Methods for evaluation of platelet function

Lindahl, Tomas; Ramström, Sofia

doi:10.1016/j.transci.2009.07.015

Cited by 20 publications

(20 citation statements)

References 37 publications

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“…Storage for 5 days did not affect the clotting time. This is in accordance with previous studies of PLTs in plasma or T‐Sol as assessed by FOR 9,11 and has also been shown for PLTs in SSP+ using thromboelastography, 27 which is a technique similar to FOR 28 . The ability of the PLTs to participate in clot retraction was slightly reduced during storage (decreased mean change in G′/min for T‐Sol and SSP+ PCs and a prolonged time to G′max for PCs with all PASs), which has previously been reported for PLTs in plasma or T‐Sol 9,11 .…”

Section: Discussionsupporting

confidence: 92%

In vitro properties of platelets stored in three different additive solutions

2011

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Section: Discussionsupporting

confidence: 92%

In vitro properties of platelets stored in three different additive solutions

2011

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“…The use of specific platelet inhibitor drugs, such as clopidogrel (platelet ADP chemoreceptor [P2Y 12 ] inhibitor), and glycoprotein (GP) IIb/IIIa inhibitors may predispose animals to bleeding. As such, there is interest in platelet function assays that are readily accessible, reliable, and cost‐effective for monitoring antiplatelet therapy or diagnosing congenital or acquired platelet disorders 99,100 …”

Section: Clinical Applications In Veterinary Medicinementioning

confidence: 99%

“…As such, there is interest in platelet function assays that are readily accessible, reliable, and costeffective for monitoring antiplatelet therapy or diagnosing congenital or acquired platelet disorders. 99,100 Platelets have multiple physiologic activators and are a major contributor to the TEG G/MA value. Thrombin is a major and powerful platelet activator, and its presence masks the detection of platelet dysfunction that results from alteration of pathways of weaker activators, such as ADP or collagen.…”

Section: Platelet Functionmentioning

confidence: 99%

Application of thrombelastography/thromboelastometry to veterinary medicine

Kol

Borjesson

2010

Veterinary Clinical Pathol

134

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Thrombelastograph analyzers are point-of-care hemostatic analyzers that provide global assessment of the hemostatic process. Thrombelastography (TEG) detects and provides a continuous recording of the changes in the viscoelastic properties of whole blood from initial clot formation through fibinolysis. TEG has been validated for use in dogs, horses, and cats. Hemostasis research using TEG has focused on test validation, alterations of TEG tracings in animals with naturally occurring diseases, and the use of TEG for monitoring various therapeutic modalities. This article reviews TEG methodology and terminology, including potential sources of preanalytical and analytical errors, the correlation between TEG and other routine hemostatic assays, and current clinical applications of TEG, with emphasis on veterinary medical practice. Data suggest that TEG may be a sensitive and useful adjunctive tool for evaluating an animal with an underlying coagulopathy, including hypercoagulability and hypocoagulability. Additional prospective studies are needed to (1) correlate TEG tracing patterns with a clinical predisposition for bleeding or thrombosis in various disease states and (2) determine whether monitoring and treating hemostatic disorders based on TEG tracings improve clinical outcome.

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“…Blood platelets are a component of the haemostatic system in which the response to an agonist can be altered by compounds formed by or stored within platelets [9]. Platelets may become activated through different pathways including ones associated with collagen and von Willebrand factor exposed to the flowing blood following vessel wall injury, adenosine diphosphate (ADP) and adenosine triphosphate released from activated platelets, or thrombin [21]. A variety of substances are important mediators of haemostasis and thrombosis, and promote platelet aggregation in vitro ; these include ADP, adrenaline, collagen, and arachinodic acid [16,26].…”

Section: Introductionmentioning

confidence: 99%

Effects of hydrocortisone and aminophylline on the aggregation of equine plateletsin vitro

et al. 2011

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The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided into three different aliquots. In the first aliquot, platelet aggregation was measured after platelet activation with 1 µM and 0.5 µM ADP (Group A). In the other two aliquots, the effect of a 10 min preincubation with hydrocortisone (Group B) or aminophylline (Group C) on ADP-induced aggregation at final ADP concentrations of 1 µM and 0.5 µM was observed. Platelet aggregation, recorded by an aggregometer, was evaluated by measuring the maximum degree of platelet aggregation and the initial velocities of platelet aggregation were obtained. Our results demonstrated the inhibitory effect of hydrocortisone and the induction effect of aminophylline on equine platelet responses in vitro.

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Methods for evaluation of platelet function

Cited by 20 publications

References 37 publications

In vitro properties of platelets stored in three different additive solutions

In vitro properties of platelets stored in three different additive solutions

Application of thrombelastography/thromboelastometry to veterinary medicine

Effects of hydrocortisone and aminophylline on the aggregation of equine plateletsin vitro

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