The production of injectable hydrazone crosslinked gellan gum-hyaluronan-hydrogels with tunable mechanical and physical properties

Karvinen, Jennika; Koivisto, Janne T.; Jönkkäri, Ilari; Kellomäki, Minna

doi:10.1016/j.jmbbm.2017.04.006

Cited by 29 publications

(25 citation statements)

References 26 publications

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“…Compression testing was performed as previously described, using a BOSE Electroforce Biodynamic 5100 machine equipped with a 225 N load sensor and Wintest 4.1 software (Bose Corporation, Eden Prairie, Minnesota, USA). Briefly, HA1‐PVA‐Col and HA2‐PVA hydrogel samples were cast into a homemade cylindrical mold with an approximate height of 6 mm and a diameter of 12 mm and stored overnight before compression testing to ensure complete gelation.…”

Section: Methodssupporting

confidence: 93%

“…As the stiffness of the 3D scaffold affects neuronal differentiation and maturation, using scaffolds with stiffness values near those of natural brain tissue is reasonable. Mechanical tests performed with the compression method showed that all the studied hydrogels had compression moduli under 10 kPa, which is in the range of brain tissue . The highest measured modulus of the hydrogels used in PCA analysis, shown in Figure 4 , was the modulus of GG (1.25% SPD) at 9.4 ± 2.7 kPa.…”

Section: Resultsmentioning

confidence: 99%

“…The data obtained from the stress–strain curve were used to estimate the so‐called stiffness of the hydrogels. Because the stress–strain curve of hydrogels (or tissues) was nonlinear in the elastic portion (even at low strains), a polynomial fit was used for the data, and the stiffness of hydrogels was determined according to the previously described method …”

Section: Methodsmentioning

confidence: 98%

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Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Ylä‐Outinen

Harju

Joki

et al. 2019

Macromolecular Bioscience

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There is a clear need for novel in vitro models, especially for neuronal applications. Development of in vitro models is a multiparameter task consisting of cell‐, biomaterial‐, and environment‐related parameters. Here, three different human origin neuronal cell sources are studied and cultured in various hydrogel 3D scaffolds. For the efficient evaluation of complex results, an indexing method for data is developed and used in principal component analysis (PCA). It is found that no single hydrogel is superior to other hydrogels, and collagen I (Col1) and hyaluronan–poly(vinyl alcohol) (HA1‐PVA) gels are combined into an interpenetrating network (IPN) hydrogel. The IPN gel combines cell supportiveness of the collagen gel and stability of the HA1‐PVA gel. Moreover, cell adhesion is studied in particular and it is found that adhesion of neurons differs from that observed for fibroblasts. In conclusion, the HA1‐PVA‐col1 hydrogel is a suitable scaffold for neuronal cells and supports adhesion formation in 3D.

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Section: Methodssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

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Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Ylä‐Outinen

Harju

Joki

et al. 2019

Macromolecular Bioscience

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“…Alternatively, HA was modified with hydrazide groups via reaction with adipic acid dihydrazide. The fabrication of the polymer product, HAADH, has been previously reported in [16]. 1 H-NMR and FTIR spectra are reported in the same article.…”

Section: Synthesis Of Hydrazide-modified Hyaluronansmentioning

confidence: 99%

“…The latter method provides a less invasive way to modify the polymer. Hydrazide groups can be produced via reaction with either adipic acid dihydrazide [15,16] or carbodihydrazide [17]. The polyanionic behavior of HA at physiological pH hinders the adhesion of proteins and cells, which can be overcome by addition of other ECM binding sites, such as collagen [18].…”

Section: Introductionmentioning

confidence: 99%

Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects

Koivusalo

Karvinen

Sorsa

et al. 2018

Materials Science and Engineering: C

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Corneal blindness is a worldwide problem, plagued by insufficient amount of high-quality donor tissue. Cell therapy using human adipose stem cells (hASCs) has risen as an alternative to regenerate damaged corneal stromal tissue, the main structural and refractive layer of the cornea. Herein we propose a method to deliver hASCs into corneal defects in hyaluronan (HA)-based hydrogels, which form rapidly in situ by hydrazone crosslinking. We fabricated two different HA-based hydrazone-crosslinked hydrogels (HALD1-HACDH and HALD2-HAADH), and characterized their swelling, degradation, mechanical, rheological and optical properties and their ability to support hASC survival. To promote hASC attachment and survival, we incorporated collagen I (col I) to the more stable HALD1-HACDH hydrogel, since the HALD2-HAADH hydrogel suffered swift degradation in culture conditions. We then used an organ culture model with excised porcine corneas to study the delivery of hASCs in these three hydrogels for stromal defect repair. Although all hydrogels showed good hASC survival directly after encapsulation, only the collagen-containing HALD1-HACDH-col I hydrogel showed cells with elongated morphology, and significantly higher cell metabolic activity than the HALD1-HACDH gel. The addition of col I also increased the stiffness and reduced the swelling ratio of the resulting hydrogel. Most importantly, the corneal organ culture model demonstrated these hydrogels as clinically feasible cell delivery vehicles to corneal defects, allowing efficient hASC integration to the corneal stroma and overgrowth of corneal epithelial cells.

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Advances in Injectable and Self‐healing Polysaccharide Hydrogel Based on the Schiff Base Reaction

Chen

2021

Macromol. Rapid Commun.

126

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Injectable hydrogel possesses great application potential in disease treatment and tissue engineering, but damage to gel often occurs due to the squeezing pressure from injection devices and the mechanical forces from limb movement, and leads to the rapid degradation of gel matrix and the leakage of the load material. The self-healing injectable hydrogels can overcome these drawbacks via automatically repairing gel structural defects and restoring gel function. The polysaccharide hydrogels constructed through the Schiff base reaction own advantages including simple fabrication, injectability, and self-healing under physiological conditions, and therefore have drawn extensive attention and investigation recently. In this short review, the preparation and self-healing properties of the polysaccharide hydrogels that is established on the Schiff base reaction are focused on and their biological applications in drug delivery and cell therapy are discussed.

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The production of injectable hydrazone crosslinked gellan gum-hyaluronan-hydrogels with tunable mechanical and physical properties

Cited by 29 publications

References 26 publications

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects

Advances in Injectable and Self‐healing Polysaccharide Hydrogel Based on the Schiff Base Reaction

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