The presence of antibiotic resistance genes and bft genes as well as antibiotic susceptibility testing of Bacteroides fragilis strains isolated from inpatients of the Infant Jesus Teaching Hospital, Warsaw during 2007–2012

Kierzkowska, Marta; Majewska, Anna; Szymanek-Majchrzak, Ksenia; Sawicka-Grzelak, A.; Młynarczyk, A; Młynarczyk, G.

doi:10.1016/j.anaerobe.2019.03.003

Cited by 19 publications

(26 citation statements)

References 48 publications

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“…It is pertinent to note that no bft-4 isotype strain was detected in this series. This order of prevalence of the isotypes is partially concordant with previous reports by Scotto d'Abusco et al, [27], Sarvari et al, [11] and Kierzkowska et al, [25]. In their study, Sarvari and colleagues [11] from Hungary reported the prevalence of 10% for the bft-1 isotype compared with 3% bft-2 but, unlike our study, they did not detect bft-3 isotype.…”

Section: Discussionsupporting

confidence: 93%

“…It has been shown that bft-positive B. fragilis are more invasive than bft-negative isolates in different types of infections and that blood culture isolates are more likely to carry bft enterotoxin gene than other isolates [24]. The prevalence of 49.9% for the enterotoxin producing-extra intestinal B. fragilis in our study is relatively high when compared with the figures of 14.4% reported in Poland [25], 18.6%, in Japan [16], 6.2-38% in USA [6,7], and 13-25% in Hungary [11,26]. Our data showed that the majority (81.4%) of the isolates contained bft-1 isotype compared with 15.7% of bft-2 and 2.9% bft-3 isotypes.…”

Section: Discussioncontrasting

confidence: 64%

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Prevalence and antimicrobial susceptibility of enterotoxigenic extra-intestinal Bacteroides fragilis among 13-year collection of isolates in Kuwait

et al. 2020

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Background: Some strains of Bacteroides fragilis species are associated with diarrhea as a result of enterotoxin production (bft or fragilysin). Fragilysin is activated by C11 protease (fpn) and together with C10 protease (bfp) play a significant role in its invasiveness. The objectives of this study were to investigate the proportion of clinical isolates from extra-intestinal sources that are toxin producers and characterize the genes mediating toxin production. Clinical isolates submitted to our reference laboratory over the last 13 years were screened for toxin production using PCR technique. All stool isolates were excluded. The isolates were tested for their susceptibility to 8 antimicrobial agents by E test. Carbapenem resistance gene cfiA was detected by PCR. Results: A total of 421 B. fragilis isolates were viable. Out of these, bft was detected in 210 (49.9%) isolates. Of the 210 bft-positive isolates, 171 (81.4%), 33 (15.7%) and 6 (2.8%) harbored bft-1, bft-2, and bft-3 genes, respectively. Twenty (9.5%) of the bft-positive strains originated from bloodstream infections. Twenty-five, 20 and 9 strains harbored bfp-1, bfp-2 and bfp-3 gene, respectively. Two, 3, 4 bfp isotypes were detected simultaneously in some of strains. The resistance rates against amoxicillin-clavulanic acid was 32%, clindamycin 62%, cefoxitin 26%, imipenem 11%, meropenem 17%, metronidazole 4%, piperacillin 61% and tigecycline 14%. A chromosomally located cfiA gene that encode metallo-β-lactamase was identified in only 34 isolates (16.2%). Conclusions: The prevalence of enterotoxin-producing B. fragilis was high among the extra-intestinal isolates. Metronidazole was the most active agent against all isolates. There was no statistically significance difference between resistance rates among bft-positive and bft-negative isolates except for clindamycin.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 64%

Prevalence and antimicrobial susceptibility of enterotoxigenic extra-intestinal Bacteroides fragilis among 13-year collection of isolates in Kuwait

et al. 2020

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“…The resistance rate of B. fragilis to metronidazole in this study was 7.5%, which is obviously higher than previous reports [5,6,7,9,21,22], and no metronidazole resistant non-B. fragilis isolate was detected.…”

Section: Antibiotic Susceptibility Test Resultscontrasting

confidence: 83%

“…fragilis isolates harboured cepA, which was obviously different with those reported in some previous reports [5,19,20]. Kierzkowska M et al [9] reported that in B. fragilis, cefoxitin phenotypic resistance was strongly correlated with cfxA gene expression. However, only 2 B. fragilis isolates and 2 non-B.…”

Section: Antibiotic Susceptibility Test Resultscontrasting

confidence: 79%

“…isolates to these kinds of antibiotics was related with some specific resistance genes. nim gene was found to be closely correlated with metronidazole [8], while the presence of cfxA gene was strongly related with cefoxitin resistance [9]. Macrolide-Lincosamide-Streptogramin B resistant determinants, erm genes are widely distributed among Bacteroides spp.…”

Section: Introductionmentioning

confidence: 94%

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Higher prevalence of multi-antimicrobial resistant Bacteroides spp. strains isolated at a tertiary teaching hospital, China

Wang¹,

Han²,

Shen³

et al. 2019

Preprint

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Background: The antimicrobial resistance of Bacteroides spp. isolates is reported to be increasing among different districts around the world, and few reports found that multi-antimicrobial resistant strains emerged. However, less is known about the prevalence of multi-antimicrobial resistant isolates in China, especially for carbapenem resistance. Methods: Bacteroides spp. clinical strains were isolated from inpatients at a 3000 bed tertiary teaching hospital , and were identified by MALDI-TOF MS and VITEK-2 anaerobes and corynebacterium (ANC) card. Broth microdilution method was employed to detect the antimicrobial sensitivities of Bacteroides spp. isolates and PCR method was used to detect the resistance genes, including cfxA, cepA, cfiA, ermF and nim. The upstream insertion sequence (IS) element of cfiA gene was further detected and verified. Results: Among 115 Bacteroides spp. strains enrolled in this study, 80 isolates were Bacteroides fragilis and 35 isolates were non-Bacteroides fragilis. The total resistance rates of 115 Bacteroides spp. isolates to ampicillin/sulbactam, amoxicillin/clavulanic, cefoxitin, piperacillin, piperacillin/tazobactam, moxifloxacin, clindamycin, metronidazole, imipenem and meropenem were 22.6%, 19.6%, 3.5%, 27.8%, 8.7%, 16.5%, 80.0%, 5.2%, 13.9% and 13.9% , respectively. Except cefoxitin and moxifloxacin, the resistance rates of B. fragilis isolates to the above antibiotics were all higher than those of non-B. fragilis isolates. The positive rates of carbapenem resistance gene cfiA were 38.9% and 8.6% for B. fragilis and non-B. fragilis isolates, respectively. For 15 carbapenem resistant B. fragilis isolates, the co-carrying rates of carbapenem resistance gene cfiA and its upstream IS element were 86.7% (13/15). Conclusions: The overall resistance rates of Bacteroides spp. isolates toward multiple antibiotics were at a higher level, especially for B. fragilis. CfiA gene carrying rate among B. fragilis isolates was high up to 38.9% and its mediated carbapenem resistance was a major resistance mechanism for B. fragilis. The findings of this study imply that the actual resistance tendency of Bacteroides spp. may be underestimated and need to be given more attention to.

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What we actually know about the pathogenicity of Bacteroides pyogenes

Majewska

Kierzkowska

Kawecki

2021

Med Microbiol Immunol

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The aim of the study was to evaluate the pathogenic potential of Bacteroides pyogenes, rarely identified in clinical laboratories anaerobic bacteria. To increase the knowledge about this poorly understood anaerobic microorganism, the study also includes cases of infections described so far in the literature. Only the use of 16S rRNA sequencing and mass spectrometry technique allowed the identification of B. pyogenes from clinical specimens. We reported 13 severe human infections caused by B. pyogenes. Bacteria were cultured from the wound after biting by animals, chronic infections within the oral cavity, from patients with histologically or radiological proven osteomyelitis, surgical site infection, and from urine sample collected after a urological procedure. Most (9/13) of the patients required hospitalization. Almost 70% of them needed urgent admission via the emergency room. Two inpatients due to a life-threatening condition were admitted to the intensive care unit. Almost 50% of isolates were resistant to penicillin. All resistant to penicillin strains were isolated from skin and mucous membrane infections.

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The presence of antibiotic resistance genes and bft genes as well as antibiotic susceptibility testing of Bacteroides fragilis strains isolated from inpatients of the Infant Jesus Teaching Hospital, Warsaw during 2007–2012

Cited by 19 publications

References 48 publications

Prevalence and antimicrobial susceptibility of enterotoxigenic extra-intestinal Bacteroides fragilis among 13-year collection of isolates in Kuwait

Prevalence and antimicrobial susceptibility of enterotoxigenic extra-intestinal Bacteroides fragilis among 13-year collection of isolates in Kuwait

Higher prevalence of multi-antimicrobial resistant Bacteroides spp. strains isolated at a tertiary teaching hospital, China

What we actually know about the pathogenicity of Bacteroides pyogenes

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