The physiological and genetic differences between flycatchers (<i>Ficedula albicollis</i> vs. <i>Ficedula hypoleuca</i>)

Drahulian, M. V.; Чаплыгина, А. Б.; Savynska, Nadiia; Kostenko, S.; Ostrovskyi, Pavlo; Gusar, Katerina

doi:10.2478/foecol-2018-0012

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Sources of DNA in birds that are commonly used for genetic analysis can be obtained through non-invasive such as feathers, eggshells, and feces (Begović et al 2017;Kaunisto et al 2017;Maia et al 2017;Drahulian et al 2018;Rytkönen et al 2019;Peters et al 2020;Demarchi et al 2022). In the present study, DNA was obtained from the feather and eggshell material of the M. maleo bird.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing

Samad,

Solihin,

Solihin

et al. 2023

JITV

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The Maleo Senkawor bird (Macrocephalon maleo) is endemic and widely distributed in Sulawesi. Maleo Senkawor bird is monomorphic so it is difficult to distinguish between male and female individuals. This study aimed to determine the kinship relationship among M. maleo in various geographic areas in Sulawesi through the use of the gene marker Cytochrome-b (Cyt-b), determine the sex ratio, and examine the reliability of the 2550F/2718R primer. Fifteen (feather and eggshell) samples from North Sulawesi, Southeast Sulawesi, and Central Sulawesi were collected. Total DNA isolation was performed using the DneasyÂ® Blood and Tissue kit (Cat. No. 69504) following a Qiagen protocol with modification. PCR amplification (35 cycles) used a forward primer MMCytb_F (5'- GAAAATCCCACCCCCTACTA-3'), a reverse primer MMCytb_R (5'- GTTGGCTACGAGGAGTCAGA-3') and a primer for sexing used 2550F/2718R. Analysis was performed on the length of 903 bp Cyt-b mtDNA gene sequences of M. maleo along with their amino acid sequences (301 AA) and Chromo Helicase DNA-Binding (CHD) gene sequences. Based on the genetic distance of the Kimura 2-Parameter model and p-distance, the interpopulation of M. maleo in Sulawesi (the population of Southeast Sulawesi was separated from the population of Central Sulawesi and North Sulawesi) was 0.002 (0.2%) â€“ 0.003 (0.3%). Each population has formed a different haplotype. Primer 2550F/2718R was able to amplify the CHD gene and could distinguish the sex identification. Male individuals are more dominant than female individuals with a sex ratio of 6,5:1

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Section: Introductionmentioning

confidence: 99%

Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing

Samad,

Solihin,

Solihin

et al. 2023

JITV

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“…The birds simply do not depend on a particular species of insects (Litt et al 2014, Schirmel et al 2016, Hejda et al 2017. Some studies have identified the role of feed for sympatric species (Drahulian et al 2018) and specific features of overlapping trophic niches of different birds (Lagar 2016).…”

Section: Introductionmentioning

confidence: 99%

Invertebrates in the diet of collared flycatcher (Ficedula albicollis) nestlings in transformed forest ecosystems of north-eastern Ukraine

Чаплыгина

Yuzyk²,

Savynska³

et al. 2022

BALT FOR

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The diet composition of collared flycatcher nestlings, Ficedula albicollis (Temminck, 1815), in the transformed forests of northeastern Ukraine was investigated to present an argument for conservation management actions that could benefit the species. Food pellets were collected from nestlings in four study sites with differing levels of recreational disturbance over 10 breeding seasons. The invertebrates they contained were identified, divided into trophic groups and compared across sites. A total of 1,160 food pellets were collected, containing 3,253 invertebrates from 294 species. Insects comprised 83% of the prey, with the most abundant orders of Diptera, Coleoptera and Lepidoptera. The smallest diversity of prey species was found in samples from the most disturbed site. The qualitative composition of the prey items showed that zoophages were the most frequent trophic group encountered, followed by phytophages, saprophages, polyphages, and necrophages. However, the order of trophic group frequency varied between sites. Although collared flycatchers forage within a limited area, their diet is characterized by a wide diversity of prey, and they exploit all forest layers. Key words: collared flycatcher, diet, trophic links, zoophages, phytophages, saprophages, necrophages, polyphages

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Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

Rudaуа

Katerynych

Drahulian³

et al. 2020

Regul. Mech. Biosyst.

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This article presents an elaboration of the protocol for the method of sexing wild birds based on the polymorphism of the CHD gene using P2/P8 primer for Common Pheasant – Phasianus colchicus (Linnaeus, 1758; Galliformes, Phasianidae); Silver Lofur or Silver Pheasant – Lophura nycthemera (Linnaeus, 1758; Galliformes, Phasianidae), Budgerigar – Melopsittacus undulatus (Shaw, 1805; Psittaciformes, Psittacidae), Herring Gull – Larus argentatus (Pontoppidan, 1763; Charadriiformes, Laridae), and White Stork – Ciconia ciconia (Linnaeus, 1758; Ciconiiformes, Ciconiidae). Blood samples were taken from Common Pheasant, Silver Pheasant and White Stork using the “drop of blood on paper” method. For the Budgerigar and the Herring Gull, DNA was isolated from the feather follicle. To isolate DNA, a commercial NeoPrep 100 DNA reagent kit (Neogen, Ukraine) was used. Primers P2/P8 were used for PCR; PCR was performed using GenPac PCR Core reagents (Neogen, Ukraine). We selected the optimal amount of Tag polymerase, the amount of DNA and primers and, according to the amount of reagents, set acceptable amplification modes and electrophoresis agarose gel percentage. Prior to PCR, additional DNA gel electrophoresis purification is proposed, which increases the percentage of positive sex determination results. It was found that the ideal mixture for the 5 bird species was an amplification mixture (total volume 20 µL, containing 1 U Tag polymerase, 100 ng DNA and 0.6 µM of each primer). The amplified CHD-Z fragment of Common and Silver pheasants is of ~340 n. p., CHD-W ~360 n. p. Herring Gull and Budgerigar have ~350 n. p. of CHD-Z length, and ~400 n. p. of CHD-W length, White Stork has its CHD-Z of ~ 370 n. p. long. It is advisable to investigate the genome of the experimental bird species using horizontal electrophoresis in agar’s gel with the concentration of 5%, which makes it possible to clearly visualize the female genotype. The universal protocol of the method of sex determination based on polymorphism of the CHD gene for the 5 studied bird species is described. These results of the study led to the conclusion that for the simultaneous sexing of several species of birds, it is advisable to develop a unified protocol for determining the status of the CHD gene, with the aim of clarifying the gender, as well as new approaches in ornithology and ecology aimed at determining interspecific differences associated with gene polymorphism. Identification of differences in fragment sizes may be useful for identifying the species in cases when birds form mixed pairings for taxonomic and phylogenetic comparisons.

show abstract

The physiological and genetic differences between flycatchers (Ficedula albicollis vs. Ficedula hypoleuca)

Cited by 3 publications

References 46 publications

Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing

Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing

Invertebrates in the diet of collared flycatcher (Ficedula albicollis) nestlings in transformed forest ecosystems of north-eastern Ukraine

Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

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