Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Ylä‐Outinen, Laura; Harju, Venla; Joki, Tiina; Koivisto, Janne T.; Karvinen, Jennika; Kellomäki, Minna; Narkilahti, Susanna

doi:10.1002/mabi.201900096

Cited by 18 publications

(21 citation statements)

References 53 publications

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“…Principal component analysis (PCA) was used as previously described 87 to segregate MEA results from different experiments. The data were derived from two differentiations of hESC line 08/023 (named hPSC 1 and 2) and one differentiation of hiPSC line 10212.EURCCs (named hPSC 3).…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Hyvärinen

Hyysalo

Kapucu

et al. 2019

Sci Rep

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Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in vitro modeling of neurological diseases and for advancing drug development and neurotoxicological studies. However, generating electrophysiologically mature neuronal networks from hPSCs has been challenging. Here, we report the differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate. We apply microelectrode array (MEA) measurements to assess network events and compare the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures. In both of these networks, activity developed through a similar sequence of stages and time frames; however, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions.

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Section: Methodsmentioning

confidence: 99%

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Hyvärinen

Hyysalo

Kapucu

et al. 2019

Sci Rep

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“…Recombinant LN-521 has been widely used for stem cell expansion and the generation of neural progenitor cells for disease models and stem cell therapies. [15,48,49] We cross-linked the LN-521 presenting hydrogels by bioorthogonal copper-free click chemistry, which enabled tuning of material properties and creation of biologically relevant microenvironments evidenced by supported encapsulation and culture of both human neuroblastoma cells (SH-SY5Y) and human induced pluripotent stem cell (hiPSC)-derived long-term neuroepithelial stem cells (lt-NES). Furthermore, the hydrogels' favorable and tunable rheological properties provided a protective effect on the lt-NES during syringe extrusion in an in vitro model for cell injection therapy.…”

Section: Introductionmentioning

confidence: 99%

Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication

Jury

Matthiesen

Boroojeni

et al. 2021

Preprint

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Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches. LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons towards development of advanced tissue models and cell-based therapies for the treatment of neurological disorders. Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation. Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials. Here we show a modular hydrogel system for neural 3D cell culture, based on hyaluronan (HA) and poly(ethylene glycol) (PEG), that is cross-linked and functionalized with human recombinant LN 521 using bioorthogonal copper-free click chemistry. Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids. Neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN. The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of syringe extruded lt-NES, which can facilitate the development of advanced neuronal tissue and disease models and translation of stem cell-based therapies.

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“…The adhesion of neurons varies from that of the fibroblasts. The results showed that the synthesized hydrogel acts as a proper scaffold for neuronal cells [115]. Many humans suffer from blindness as a result of corneal diseases.…”

Section: Medical Applicationsmentioning

confidence: 92%

Interpenetrating polymeric hydrogels as favorable materials for hygienic applications

2020

Biointerface Res Appl Chem

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Polymers can crosslink to produce intermingled materials with three-dimensional network structure known as interpenetrating polymeric network (IPN). They comprise elastic crosslinked polymeric chains. The chains of the hydrogels are either physically or chemically entangled together. Interpenetrating hydrogels can be tailored to provide enhanced materials. They can be classified according to methods of their synthesis as simultaneous or sequential IPNs and the structure to be homo or semi IPNs. The preparation factors play a role in controlling the properties of the produced IPNs. Moreover, the ambient conditions such as pH, temperature as well as the ionic strength may affect the performance of these hydrogels. The swelling capacity is an important feature that allows the prepared hydrogel to perform the required application. Some disadvantages may arise such as the low mechanical properties that are suggested to be overcome. IPNs can be used in various applications that serve the human requirements like drug delivery, tissue engineering, medical and packaging applications. Hydrogels present biocompatibility and nontoxicity when used in biomedical applications. Interpenetrating hydrogels can be prepared from natural or synthetic polymers. Polysaccharides as natural polymers can be used to produce efficient interpenetrating hydrogels. Polyacrylates, poly(ethylene glycol) and poly(vinyl alcohol) are designated as promising synthetic polymers capable of forming interpenetrating hydrogels.

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Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Cited by 18 publications

References 53 publications

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication

Interpenetrating polymeric hydrogels as favorable materials for hygienic applications

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