The Measurements Of The Cholinesterase Activity Of Brain And Plasma In Rabbits By Using Modified Michel And Ellman Assays

Abstract

This study deals with the measurement of acetylcholinesterase activities in the plasma and brain of 8 rabbits using modified Michael and Ellman methods. The highest values of acetylcholinesterase activities in plasma and brain were 0.37 and 0.25, respectively, when the modified Michel method was employed. In the case of using Ellman method, the highest values of cholinesterase activities in plasma and brain were 0.89 and 0.43, respectively. In order to determine the efficiency and legitimacy of the measured result, the coefficient of variation was calculated. For the modified Michael method, the coefficients of variation in plasma and brain were 17.78 % and 19.37%, respectively. However, in the case of using Ellman method, the coefficients of variation in plasma and brain were 21.72% and 41.21%, respectively. These results were consistent with those reported in the previous studies.

Study of the biochemical, histological and cytogenetic effects of two different energy drinks (EDs); Red bull and power horse; on brain of adult male Albino rats and to determine the possible protective role of omega-3 on the adverse effects of EDs".

“…Acetylcholinesterase enzyme has very vital role in decreasing the neurotransmitter acetylcholine level and converting it to acetic acid and choline [35]. This enzyme is widely distributed throughout the body in both neuronal and non-neuronal tissues.…”

Cell membrane enveloped polymeric nanosponge for detoxification of chlorpyrifos poison: In vitro and in vivo studies

“…E-BC-K174, Elabscience ® , USA) based on an improved method. 17,18 The assay principle is an optimized version of Ellman method. The thiocholine produced by AChE enzyme from acetylcholine reacts with 5,5′-dithiobis (2-nitrobenzoic acid) to form a yellow-coloured product, 5-mercapto-nitrobenzoic acid (TNB).…”

Chemical composition and the inhibition of acetyl cholinesterase activity of Centella asiatica from Quang Tho village, Quang Dien district, Thua Thien – Hue province

“…Fr.CAM 7.11 (8 g, 2.28 % of dry raw material) contained asiaticoside A and asiaticoside B as a mixture with a ratio of 65:35, which could not be separated by column chromatography. [16][17][18][19] The assay was carried out in the Institute for Biotechnology, VAST. 20 mg mice brain (white Swiss BALBC mice) was homogenized with 4 ml phosphate buffer.…”