The potential application of the protein hydrolysates of three medicinal plants: cytotoxicity and functional properties

Abstract

Functional evaluation of encrypted bioactive peptides in protein structure helps to better understand those for using in pharmacy and food sciences. For this purpose, the total protein was extracted from Matricaria chamomilla, Ziziphora clinopodioides, and Cressa cretica, and partially purified with ammonium sulfate. Protein hydrolysates were obtained from pancreatin hydrolysis for 240 min and the enzyme hydrolysis was confirmed using the determination of hydrolysis degree and Fourier transform infrared (FT-IR) followed by the physicochemical and sensory properties were investigated. The results showed that all hydrolysates had both cytotoxic and antioxidant activities. Specifically, C. cretica hydrolysates represented cytotoxic activity against the MCF-7 cell line with the IC 50 of 135.21 μg/mL, while showed no significant growth inhibition effect on the HEK293 cell line. Besides, M. chamomilla hydrolysates showed the lowest bitterness value (1.125 ± 0.52). From the perspective of color investigation, M. chamomilla hydrolysates indicated the highest L * and the lowest a * factors.The highest turbidity and surface tension,and 10-fold more cancer cell killing effect under gastrointestinal digestion conditions were observed for M. chamomilla hydrolysates. Therefore, bioactive peptides might be formulated in designing of novel anticancer drugs or could be used in promising protocols for the production of food products with beneficial health effects.

Differential effects of oilseed protein hydrolysates in attenuating inflammation in murine macrophages

“…Besides, the surface tension of other oilseed protein hydrolysates ranged from 64.0 mN/m to 66.0 mN/m, much higher compared to dairy and rapeseed protein hydrolysates. Taghizadeh et al (2020) measured surface tension of protein hydrolysates obtained from three medicinal plants (C.retica, Z.clinopodioides and M.chamomilla) after in vitro gastrointestinal digestion with values being much lower than those in our study, ranging from 48.4 mN/m to 59.6 mN/m). In addition, do Evangelho et al (2017) reported the surface tension of pepsin-treated black bean protein hydrolysates to be below 54.0 mN/m.…”

In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates

“…Finally, the absorbance was measured at 570 nm using a microplate reader. The cell viability percentage was calculated by the described equation elsewhere [ 26 ] and IC 50 value was calculated.…”

Experimental, molecular docking and molecular dynamic studies of natural products targeting overexpressed receptors in breast cancer

“…Triton X-100 was used as a positive control. The IC 50 , GI 50 , and LC 50 values were calculated according to the previously described method [ 18 ].…”