Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Abstract

Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming

“…The findings of this study indicate that BAP is the most effective cytokinin for shoot induction, whereas KN performed better for callus formation in Gloriosa superba L. The inclusion of cytokinin in the culture media, along with auxin, is essential for the induction of indirect adventitious shoot formation (Sen et al, 2014). Moreover, the incorporation of casein hydrolysate (CH) and coconut water (CW) in the basal media has demonstrated positive effects on callus formation (Kamaruzzaman et al, 2018), shoot morphogenesis (Samiei et al, 2021), and overall plant development (Mohammed and Ali, 2010). Furthermore, these findings are consistent with prior studies that demonstrate the advantage of BAP compared to other cytokinins, such as KN, in promoting shoot formation (Hesami et al, 2019).…”

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

“…After in-depth investigations, researchers have identified the optimal medium for in vitro regeneration, multiplication, and growth of orchid seedlings, using a combination of organic additives and growth regulators. Organic additives contain carbohydrates, vitamins, and amino acids, which enhance the effectiveness of the propagation of orchid plants and decrease laboratory production costs (Moraes et al, 2020;Poniewozik, Szot, & Parzymies, 2021;Samiei et al, 2021). However, few studies have investigated the clonal micropropagation of C. gaskelliana (Mercado & Jaimes, 2022).…”