The role of abscisic acid in plant tissue culture: a review of recent progress

Manoj, K.; Shekhawat, N. S.; Harish,; Gupta, Amit; Phulwaria, Mahendra; Ram, Kheta; Jaiswal, Uma

doi:10.1007/s11240-011-9923-9

Cited by 139 publications

(79 citation statements)

References 140 publications

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“…Auxin and cytokinin are considered key factors to shoot differentiation in callus culture (Skoog et al 1965;Pernisová et al 2009;Su et al 2009;Cheng et al 2010;Vanneste and Friml 2009;Zhao et al 2010). Besides, though ABA is considered an inhibitor of plant growth, while acting with other PGRs, it has a positive effect on plantlet development (Rai et al 2011;Huang et al 2012). In our previous studies, endogenous auxin, zeatin and ABA were at high levels in highly regenerable rice callus (Liu and Lee 1996;Huang et al 2012).…”

Section: Introductionmentioning

confidence: 88%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

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This study aimed to clarify the possible mechanism of endogenous phytohormone signaling and carbohydrate metabolism during shoot organogenesis induced by osmotic stress in rice (Oryza sativa L. cv. Tainung 71) callus. Non-regenerable calli derived from Tainung 71 immature embryos were inoculated on Murashige and Skoog medium containing 10 lM 2,4-D. They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium. The regeneration frequency was prominently decreased to 25 % while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), was added into the sorbitoltreated medium. It suggested that endogenous auxin signal may be involved in the induction of HRC under osmotic stress treatment. As well, HRC showed high levels of glucose, sucrose, and starch and high expression of cell wall-bound invertase 1, sucrose transporter 1 (OsSUT1), OsSUT2, PIN-formed 1, and late embryogenesis abundant 1 (OsLEA1) genes. Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment. It suggests a key role of auxin may be linked to the effect of shoot regeneration under osmotic stress treatment. Therefore, we present a putative hypothesis for regenerable calli induction by osmotic stress treatment in rice. Osmotic stress may regulate endogenous levels of auxin interacting with abscisic acid, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice.

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Section: Introductionmentioning

confidence: 88%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

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“…4 Chromatogram of HPLC on total ABA extracted from the C. campestris Fig. 5 Comparison of antibacterial activity of C. campestris extracts against Agrobacterium tumefaciens, using the agar well diffusion method (Vahdati et al 2008;Rai et al 2011). Exogenous ABA regulates synthesis of storage proteins in somatic embryos and inhibits the precocious germination of somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Amini

Deljou

Nabiabad

2016

Physiol Mol Biol Plants

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Developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. One of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. For investigating the influence of Cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to explants using Agrobacterium tumefaciens, and transgenic plants were recovered using medium supplemented with different concentration of the extract. Transgenic plants were analyzed by PCR and RT-PCR. Somatic embryos of Medicago sativa L. developed into plantlets at high frequency level (52 %) in the maturation medium supplemented with 50 mg 1 -1 C. campestris extract as compared to the medium without extract (26 %). Transformation efficiency was 29.3 and 15.2 % for medium supplemented with dodder extract and without the extract, respectively. HPLC and GC/MS analysis of the extract indicated high level of ABA and some compounds such as Phytol, which can affect the somatic embryo maturation. The antibacterial assay showed that the extract was effective against some strains of A. tumefaciens. These results have provided a scientific basis for using of C. campestris extract as a good natural source of antimicrobial agents and plant growth regulator as well, that can be used in tissue culture of transgenic plants.

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“…However, several investigations have also indicated that the addition of some adjuvants like ABA, PEG, amino acids etc. to the medium can help to overcome these problems and improve the maturation and germination of somatic embryos (Rai et al 2009(Rai et al , 2011). …”

Section: Germination Of Somatic Embryosmentioning

confidence: 99%

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Rathore

Manoj

Shekhawat

2012

Physiol Mol Biol Plants

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Factors affecting somatic embryogenesis from immature cotyledon of gum arabic tree [Acacia senegal (L.) Willd.] were investigated. Induction of somatic embryogenesis was influenced by plant growth regulator concentrations and addition of amino acids in medium. Best induction of somatic embryogenesis was obtained on MS medium supplemented with 0.45 μM 2, 4-D, 2.32 μM Kin and 15 mM L-glutamine. L-glutamine plays a significant role in the maturation of somatic embryos and most of embryos attained maturity only on L-glutamine (15 mM) containing medium. Maximum percent (75.0±2.5) germination of somatic embryos was recorded on medium containing 0.22 μM BAP.

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The role of abscisic acid in plant tissue culture: a review of recent progress

Cited by 139 publications

References 140 publications

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

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