

confidence: 99%

“…For example, in an ovalbumin-induced allergic rhinitis rat model, oral bosentan significantly inhibited nasal symptoms and serum IgE level. 37 In addition, in a murine model of inflammatory bowel disease and a rat colitis model, bosentan significantly reduced clinical and histological inflammation. 38,39 Moreover, in rat models of airway inflammation, bosentan also reduced the levels of eosinophils and several cytokines including IL-4, TNF-α, IFN-γ, and IL-1β in bronchoalveolar lavage fluid and lung inflammation.…”

Section: Edn1 Promotes Neuronal Elongation and Branching In Small-dmentioning

confidence: 98%

Inhibition of mite‐induced dermatitis, pruritus, and nerve sprouting in mice by the endothelin receptor antagonist bosentan

Kido‐Nakahara

Wang

Ohno

et al. 2020

Allergy

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Background: Endothelin-1 (EDN1) can evoke histamine-independent pruritus in mammals and is upregulated in the lesional epidermis of atopic dermatitis (AD). EDN1 increases the production of interleukin 25 (IL-25) from keratinocytes to accelerate T helper type 2 immune deviation. Plasma EDN1 levels are positively correlated with the clinical severity and itch intensity of AD. Therefore, we hypothesized that the inhibition of EDN1 might be useful for treating atopic inflammation and itch and investigated the effects of the topical application of the EDN1 receptor antagonist bosentan on the skin inflammation and itch in a murine AD model. Methods: We analyzed the mite-induced AD-like NC/Nga murine model, which was topically applied with bosentan or ethanol control every day for 3 weeks. We also subjected in vitro primary sensory neuron culture systems to nerve elongation and branching assays after EDN1 stimulation. Results: Topical application of bosentan significantly attenuated the development of mite-induced AD-like skin inflammation, dermatitis scores, ear thickness, scratching bouts, and serum level of thymus and activation-regulated chemokine in NC/Nga mice. Bosentan application also significantly reduced the gene expression of Il13, Il17, and Ifng in the treated lesions. Histologically, the number of infiltrated dermal cells, the epidermal EDN1 expression, and the number of intraepidermal nerve fibers were significantly inhibited upon bosentan application. While EDN1 significantly elongated the neurites of dorsal root ganglion cells in a dose-and time-dependent manner, bosentan treatment attenuated this. Conclusions: EDN1 plays a significant role in mite-induced inflammation and itch. Topical bosentan is a potential protective candidate for AD.

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“…The liver tissues (20 mg) were stabilized in an RNA stabilization reagent (RNAlater, Qiagen) and then disrupted with the TissueLyser II (Qiagen) (2x2 min). All of the RNA was purified using an RNeasy Mini Kit (Qiagen) and RNA samples were reverse-transcribed into complementary DNA with a high-capacity cDNA reverse transcription kit (Qiagen) according to our previous study [16,17] .…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Sıçanlarda CYP2E1 İnihibisyonu İle Parasetamol İntoksikasyonunda Punica granatum Çekirdek Yağı Ektraktının Hepatoprotektif Etkisi

Çetin¹,

Kiliçle²,

Güvendi³

et al. 2018

Kafkas Univ Vet Fak Derg

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How to Cite This ArticleCetin D, Aksu Kilicle P, Findik Guvendi G, Yayla M: Hepatoprotective effect of Punica granatum seed oil extract on paracetamol intoxication in rat via inhibory effect of CYP2E1. Kafkas Univ Vet Fak Derg, 24 (5): [725][726][727][728][729][730][731][732][733] 2018. AbstractPunica granatum seed oil enriched by punikalagins has been shown to have a therapeutic effect against antidiabetic, anticancer, antiinflammatory and some organ toxicities. We aimed to reveal both protective and therapeutic effects of punica granatum seed oil, which has strong antioxidant and anti-inflammatory activity on paracetamol-induced hepatic toxicity via biochemically, molecularly and pathologically in our study. Our study, 64 albino wistar rats were fasted for 24 h and then divided into 8 equal groups. Group 1: Healthy, Group 2: 2 g/kg of paracetamol (2a: 24 h, 2b: 48 h) (orally), Group 3: 140 mg/kg of n-acetylcysteine (orally) + paracetamol, Group 4: 0.32 mg/mL Punica granatum (i.p) + paracetamol, Group 5: 0.64 mg/mL Punica granatum + paracetamol, Group 6: Paracetamol + 0.32 mg/mL Punica granatum, Group 7: Paracetamol + 0.64 mg/mL Punica granatum, Group 8: Paracetamol + 140 mg/kg n-acetylcysteine. The study was terminated at 24 and 48 h after paracetamol administration. Serum ALT and AST levels were significantly increased at 24 th and 48 th h of paracetamol administration according to toxicity. While malondialdehyde, CYP2E1 and TNF-α levels also increased in the liver, superoxide dismutase and glutathione peroxidase levels decreased significantly. Increased ALT, AST levels with malondialdehyde and TNF-α levels significantly decreased by punica granatum seed oil (low doses) application and antioxidant levels were also significantly improved. Punica granatum seed oil may be used as a potential therapeutic agent in the future by strengthening the antioxidant system and preventing inflammation, especially liver toxicity due to overdose of paracetamol in suicide-battered individuals. Sıçanlarda CYP2E1 İnihibisyonu İle Parasetamol İntoksikasyonunda Punica granatum Çekirdek Yağı Ektraktının Hepatoprotektif Etkisi ÖzPunikalaginlerle zengin olan Punica granatum çekirdek yağının, antidiyabetik, antikanser, antiinflamatuar ve bazı organ toksisitelerine karşı terepötik etkileri gösterilmiştir. Çalışmamızda, parasetamol ile indüklenen hepatotoksisite üzerine güçlü antioksidan ve anti-inflamatuvar etkinliğe sahip punica granatum çekirdek yağının biyokimyasal, moleküler ve patolojik olarak koruyucu ve terapötik etkilerini ortaya koymayı amaçladık. Çalışmamızda 64 albino wistar sıçan 24 saat aç bırakıldıktan sonra 8 eşit gruba ayrıldı. Grup 1: Sağlıklı, Grup 2: Parasetamol (2a: 24 saat, 2b: 48 saat) (oral), Grup 3: 140 mg/kg n-asetilsistein (oral) + parasetamol, Grup 4: 0.32 mg/mL Punica granatum (i.p.) + parasetamol, Grup 5: 0.64 mg/mL Punica granatum + parasetamol, Grup 6: Parasetamol + 0.32 mg/mL Punica granatum, Grup 7: Parasetamol + 0.64 mg/mL Punica granatum, Grup 8: Parasetamol + 140 mg/kg n-asetilsistein. Çalışma, parase...

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The role of endothelin-1 and endothelin receptor antagonists in allergic rhinitis inflammation: ovalbumin-induced rat model

Abstract: ET-1 is one of the mediators that impact AR development and ET-1 antagonists can be useful for symptom control and for decreasing allergic inflammation in AR patients.

Cited by 13 publications

References 24 publications

The Role of Substance P Receptor Antagonists in Allergic Rhinitis: Ovalbumin‐Induced Rat Model

The Role of Substance P Receptor Antagonists in Allergic Rhinitis: Ovalbumin‐Induced Rat Model

Inhibition of mite‐induced dermatitis, pruritus, and nerve sprouting in mice by the endothelin receptor antagonist bosentan

Sıçanlarda CYP2E1 İnihibisyonu İle Parasetamol İntoksikasyonunda Punica granatum Çekirdek Yağı Ektraktının Hepatoprotektif Etkisi

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