The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

Fiala, Silvio; Sproul, Edith E.; Fiala, Anna E.

doi:10.1083/jcb.2.2.115

Cited by 48 publications

(3 citation statements)

References 14 publications

(16 reference statements)

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“…Histological and biochemical studies on patients who had previously undergone unilateral adrenalectomy and on calves indicated that the hypertrophy of the adrenal gland after administration of adrenocorticotrophin (ACTH) was associated with an increase in the concentration of RNA (Symington & Davidson, 1956). Similar changes have been noted in the dog (Bransome & Reddy, 1961), and an increase in the RNA content of the rat adrenal after ACTH administration has been reported by a number of authors (Fiala, Sproul & Fiala, 1956;Povoa & Fontana, 1957; Munro, Hutchison, Ramaiah & Neilson, 1962;Farese & Reddy, 1963). Claims that ACTH also brings about an increase in the DNA content of the gland in immature rats have been made by Fiala et al (1956), and, after a longer period of ACTH treatment, also in mature male rats (Farese & Reddy, 1963).…”

Section: Introductionsupporting

confidence: 54%

The Effect of Adrenocorticotrophin on the Nucleic Acid Metabolism of the Rat Adrenal Gland

Imrie¹,

Ramaiah²,

Antoni³

et al. 1965

Journal of Endocrinology

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Treatment of female rats with adrenocorticotrophin (ACTH) increased the RNA content of the adrenal glands progressively during a period of 3 days, the DNA content increased only after prolonged administration. By contrast, ACTH caused a decrease in the uptake of [32P]orthophosphate into the total RNA of the gland and into most of the RNA fractions of the subcellular components.A method of analysis for RNA and DNA based on the Schmidt\p=m-\Thann-hauser procedure has been evolved which eliminates extraction of nucleic acid by lipid solvents.

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Section: Introductionsupporting

confidence: 54%

The Effect of Adrenocorticotrophin on the Nucleic Acid Metabolism of the Rat Adrenal Gland

Imrie¹,

Ramaiah²,

Antoni³

et al. 1965

Journal of Endocrinology

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“…The administration of ACTH caused a considerable increase in the amounts of all constituents of the gland except DNA (Tables I and 2). An increment in DNA content has, however, been observed after prolonged administration of ACTH to young rats (Fiala, Sproul & Fiala, 1956). The magnitude of the increase in total constituents after ACTH administration was not significantly influenced by the protein or calorie content of the diet (Tables I and 2).…”

Section: Discussionmentioning

confidence: 87%

The influence of diet on the weight and chemical constituents of the rat adrenal gland

et al. 1962

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Although the response of the adrenal gland to excessive intakes of protein has been studied on many occasions (Fahr, 1912; Tepperman, Engel & Long, 1943;Ingle, Ginther & Nezamis, 1943;Ingle, 1945;Benua & Howard, 1945; Leathem, 1945 Leathem, , 1947 Korpbsy, 1952), there are few publications dealing with the effects of protein deficiency on the gland. I n an early series of studies, Limson & Jackson (1932) fed young rats for several months on a diet low in protein which inhibited growth; adrenal weight was reduced by 77,, which was regarded as insignificant. Leathem (1958) found that the adrenal glands of immature rats fed for 30 days on a proteinfree diet were much smaller than the glands of rats fed for a similar period on a diet containing 20 of protein; however, this difference was inverted when the weights of the glands were expressed in relation to the final weights of the rats. In some unpublished experiments, Orton (1937) is reported by Tepperman et al. (1943) to have found no difference in the ratio of adrenal weight to body-weight between growing rats fed ad lib. on a diet containing 3.5% protein and those on a diet containing I 8% protein. When calorie intake on the two diets was restricted to that consumed by the rats on the 3'5% protein diet, those on the 18% protein diet had larger adrenal glands. This experiment suggests that calorie intake may be a significant factor which modifies the response of the adrenal gland to dietary protein intake.These experiments do not provide information about the influence of dietary protein level on individual chemical constituents of the adrenal gland, which may be affected independently of gross changes in gland weight. We have accordingly carried out experiments to determine the effect of complete removal of protein from the diet on the size of the adrenal gland and its content of protein, phospholipid and nucleic acids. Secondly, the possibility that calorie intake modifies the effect of dietary protein content on adrenal function has been examined. Thirdly, we have determined whether the response of the gland to the stimulus of adrenocorticotrophic hormone (ACTH) is affected by the protein and calorie content of the diet. The experiments carried out by previous investigators were confined to growing rats, usually fed on the diets over long periods, and in consequence the interpretation of the action of diet on adrenal weight in these experiments is complicated by large differences in body-weight between groups on different diets. Our experiments were; therefore, carried out on more https://www.cambridge.org/core/terms. https://doi

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“…We have been studying the response of the adrenal cortex to ACTH. After 24 hr of ACTH administration, adrenal cortical RNA content is increased approximately 50% with the primary increase being rRNA (Fíala et al, 1956). Adrenal nuclear RNA polymerase 1 activity has increased by 4 hr and reaches a maximum at 14 hr, while total RNA polymerase II activity remains constant over this time period (Fuhrman and Gill, 1974).…”

mentioning

confidence: 98%

Adrenocorticotropic hormone stimulation of adrenal RNA polymerase I and III activities. Nucleotide incorporation into internal positions and 3' chain termini

Fuhrman¹,

Gill²

1975

Biochemistry

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In the presence of 50 mM (NH4)2SO4 and low concentrations of alpha-amanitin (7.7 mug/ml), adrenal nuclei synthesize predominately rRNA as characterized by size and base composition. Approximately 10% of the RNA synthesized under these conditions sediments at 4-5 S; this RNA synthesizing activity is inhibited by high concentrations of alpha-amanitin (231 mug/ml) indicating the presence of RNA polymerase III activity. ACTH administration to guinea pigs results in a twofold increase in adrenal nuclear RNA polymerase I and III activities at 14 hr of hormone treatment. Analysis of the amount of radiolabeled nucleoside triphosphate incorporated in vitro into 3' chain termini and into internal nucleotide positions has been utilized to measure the number of RNA chains and the average chain length synthesized in vitro. Incorporation into 3' chain termini is not changed by ACTH; incorporation into internal nucleotides is doubled in parallel with the increase in RNA polymerase I activity. These results are not due to an altered Km of RNA polymerase I for the four nucleoside triphosphates, nor to differential R Nase or phosphatase activity. These studies suggest that the regulation of RNA polymerase I by ACTH is accomplished in part through an increase in the rate of RNA chain elongation.

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The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

Cited by 48 publications

References 14 publications

The Effect of Adrenocorticotrophin on the Nucleic Acid Metabolism of the Rat Adrenal Gland

The Effect of Adrenocorticotrophin on the Nucleic Acid Metabolism of the Rat Adrenal Gland

The influence of diet on the weight and chemical constituents of the rat adrenal gland

Adrenocorticotropic hormone stimulation of adrenal RNA polymerase I and III activities. Nucleotide incorporation into internal positions and 3' chain termini

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