The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods

Tynngård, Nahreen; Lindahl, Tomas; Trinks, Marie; Studer, Martina; Berlin, Gösta

doi:10.1111/j.1537-2995.2007.01610.x

Cited by 24 publications

(38 citation statements)

References 39 publications

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“…The single platelets showed no changes in GPIb expression as shown previously [13,28] but there are also studies reporting a decrease in the expression of GPIb during storage [29,30].…”

Section: Discussionmentioning

confidence: 54%

“…P-selectin increased with time as shown by us and others [6,13,31]. PS exposure increased only minimally during the normal shelf-life of PCs but by day 12, the exposure had increased significantly.…”

Section: Discussionmentioning

confidence: 75%

“…Fluorosphere control samples (Flow-Check and Flow-Set, Beckman Coulter Inc.) were used to verify that the instrument performance was stable over time. Expression of GPIb was analysed as previously described [13]. P-selectin and PS exposure was analyzed by adding 3 µL of platelet suspension, diluted as described above, to 7 mM glucose, 1 g/L bovine serum albumin and 20 mM HEPES, pH 7.40) supplemented with 2.5 mM calcium.…”

Section: Expression Of Gpib P-selectin and Phosphatidylserine (Ps) Omentioning

confidence: 99%

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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by apheresis technique (N = 7).Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage.Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analysed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation.We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.3

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“…The single platelets showed no changes in GPIb expression as shown previously [13,28] but there are also studies reporting a decrease in the expression of GPIb during storage [29,30].…”

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 75%

Section: Expression Of Gpib P-selectin and Phosphatidylserine (Ps) Omentioning

confidence: 99%

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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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“…Clotting time, maximum elasticity (G'max; final clot strength), and the time between clotting time and G'max (time to G'max) were determined as previously described . The mean change in G′ per minute showing the speed of clot G′ development (i.e., G'max/time to G'max) was calculated.…”

Section: Methodsmentioning

confidence: 99%

In vitro function of platelets treated with ultraviolet C light for pathogen inactivation: a comparative study with nonirradiated and gamma‐irradiated platelets

2014

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“…Other advantages are a wider measuring range for elasticity and the simultaneous measurement of blood viscosity [26]. Until now, the instrument has mainly been used for studies of the platelet contribution to whole blood coagulation and quality control of PCs [27][28][29]. The clinical studies published so far have been of limited size [30][31][32][33].…”

Section: Instruments Measuring Clotting and Clot Elasticitymentioning

confidence: 99%

Methods for evaluation of platelet function

Lindahl

Ramström

2009

Transfusion and Apheresis Science

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The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods

Cited by 24 publications

References 39 publications

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

In vitro function of platelets treated with ultraviolet C light for pathogen inactivation: a comparative study with nonirradiated and gamma‐irradiated platelets

Methods for evaluation of platelet function

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