The principles and applications of nano-diagnosis system for a nano-biosensor

Abstract

In this review, the basic principles and research trends of biosensors are briefly described and a nano-sensing system applying QCM (quartz crystal micro-balance), nano-diagnosis methods by AFM (atomic force microscopy) and SNOAM (scanning near-field/atomic force microscopy) is discussed intensively. The principle, construction, and applications of piezoelectric crystal sensors as a universal sensor are reviewed. This review is focused mainly on liquid phase applications, such as immune-sensors, gelation detecting sensors, and cultured cell monitoring sensors. The principle of nano-diagnosis based on the AFM or SNOAM techniques is described in detail. Finally, the binding affinity of peptide probes to proteins using AFM and the visualizing of a hybridized PNA probe on a DNA molecule using SNOAM are evaluated and discussed.

Piezoelectric Biosensors for Organophosphate and Carbamate Pesticides: A Review

“…However, the application range of the quartz crystal is in progress. New measuring techniques such as atomic force microscope (AFM) that use the quartz crystal as a transducer for chemical sensors and biosensors has been also developed [ 40 ]. New research results are expected to be continuously announced.…”

Investigating Chiral Recognizability of Diastereomeric Crystallization of Mandelic Acid and L-Phenylalanine

“…45 Normally, the phase lag reveals the different adhesion force, friction, and viscoelasticity. Thus, a strong adhesive force can produce a strong phase lag signal, meaning a strong binding interaction.…”

Evaluation of Interaction Between Histidine Binding Cu2+ Ion and Histidine by Atomic Force Microscopy

“…The principles of measuring affinity between biomolecules using AFM force-distance curve are documented in several reports. [1][2][3][4][5][6][7][8] The horizontal axis of force-distance curve represents the distance between the specimens and the tip, and the vertical axis represents the bending degree of the cantilever, namely, force applied to the cantilever. When the substrate and the cantilever are separated each other, the force is not applied to the cantilever.…”

“…In this way, AFM can measure unfolding DNA-DNA interaction and ligand-receptor interaction with highly accurate pN level. 1 To investigate the possibility of assessing interactions between biomolecules, AFM force-distance curve between the histidine-modified cantilever and the histidine-modified substrate in the conditions with and without Cu 2+ intercalating ion was measured and interpreted via Gaussian curve fitting analysis.…”