Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Amini, Massoume; Deljou, Ali; Nabiabad, Haidar Saify

doi:10.1007/s12298-016-0374-y

Cited by 10 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Proline had no visible impact on the shoot proliferation of nodal explant of R. canina. This amino acid was previously reported to be effective in somatic embryogenesis in certain plant species [44,45]. Although proline at the maximum concentration (2 g/l) improved shoot length and leaf number, it failed to stimulate shoot regeneration in the nodal explant of R. canina and thus it is not suggested to be used for direct in vitro regeneration of this plant.…”

Section: Discussionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

show abstract

Section: Discussionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Studies of somatic embryogenesis carried out in several plant species have demonstrated this low or non-existent rate of conversion of embryos to seedlings, such as, Oplopanax elatus (Nakai) Nakai (Moon et al, 2013), Manihot esculenta Crantz (Vidal et al, 2014), Medicago sativa L. (Amini;Deljou;Nabiabad, 2016), Crataegus spp. (Taimori et al, 2016) and Coffea arabica and C. canephora (Etienne et al, 2018).…”

Section: Callus Histological Analysismentioning

confidence: 99%

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

View full text Add to dashboard Cite

Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.

show abstract

“…Растения указанного рода встречаются, например, в Ираке [2], Иране [3,4], Китае [5], Ливане [6], Нигерии [7], Египте [8,9], Индии [10,11], Италии [12,13], Тунисе [14], Турции [15,16], России [17]. Из видового разнообразия рода Cuscuta можно выделить такого представителя, как Cuscuta campestris (повилика полевая), который представляет собой интересный объект с точки зрения фитохимического изучения [18][19][20] и спектра биологической активности [21][22][23].…”

Section: Introductionunclassified

Chemical Composition And Biological Activity Of Secondary Metabolites From Cuscuta Campestris

Chiriapkin,

Zolotykh,

Kodonidi

et al. 2024

Juvenis Scientia

View full text Add to dashboard Cite

The review summarizes the literature data on the chemical composition and types of biological activity of extracts of Cuscuta campestris Yunck (field dodder), which belongs to the Convolvulaceae family. C. campestris is an aboveground parasite. Its stems wrap around the host plants and are attached to them by gaustoria, which are formed in places of contact with the feeding plant and deeply embedded in its tissue. On the territory of the Russian Federation, there are more than 30 types of dodders. Extracts of C. campestris are used in traditional medicine in South Asia, but the plant is not used as an object of biologically active compounds in Russia. It is worth noting that in recent years, studies of the pharmacological properties of C. campestris have intensified, which made it possible to establish the presence of a wide range of biological activity in the plant. For instance, the cures of field dodder exert anti-inflammatory, tranquilizing, antitumor, antipyretic, antioxidant, cerebroprotective, antifungal, antiviral, analgesic, and hepatoprotective effects. In the case of isolation of the major components of C. campestris extracts, it seemed possible to assume the importance of certain substances in the realization of their pharmacological activity, which is discussed in this review. Sugars and sugar acids, sex pheromones, tannins, carotenoids, amino acids, fatty acids and their esters, polyphenols (including flavonoids), coumarins, triterpenoids, vitamins, furans, pyrans and lactones were identified among the secondary metabolites. Due to the fact that the plant in question is widely represented on the territory of the Russian Federation and it is affordable, C. campestris is a promising object of research for the creation of new effective medicines based on it.

show abstract

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Cited by 10 publications

References 29 publications

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Chemical Composition And Biological Activity Of Secondary Metabolites From Cuscuta Campestris

Contact Info

Product

Resources

About