Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

The Application of Ferritin-Conjugated Antibody to Electron Microscopic Studies of Influenza Virus in Infected Cells

“…incomplete virus, would continue unchanged or even be augmented. Presumably, then, the chorioallantoic fluid would exhibit the maintenance of hemagglutinin titer, diminution in infectivity (11), and reduction in ribonucleic~ acid (13), which characterize the presence of incomplete virus. In this regard it is of interest that Watson and Coons (14), using the technique of immunofluorescence, noted that infected cells undergoing necrosis liberated "specifically stained cell debris."…”

Properties of the Nucleic Acid of the Ryan Strain of Filamentous Influenza Virus

“…The nucleic acid samples were hydrolysed and the purine and pyrimidine derivatives separated by paper chromatography. The separated components were located by their absorption in ultraviolet light, eluted with 0.1 N-HCl and the optical density of the eluate read at the wavelength of maximal absorption for each derivative (Ada & Perry, 1956). The precaution was taken of checking the absorption ratio of each derivative as isolated from the chromatographic paper.…”

“…In two cases, preparations of Ryan Sph. virus were also treated with ribonuclease, the enzyme being added a t the eluate stage and the virus subsequently purified by differential centrifugation (Ada & Perry, 1956). The nucleic acid content of each virus preparation was estimated and the proportion of bases determined.…”

Particle Counts and some Chemical Properties of Murray Valley Encephalitis Virus

“…the digest chromatographed and the purine and pyrimidine derivatives detected and estimated. Details of these procedures were given previously (Ada & Perry, 1956).…”

“…A value of 9200 for E p was assumed (Ada & Perry, 1956). A factor of 10 was assumed for the conversion of the phosphorus value to nucleic acid.…”