Differential effects of oilseed protein hydrolysates in attenuating inflammation in murine macrophages

Han, Ruixian; Hernández-Álvarez, Alan Javier; Maycock, Joanne; Murray, Brent S.; Boesch, Christine

doi:10.1016/j.fbio.2022.101860

Cited by 3 publications

(2 citation statements)

References 57 publications

(55 reference statements)

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“…Amplification of target gene mRNA was conducted using SensiFast SYBR Green reagent using StepOne Real-Time PCR machine (Thermo Fisher) with recommended settings. Primer sequences were designed using NCBI tools, reported in previous research [26,27] and shown in Table 1. Target gene expression was normalized to 𝛽-actin (reference gene) and relative gene expression was calculated using the 2 −ΔΔC T method described by Livak and Schmittgen.…”

Section: Rna Isolation and Quantitative Real-time Pcrmentioning

confidence: 99%

Differential Effects of Betacyanin and Betaxanthin Pigments on Oxidative Stress and Inflammatory Response in Murine Macrophages

Fernando

Sergeeva

Vagkidis

et al. 2023

Molecular Nutrition Food Res

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Scope: Betalain pigments are increasingly highlighted for their bioactive and anti‐inflammatory properties, although research is lacking to demonstrate contributions of individual betalains. The work herein aimed to compare effects of four main betalains on inflammatory and cell‐protective markers and to highlight potential structure‐related relationships of the two main subgroups: betacyanins vs betaxanthins. Methods and results: Murine RAW 264.7 macrophages were stimulated with bacterial lipopolysaccharide following incubation with betacyanins (betanin, neobetanin) and betaxanthins (indicaxanthin, vulgaxanthin I) in concentrations from 1 to 100 µM. All betalains suppressed expression of pro‐inflammatory markers IL‐6, IL‐1β, iNOS, and COX‐2 with tendency for stronger effects of betacyanins compared to betaxanthins. In contrast, HO‐1 and gGCS showed mixed and only moderate induction, while more emphasized effects were observed for betacyanins. While all betalains suppressed mRNA levels of NADPH oxidase 2 (NOX‐2), a superoxide generating enzyme, only betacyanins were able to counteract hydrogen peroxide induced reactive oxygen species (ROS) generation, in alignment with their radical scavenging potential. Furthermore, betaxanthins exerted pro‐oxidant properties, elevating ROS production beyond hydrogen peroxide stimulation. Conclusion: In summary, all betalains display anti‐inflammatory properties, although only betacyanins demonstrate radical scavenging capacities, indicating potential differing responses under oxidative stress conditions, which requires further research.

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Section: Rna Isolation and Quantitative Real-time Pcrmentioning

confidence: 99%

Differential Effects of Betacyanin and Betaxanthin Pigments on Oxidative Stress and Inflammatory Response in Murine Macrophages

Fernando

Sergeeva

Vagkidis

et al. 2023

Molecular Nutrition Food Res

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“…Furthermore, BPs have a wide range of biological activities in the metabolic functions of living organisms and, consequently, in human health, including antimicrobial, antihypertensive, hypoglycaemic activity, immunomodulatory, and antioxidative effects [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Protein Hydrolysates and Bioactive Peptides as Mediators of Blood Glucose—A Systematic Review and Meta-Analysis of Acute and Long-Term Studies

Elbira,

Hafiz,

Hernández-Álvarez

et al. 2024

Nutrients

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Type 2 diabetes mellitus (T2DM) is a major public health concern associated with high mortality and reduced life expectancy. Since diabetes is closely linked with lifestyle, not surprisingly, nutritional intervention and increased physical activity could play a vital role in attenuating the problems related to diabetes. Protein hydrolysates (PHs) and their bioactive peptides (BP) have been shown to exert a wide range of biological effects, including antioxidative, antihypertensive, and in particular, hypoglycaemic activities. To better understand the efficacy of such interventions, a systematic review and meta-analysis of randomised controlled trials (RCTs) were performed concerning the influence of protein hydrolysates on glycaemic biomarkers in subjects with and without hyperglycaemia. Five different databases were used to search for RCTs. In total, 37 RCTs were included in the systematic review and 29 RCTs in the meta-analysis. The meta-analysis revealed a significant reduction in postprandial blood glucose response (PPGR) in normoglycaemic (−0.22 mmol/L; 95% CI −0.43, −0.01; p ≤ 0.05) and in hyperglycaemic adults (−0.88 mmol/L; 95% CI −1.37, −0.39; p ≤ 0.001) compared with the respective control groups. A meta-regression analysis revealed a dose-dependent response for PPGR following PH consumption in normoglycaemic adults, specifically for doses ≤ 30 g. The postprandial blood insulin responses (PPIR) were significantly higher after the ingestion of PHs in both the group with and the group without hyperglycaemia, respectively (23.05 mIU/L; 95% CI 7.53, 38.57; p ≤ 0.01 and 12.57 mIU/L; 95% CI 2.72, 22.41; p ≤ 0.01), compared with controls. In terms of long-term responses, there was a small but significant reduction in both fasting blood glucose (FBG) and fasting glycated haemoglobin (HbA1c) in response to PH compared with the control group (p < 0.05). The PHs significantly improved the parameters of glycaemia in adults and, hence, it may contribute to the management and regulation of the future risk of developing T2DM.

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Chicken slaughterhouse by-products: A source of protein hydrolysates to manage non-communicable diseases

Ibarz-Blanch,

Alcaide-Hidalgo,

Cortés-Espinar

et al. 2023

Trends in Food Science & Technology

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Differential effects of oilseed protein hydrolysates in attenuating inflammation in murine macrophages

Cited by 3 publications

References 57 publications

Differential Effects of Betacyanin and Betaxanthin Pigments on Oxidative Stress and Inflammatory Response in Murine Macrophages

Differential Effects of Betacyanin and Betaxanthin Pigments on Oxidative Stress and Inflammatory Response in Murine Macrophages

Protein Hydrolysates and Bioactive Peptides as Mediators of Blood Glucose—A Systematic Review and Meta-Analysis of Acute and Long-Term Studies

Chicken slaughterhouse by-products: A source of protein hydrolysates to manage non-communicable diseases

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