In vitro function of platelets treated with ultraviolet <scp>C</scp> light for pathogen inactivation: a comparative study with nonirradiated and gamma‐irradiated platelets

Tynngård, Nahreen; Trinks, Marie; Berlin, Gösta

doi:10.1111/trf.12963

Cited by 18 publications

(22 citation statements)

References 44 publications

(101 reference statements)

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“…Further, the MPV was seen to increase to a greater degree in the UVC-treated platelets during storage. This phenomenon has been reported previously with UVC-treated platelets [8,17] and may be associated with a change in the platelet from the resting discoid shape to a more spherical morphology.…”

Section: Discussionmentioning

confidence: 66%

“…There appears to be some contradictory evidence regarding the effect of UVC treatment on HSR, with some studies finding a reduction [5,8] and others not [4,6,17]. Similarly, the HSR has been shown to be negatively affected by PI treatment with other systems [10,11].…”

Section: Discussionmentioning

confidence: 99%

“…The THERAFLEX UV-Platelets system has been CE-marked, and several investigations have been conducted to determine the effect of UVC treatment on in vitro platelet quality [4,5,6,7,8]. Treatment with the UVC system appears to accelerate the metabolic rate of platelets, which is similar to the effects of other PI methods [9,10,11].…”

Section: Introductionmentioning

confidence: 87%

“…Further, the components were also at the lower end of the specified limits for the platelet concentration, and all previous studies published to date have reported higher platelet concentrations and plasma carryover than used in this study [4,5,6,8,16,17]. As both of these parameters influence the amount of UVC penetration, and thus potential platelet damage, it is imperative to establish the effect of UVC on these components.…”

Section: Discussionmentioning

confidence: 99%

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In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation

Johnson

Hyland

Tan

et al. 2015

Transfus Med Hemother

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Background: The THERAFLEX UV-Platelets system uses shortwave ultraviolet C light (UVC, 254 nm) to inactivate pathogens in platelet components. Plasma carryover influences pathogen inactivation and platelet quality following treatment. The plasma carryover in the standard platelets produced by our institution are below the intended specification (<30%). Methods: A pool and split study was carried out comparing untreated and UVC-treated platelets with <30% plasma carryover (n = 10 pairs). This data was compared to components that met specifications (>30% plasma). The platelets were tested over storage for in vitro quality. Results: Platelet metabolism was accelerated following UVC treatment, as demonstrated by increased glucose consumption and lactate production. UVC treatment caused increased externalization of phosphatidylserine on platelets and microparticles, activation of the GPIIb/IIIa receptor (PAC-1 binding), and reduced hypotonic shock response. Platelet function, as measured with thrombelastogram, was not affected by UVC treatment. Components with <30% plasma were similar to those meeting specification with the exception of enhanced glycolytic metabolism. Conclusion: This in vitro analysis demonstrates that treatment of platelets with <30% plasma carryover with the THERAFLEX UV-Platelets system affects some aspects of platelet metabolism and activation, although in vitro platelet function was not negatively impacted. This study also provides evidence that the treatment specifications of plasma carryover could be extended to below 30%.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation

Johnson

Hyland

Tan

et al. 2015

Transfus Med Hemother

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“…Beschrieben sind eine erhöhte Glykolyserate sowie ein moderater, aber signifikanter Anstieg der Annexin V-Bindung und der GP IIb/IIIa-Expression, während die Werte für p-Selektin oder Zytokinfreisetzung (TGFβ, RANTES) sich nicht von den Kontrollen unterschieden; ebenso ist die Aktivierbarkeit der Thrombozyten nicht signifikant beeinträchtigt [140,141]. Untersuchungen in der Mikroflusskammer zeigten eine verminderte Kinetik der Gerinnselbildung an Kollagen ab Lagertag 5 [142].…”

Section: Unerwünschte Effekte Der Pathogeninaktivierung Mit Theraflexunclassified

Pathogen-Inaktivierungssysteme für Thrombozytenkonzentrate

2018

Bundesgesundheitsbl

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Antimicrobial blue light for decontamination of platelets during storage

Lü

et al. 2019

Journal of Biophotonics

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Platelet (PLT) storage is currently limited to 5 days in clinics in the United States, in part, due to an increasing risk for microbial contamination over time. In light of well‐documented antimicrobial activity of blue light (405‐470 nm), we investigated potentials to decontaminate microbes during PLT storage by antimicrobial blue light (aBL). We found that PLTs produced no detectable levels of porphyrins or their derivatives, the chromophores that specifically absorb blue light, in marked contrast to microbes that generated porphyrins abundantly. The difference formed a basis with which aBL selectively inactivated contaminated microbes prior to and during the storage, without incurring any harm to PLTs. In accordance with this, when contamination with representative microbes was simulated in PLT concentrates supplemented with 65% of PLT additive solution in a standard storage bag, all “contaminated” microbes tested were completely inactivated after exposure of the bag to 405 nm aBL at 75 J/cm2 only once. While killing microbes efficiently, this dose of aBL irradiation exerted no adverse effects on the viability, activation or aggregation of PLTs ex vivo and could be used repeatedly during PLT storage. PLT survival in vivo was also unaltered by aBL irradiation after infusion of aBL‐irradiated mouse PLTs into mice. The study provides proof‐of‐concept evidence for a potential of aBL to decontaminate PLTs during storage.

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In vitro function of platelets treated with ultraviolet C light for pathogen inactivation: a comparative study with nonirradiated and gamma‐irradiated platelets

Cited by 18 publications

References 44 publications

In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation

In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation

Pathogen-Inaktivierungssysteme für Thrombozytenkonzentrate

Antimicrobial blue light for decontamination of platelets during storage

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