2018
DOI: 10.1016/j.msec.2017.12.013
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Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects

Abstract: Corneal blindness is a worldwide problem, plagued by insufficient amount of high-quality donor tissue. Cell therapy using human adipose stem cells (hASCs) has risen as an alternative to regenerate damaged corneal stromal tissue, the main structural and refractive layer of the cornea. Herein we propose a method to deliver hASCs into corneal defects in hyaluronan (HA)-based hydrogels, which form rapidly in situ by hydrazone crosslinking. We fabricated two different HA-based hydrazone-crosslinked hydrogels (HALD1… Show more

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Cited by 52 publications
(42 citation statements)
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References 45 publications
(55 reference statements)
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“…Koivusalo et al demonstrated using a porcine organ culture model that modified hyaluronic acid based hydrogels seeded with adipose derived stem cells could be used to repair damaged cornea without the need for sutures. [151] Hyaluronic acid has also been suggested as a suitable xeno-free substrate for culturing corneal epithelial cells [149] and as a potential carrier for endothelial cells. [163,164] Despite some promising results, one potential limiting factor with using hyaluronic acid for corneal tissue regeneration is that the material has been associated with lymphangiogenesis in the limbus, although this requires further study.…”
Section: Hyaluronic Acidmentioning
confidence: 99%
“…Koivusalo et al demonstrated using a porcine organ culture model that modified hyaluronic acid based hydrogels seeded with adipose derived stem cells could be used to repair damaged cornea without the need for sutures. [151] Hyaluronic acid has also been suggested as a suitable xeno-free substrate for culturing corneal epithelial cells [149] and as a potential carrier for endothelial cells. [163,164] Despite some promising results, one potential limiting factor with using hyaluronic acid for corneal tissue regeneration is that the material has been associated with lymphangiogenesis in the limbus, although this requires further study.…”
Section: Hyaluronic Acidmentioning
confidence: 99%
“…Aldehyde groups were introduced to HA either by using periodate oxidation (HA1) or selective oxidation of diol‐modified HA (HA2). The syntheses and determinations of the degree of substitution (DS%) of modified HA1 and HA2 components were carried out according to previously reported procedures . Hydrazide groups were introduced to PVA using glycine ethyl ester and hydrazine as a source of the hydrazide unit.…”
Section: Methodsmentioning
confidence: 99%
“…Delivered stem cells were integrated into the cornea within 7 days, and the repopulation of epithelial cells was reported within this period. Longer preclinical observations are required to investigate the efficiency of hASCs delivery for treating corneal stromal defects [134].…”
Section: 222mentioning
confidence: 99%