A new serum free liquid culture medium containing sterile fresh human urine was described for in vitro cultivation of Iraqi leishmanial promastigotes. The medium is simple to prepare, easy to purchase and successful to obtain bulk cultivation and maintenance of Leishmania donovani, L. tropica and L. major promastigotes.
In this study a total of 600 suspected cases of cutaneous leishmaniasis at refugee campus in Kirkuk were investigated. Samples were collected during the period of January-March 2015 ,pateints ages were (1-50) years old. Fifty four cases were found to be positive with infection rate 9%. Cutaneous leishmaniasis were more frequent in females (61.11%) than in males (38.88%). The highest infection rate of cutaneous disease (37.07%) were found among age group (1-5) years where the lowest infection rate (1.85%) were found at age groups (31-35 and 46-50) years. Also the results showed that the infected cases with single sore(66.66%) were more frequent than with multiple sores (33.32%). Statistical analysis showed that the mean value of hemoglobin was significant decrease in the infected cases when compared to the control groups (P<0.05). Furthermore , the differential count of leukocytes, neutrophil, monocytes and lymphocytes were significant increased significantly in patients with cutaneous leishmaniasis as compared to control groups. So may can consider the parameters above as a biomarker to identify the disease.
Serum levels of IL-17A, IL-33, zinc, iron and adenosine deaminase were assessed in patients with cutaneous leishmaniasis. IL-17A, IL-33 and adenosine deaminase were significantly increased in patients compared to healthy subjects. In contrast zinc and iron were decreased in sera of patients. These results suggest a role of IL-17A and IL-33 in the pathogenesis of cutaneous leishmaniasis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.