In Vitro Cultivation of Promastigotes of Iraqi Leishmanial Species in Serum-Free Liquid Medium Containing Human Urine

Abstract

A new serum free liquid culture medium containing sterile fresh human urine was described for in vitro cultivation of Iraqi leishmanial promastigotes. The medium is simple to prepare, easy to purchase and successful to obtain bulk cultivation and maintenance of Leishmania donovani, L. tropica and L. major promastigotes.