Sıçanlarda CYP2E1 İnihibisyonu İle Parasetamol İntoksikasyonunda Punica granatum Çekirdek Yağı Ektraktının Hepatoprotektif Etkisi

Abstract

How to Cite This ArticleCetin D, Aksu Kilicle P, Findik Guvendi G, Yayla M: Hepatoprotective effect of Punica granatum seed oil extract on paracetamol intoxication in rat via inhibory effect of CYP2E1. Kafkas Univ Vet Fak Derg, 24 (5): [725][726][727][728][729][730][731][732][733] 2018. AbstractPunica granatum seed oil enriched by punikalagins has been shown to have a therapeutic effect against antidiabetic, anticancer, antiinflammatory and some organ toxicities. We aimed to reveal both protective and therapeutic effects of punica granatum seed oil, which has strong antioxidant and anti-inflammatory activity on paracetamol-induced hepatic toxicity via biochemically, molecularly and pathologically in our study. Our study, 64 albino wistar rats were fasted for 24 h and then divided into 8 equal groups. Group 1: Healthy, Group 2: 2 g/kg of paracetamol (2a: 24 h, 2b: 48 h) (orally), Group 3: 140 mg/kg of n-acetylcysteine (orally) + paracetamol, Group 4: 0.32 mg/mL Punica granatum (i.p) + paracetamol, Group 5: 0.64 mg/mL Punica granatum + paracetamol, Group 6: Paracetamol + 0.32 mg/mL Punica granatum, Group 7: Paracetamol + 0.64 mg/mL Punica granatum, Group 8: Paracetamol + 140 mg/kg n-acetylcysteine. The study was terminated at 24 and 48 h after paracetamol administration. Serum ALT and AST levels were significantly increased at 24 th and 48 th h of paracetamol administration according to toxicity. While malondialdehyde, CYP2E1 and TNF-α levels also increased in the liver, superoxide dismutase and glutathione peroxidase levels decreased significantly. Increased ALT, AST levels with malondialdehyde and TNF-α levels significantly decreased by punica granatum seed oil (low doses) application and antioxidant levels were also significantly improved. Punica granatum seed oil may be used as a potential therapeutic agent in the future by strengthening the antioxidant system and preventing inflammation, especially liver toxicity due to overdose of paracetamol in suicide-battered individuals. Sıçanlarda CYP2E1 İnihibisyonu İle Parasetamol İntoksikasyonunda Punica granatum Çekirdek Yağı Ektraktının Hepatoprotektif Etkisi ÖzPunikalaginlerle zengin olan Punica granatum çekirdek yağının, antidiyabetik, antikanser, antiinflamatuar ve bazı organ toksisitelerine karşı terepötik etkileri gösterilmiştir. Çalışmamızda, parasetamol ile indüklenen hepatotoksisite üzerine güçlü antioksidan ve anti-inflamatuvar etkinliğe sahip punica granatum çekirdek yağının biyokimyasal, moleküler ve patolojik olarak koruyucu ve terapötik etkilerini ortaya koymayı amaçladık. Çalışmamızda 64 albino wistar sıçan 24 saat aç bırakıldıktan sonra 8 eşit gruba ayrıldı. Grup 1: Sağlıklı, Grup 2: Parasetamol (2a: 24 saat, 2b: 48 saat) (oral), Grup 3: 140 mg/kg n-asetilsistein (oral) + parasetamol, Grup 4: 0.32 mg/mL Punica granatum (i.p.) + parasetamol, Grup 5: 0.64 mg/mL Punica granatum + parasetamol, Grup 6: Parasetamol + 0.32 mg/mL Punica granatum, Grup 7: Parasetamol + 0.64 mg/mL Punica granatum, Grup 8: Parasetamol + 140 mg/kg n-asetilsistein. Çalışma, parase...

Determination of Urotensin Ii Levels Before and After Lsg in Patients With Obesity

“…The homogenates were centrifuged at 5,000g for 45 minutes at 4C, and the supernatants were placed into sterile tubes and stored at -80 o C until analysis. Concentration of the total protein was determined according to the procedure described by Lowry et al [10,11].…”