An in vitro method for long-term maintenance of Leishmania donovani chagasi

Beltrán, Sofía Duque; Tesh, Robert B.; Carvajal, Dioselina Peláez; Arjona, Augusto Corredor

doi:10.1016/0035-9203(88)90427-0

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…Several reports have indicated the requirement of inorganic salts, glucose and amino acids for the propagation of the leishmanial promastigotes (Steiger and Steiger, 1997, Chaudhuri et al, 1986, Beltran et al, 1988. In addition, purine as well as many vitamins and related growth factors including folic acid and hemin are essential for Leishmania (Steiger and Meshnick, 1977, Merino and Gabaldon, 1978, Melo et al, 1985, Lemesre et al, 1988.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Cultivation of Promastigotes of Iraqi Leishmanial Species in Serum-Free Liquid Medium Containing Human Urine

Hassan¹,

Shakoor²,

Mahmood³

2016

Int.J.Curr.Res.Aca.Rev.

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Section: Resultsmentioning

confidence: 99%

In Vitro Cultivation of Promastigotes of Iraqi Leishmanial Species in Serum-Free Liquid Medium Containing Human Urine

Hassan¹,

Shakoor²,

Mahmood³

2016

Int.J.Curr.Res.Aca.Rev.

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Folic acid the essential supplement to brain heart infusion broth for cultivation and cloning of Leishmania donovani promastigotes

Kar

1997

Parasitology

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Multiplication of virulent and avirulent Leishmania donovani promastigotes was inhibited by brain heart infusion broth (BHI) (3.7%) supplemented with haemolysed or whole blood (2.5-10%) or heat-inactivated foetal bovine serum (HIFBS) (10-20%). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for the reversal of this growth inhibition. Supplementation of BHI with folic acid further supplemented with haemolysed rabbit blood or HIFBS and haemin supported propagation in serial subpassages of 2 primary isolates and 3 other virulent strains of L. donovani and 1 strain of L. major. Folic acid supplemented BHI with 3% rabbit blood in solid phase with 0.7% agar also supported the colony formation of L. donovani. This manuscript documents the development of high efficiency media for the cultivation of L. donovani promastigotes in liquid phase and as colonies on agar plates using BHI as a basal medium.

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Experimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera:Psychodidae)

Bello

Mejía

Corena

et al. 2005

Mem. Inst. Oswaldo Cruz

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The present work describes the in vitro infection of a cell line Lulo, derived fromLeishmania chagasi is a causative protozoan parasite of American visceral leishmaniasis (Grimaldi & Tesh 1993). The disease is endemic in some regions of Colombia (Corredor et al. 1989(Corredor et al. , 1990 and throughout all the South and Central America (Grimaldi & Tesh 1993, Tanner 1996; the parasite has been isolated from hosts, reservoirs and sand fly vectors. This species, as well as other species belonging to the Leishmania genus, develops through a digenetic life cycle consisting of an extracellular promastigote form that develops and multiplies in the alimentary tract of the sand fly vector and an intracellular amastigote form that resides and multiplies inside the infected mammal host's macrophages. Clinical and experimental evidence indicates that vector, parasite, and host factors influence the evolution and outcome of the infection caused by Leishmania parasites (Grimaldi &Tesh 1993).Promastigotes are routinely cultured in vitro using cell free medium; such easily obtained media have also been used to induce promastigote transformation to amastigotelike forms in response to elevated temperature and acid pH (Hendrick et al. 1978, Hunter et al. 1982, Pan 1984, Zilberstein et al. 1991, Bates et al. 1992, Bates & Tetley 1993, Zilberstein & Sahapira 1994, Bates 1994 , Hodgkinson 1996, Saar et al. 1998. It has been shown that these axenic amastigotes closely resemble animal-derived amastigotes. Leishmania in vitro differentiation, maturation, and replication has also been achieved in both macrophage cell cultures (Chang & Dwyer 1978, Chang 1979, Pearson et al. 1981, Aikawa et al. 1982 and non-macrophage cell lines, mainly in fibroblast cultures (Mattock & Petters 1975, Chang 1978, Dedet et al. 1983, Schwartzman & Pearson 1985, Corte-Real et al. 1995, Hervás Rodrigues et al. 1996, Pessotti et al. 2004. However, in spite of current support, cell lines and cell free media being available for transforming the life cycle of diferent Leishmania species, there is no information available regarding this process in cell cultures obtained from phlebotomine sand fly vectors.The present study describes the in vitro infection of Lulo cell line (Rey et al. 2000) by L. chagasi promastigotes and some ultrastructural characteristics regarding the parasite's interaction with these cells were determined. This cell line was derived from L. longipalpis embryonic tissue, which is the main vector of L. chagasi in its natural environment. The experimental infection of Lulo cell line was compared to infection of J774 cell (Ralph et al. 1975). MATERIALS AND METHODSParasites -The strain of L. chagasi, MH/CO/84/ Cl-044B, was isolated from a human case of visceral leishmaniasis in Quipile, Cundinamarca, Colombia. This strain has been maintained by periodic inoculations of promastigotes in Syrian golden hamsters (Mesocricetus auratus) in the parasitology laboratory at the National Institute of Health in Colombia. L. chagasi amastigotes were recovered...

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An in vitro method for long-term maintenance of Leishmania donovani chagasi

Cited by 3 publications

References 3 publications

In Vitro Cultivation of Promastigotes of Iraqi Leishmanial Species in Serum-Free Liquid Medium Containing Human Urine

In Vitro Cultivation of Promastigotes of Iraqi Leishmanial Species in Serum-Free Liquid Medium Containing Human Urine

Folic acid the essential supplement to brain heart infusion broth for cultivation and cloning of Leishmania donovani promastigotes

Experimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera:Psychodidae)

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