The primary isolation of<i>Leishmania donovani</i>from Iraq on different culture media

Rassam, Maysoon; Al-Mudhaffar, Sami A.

doi:10.1080/00034983.1979.11687268

Cited by 5 publications

(2 citation statements)

References 3 publications

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“…The culture was microscopically examined for the presence of the promastigote stage after 7-10 days. It was considered negative if absence of this stage during the first 4 weeks (Rassam and Al-Mudhaffar 1979).…”

Section: Isolation and Regrowth Percentage Of Leishmania Tropica Paramentioning

confidence: 99%

Comparative immune study on cutaneous leishmaniasis patients with single and multiple sores

2013

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Ninety-five Iraqi patients with cutaneous leishmaniasis (CL) caused by Leishmania tropica at AL-Karama Hospital in Baghdad were included in this study. Sixty patients were with single sore and the remaining with multiple sores. The study also included 10 atopic patients and 30 healthy individuals as a control group. Cellular and humoral immune response at different stages of the disease activity (early and late) were evaluated by estimation of serum IFNc, IL-4 and total IgE antibodies using ELISA kits while, the detection of specific anti leishmanial IgE antibodies was done manually. Specific IgE antibodies were only detected in early CL (\2 months) patients 68 (71.57 %) while, were not detected in late CL, atopic and healthy controls 30 (100 %). The results also showed a positive relationship between this antibody and the number of sores. Th-2 predominates during the early stage of the disease then shifts to Th-1 that proceed in the late stage, but both cytokines increased in CL patients in comparison to control group. The immune response of CL infection is possibly regulated by both Th-1 and Th-2. Multiple sores patients showed an increase of anti leishmanial IgE (0.120 ± 0.014), total IgE (120.7 ± 39.58 IU/ml), IFN-c (87.4 ± 30.52 pg/ml) and IL-4 (63.70 ± 20.32 pg/ml) levels than single sore patients with mean value of 0.108 ± 0.14, 92.3 ± 35.23 IU/ml, 47.2 ± 27.80 pg/ml and 51.04 ± 15.0 pg/ml respectively. It can be presented also as ratio of INF-c/IL-4 = 1.37 which is greater than those for single sore 0.9. These results indicated that the immune response of multiple sores patient's is higher than that with single sores.

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Section: Isolation and Regrowth Percentage Of Leishmania Tropica Paramentioning

confidence: 99%

Comparative immune study on cutaneous leishmaniasis patients with single and multiple sores

2013

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“…All these precautions were taken so that possible sources of contamination were cut down to the lowest level. (Rassam and AL-Mudhaffar 1979).…”

Section: Parasitological Diagnosismentioning

confidence: 99%

Lactate dehydrogenase (LD), alkaline phosphatase (ALP) isoenzymatic patterns in Iraqi children with visceral leishmaniasis before and after treatment with stibogluconate

et al. 2014

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The mean levels of alkaline phosphatase (ALP), lactate dehydrogenase enzymes exhibited a significant elevation in visceral leishmaniasis (VL) patients compared to the control. There was no significant change in relation to the sex and age. ALP isoenzymes revealed three banding patterns which differ from the three zymodems which were obtained from control group. These differences may be due to isoenzymes activity of patients with VL before and after therapy. Lactate dehydrogenase (LD) isoenzymes revealed five banding patterns differ from the five normal zymodems. These differences mainly occurred due to LD isoenzymes activity in patients with VL before and after therapy.

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