The Preparation of Sodium Ribonucleate with the Use of Sodium Dodecyl Sulfate<sup>1</sup>

Kay, Ernest; Dounce, Alexander L.

doi:10.1021/ja01112a047

Cited by 78 publications

(23 citation statements)

References 4 publications

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“…Isolation of nucleic acids and deoxyribonucleohistone (DNP).-DNA was isolated by the methods of Marko and Butler (1951) and Kirby (1957), and RNA by the methods of Kay and Dounce (1953) and Kirby (1956). Any modifications that were introduced into these procedures are mentioned in the text.…”

Section: Analysis Of Metal-induced Tumoursmentioning

confidence: 99%

“…The microsomes were recovered by centrifugation for 1 hr. at 105,000 g (Spinco Model L Centrifuge), the residual supernatant solution being termed the soluble fraction.Additional preparations of cell nuclei only were made by the lissapol method of Gilbert and Radley (1964).Isolation of nucleic acids and deoxyribonucleohistone (DNP).-DNA was isolated by the methods of Marko and Butler (1951) and Kirby (1957), and RNA by the methods of Kay and Dounce (1953) and Kirby (1956). Any modifications that were introduced into these procedures are mentioned in the text.…”

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confidence: 99%

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Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Heath¹,

Webb²

1967

Br J Cancer

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PREVIOUS studies in this laboratory have shown that cobalt, nickel and cadmium, when injected as the powdered metal into the thigh muscle of rats of the hooded strain, produce a high incidence of rhabdomyosarcomata, whereas a number of other metals, including iron, copper, zinc, manganese, beryllium and tungsten are not carcinogenic under these conditions (Heath, 1956(Heath, , 1960 Heath and Daniel, 1964a, b;Heath, Daniel, Dingle and Webb, 1962). Tumours, which arise at any time from about 3 months after implantation of the active metals, and which metastasize readily in the primary animal, are transplantable into rats of the same strain. A cobalt-induced tumour, for example, has been maintained by transplantation for 13 years and is now in its 137th passage. Corresponding values for a nickel-induced and cadmium-induced tumour are 31 years and 54th transplant, and 6 years and 75th transplant respectively. All of these transplants preserve the characteristics of the primary rhabdomyosarcomata; in general the cobalt primary tumours are the least and the nickel tumours the most differentiated.Since a metallic implant disappears from the site of injection, it is probable that the metal in the dissolved state is one of the main factors in the induction of malignancy. Ions of many of the above mentioned metals, whether carcinogenic or not, are inhibitory to tissue metabolism (e.g. Dingle, Heath, Webb and Daniel, 1962) and are toxic to chick and mammalian cells in culture (Heath, 1953;Daniel, 1961Daniel, , 1964; no common specific biochemical activity of Co2+, Ni2+ and Cd2+ has yet been demonstrated.The work that is reported in this paper is designed to answer the following questions. Firstly, whether the tumours contain high local concentrations of the corresponding metallic ions; secondly, whether these ions are bound firmly and are retained after the dissolution of the metal implants, and, thirdly, whether the carcinogenic cations have a common pattern of distribution, and are associated primarily with a particular intracellular component or organelle. MATERIAL AND METHODSBiological methods.-Primary tumours were induced by the injection of a suspension of the finely powdered metal (28 mg.; Johnson, Matthey & Co. Ltd., Hatton Garden, London, E.C.1.) in horse serum (0.4 ml.) into the thigh muscle of 2-3 month old rats of the hooded strain as described by Heath (1956) and Heath and Daniel (1964a, b). Tumours for biochemical work were taken usually between 4 and 5 months after the initial implantation. At this stage of development they were free from any significant central necrosis. ANALYSIS OF METAL-INDUCED TUMOURSTransplants were maintained by serial passage of tissue fragments (about 1.5 mm.3) from the edge of an actively growing tumour into fresh rats at intervals of about 3-4 weeks. Insertion of the tumour tissue into the thigh muscle was made with a large-bore hypodermic needle.All experimental animals were fed ad libitum on Diet No. 86 (North-Eastern Agricultural Co-operative Society Ltd.) rat cubes, and had...

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Section: Analysis Of Metal-induced Tumoursmentioning

confidence: 99%

mentioning

confidence: 99%

Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Heath¹,

Webb²

1967

Br J Cancer

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“…I n this calculation of &, , no account is taken of possible effects of heating RNA and subsequently measuring the optical density in 10 yo NaCl. To investigate this, a sample of RNA was prepared from guinea-pig liver by the method of Kay & Dounce (1953). The purified preparation contained 8.4 yo phosphorus and had an &, (in water) of 9300.…”

Section: Estimation Of Virus Nucleic Acid ( Vna)mentioning

confidence: 99%

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

Ada

Perry

1956

Journal of General Microbiology

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“…DNA and RNA were extracted from the fractions by the procedure of Schneider (14), measured colorimetricaUy by reactions with twice recrystallized diphenylamine and orcinol respectively. Standard samples of highly polymerized DNA and purified RNA were prepared following the procedure of Kay and Dounce (15,16). RNA was prepared from isolated chromidia of rat liver, DNA from isolated nuclei of thymus tissue of immature rats.…”

Section: Action Of Acth On Nucleic Acidsmentioning

confidence: 99%

The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

Fiala

Sproul

Fiala

1956

The Journal of Cell Biology

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It was shown experimentally that ACTH causes an increase of cytoplasmic RNA and subcellular granules of the adrenal cortex shortly after administration. This effect was found to be concentrated primarily on the basophilic cytoplasmic granules and to be largely independent of cell division. The increase of nuclear RNA, DNA, and nuclear mass followed that of the cytoplasm. The observations indicate also that the mass increase of mitochondrial and chromidial systems is closely related to the increase in cytoplasmic, largely chromidial RNA.

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The Preparation of Sodium Ribonucleate with the Use of Sodium Dodecyl Sulfate¹

Cited by 78 publications

References 4 publications

Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

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The Preparation of Sodium Ribonucleate with the Use of Sodium Dodecyl Sulfate1

Cited by 78 publications

References 4 publications

Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Content and intracellular distribution of the inducing metal in the primary rhabdomyosarcomata induced in the rat by cobalt, nickel and cadmium.

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

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The Preparation of Sodium Ribonucleate with the Use of Sodium Dodecyl Sulfate¹