The lipid metabolism alteration of three spirocyclic tetramic acids on zebrafish (Danio rerio) embryos

Zhang, Jie; Qian, Le; Teng, Miaomiao; Mu, Xiyan; Qi, Suzhen; Chen, Xiangguang; Zhou, Yanmin; Cheng, Yi; Pang, Sen; Li, Xuefeng; Wang, Chengju

doi:10.1016/j.envpol.2019.02.035

Cited by 28 publications

(10 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LPL and FASN activity assays were determined using enzyme-linked immunosorbent assay (ELISA) kits (Fu life Industry Co., Ltd. Shanghai, China). 38 The range suitabilities of LPL and FASN were 1.6−65 U/mL and 10−360 U/L, respectively. The standard calibrations of LPL and FASN were shown in Figure S2.…”

Section: ■ Materials and Methodsmentioning

confidence: 93%

See 1 more Smart Citation

Effect of Propiconazole on the Lipid Metabolism of Zebrafish Embryos (Danio rerio)

Teng

Zhao

Zhou

et al. 2019

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Propiconazole is a triazole fungicide that has been widely used in agriculture and has been detected in the aquatic environment. This study aimed to investigate the effects of propiconazole exposure on lipid metabolism in the early life stages of zebrafish for 120 h postfertilization (hpf). Using the early life stages of zebrafish to address scientific questions is lower in cost, more efficient, and suitable to meeting current legislation than those in other traditional fish species. Exposure to propiconazole significantly inhibited the development of zebrafish embryos and larvae. This exposure also caused reduced locomotor activities in zebrafish. Furthermore, total cholesterol levels, lipoprotein lipase, and fatty acid synthase activities were significantly decreased. The expression levels of genes involved in lipid metabolism were significantly up-regulated in response to propiconazole exposure. GC-MS/MS analysis revealed that fatty acids were significantly decreased. Together, the findings indicate the potential environmental risks of propiconazole exposure in the aquatic ecosystem.

show abstract

Section: ■ Materials and Methodsmentioning

confidence: 93%

“…The supernatant was used for enzymatic activity analysis. The LPL and FASN activity assays were determined using enzyme-linked immunosorbent assay (ELISA) kits (Fu life Industry Co., Ltd. Shanghai, China) . The range suitabilities of LPL and FASN were 1.6–65 U/mL and 10–360 U/L, respectively.…”

Section: Methodsmentioning

confidence: 99%

Effect of Propiconazole on the Lipid Metabolism of Zebrafish Embryos (Danio rerio)

Teng

Zhao

Zhou

et al. 2019

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum biochemistry parameters of fish species are very important factors to assess the nutritional and health status of the organism (Zhang et al., 2016). TCHO and TG concentration are important biomarkers, indicating alterations in lipid metabolism (Zhang, Qian, et al, 2019). HDLC, synthesized mainly in the liver and the small intestine, plays important roles in eliminating cholesterol in serum (Jiajun, Aiyun, Shanshan, & Minhong, 2011; Saleh Gazwi & Mahmoud, 2019).…”

Section: Discussionmentioning

confidence: 99%

Effects of ferulic acid on growth performance, immunity and antioxidant status in genetically improved farmed tilapia ( Oreochromis niloticus ) fed oxidized fish oil

Huang

Jiang

et al. 2020

Aquacult. Nutr.

View full text Add to dashboard Cite

A study was conducted to characterize the effects of dietary oxidized fish oil on the growth performance, immunity and antioxidant status of genetically improved farmed tilapia (Oreochromis niloticus) and to determine the role of ferulic acid on the oxidative damage induced by the oxidized fish oil. The tilapia (13.73 ± 0.31 g) were fed four experimental diets containing untreated (peroxide value, POV: 2.2 meq/kg) and highly oxidized (POV: 120.6 meq/kg) fish oil either with or without ferulic acid (0 or 400 mg/kg) supplementation for 12 weeks. From the results, the oxidized fish oil treatments increased antioxidant enzyme activities and MDA values but decreased the weight gain and the immunological parameters in tilapia. Meanwhile, the serum biochemical indices were significantly affected by the oxidized fish oil. Besides, the addition of ferulic acid partially counteracted the free radical‐induced damage and improved the health status of tilapia. In conclusion, the oxidized fish oil may induce oxidative stress, destroy liver, dysregulate lipid metabolism as well as reduce non‐specific immunity, and eventually result in growth inhibition of tilapia. The ferulic acid supplementation partially offset the negative effects of the oxidized fish oil on tilapia.

show abstract

“…This compound also causes biochemical, histopathological, and physiological changes in the ovaries of adult zebrafish (Danio rerio) (Wu et al, 2018). In zebrafish embryos, it is a teratogen agent (Zhang et al, 2019), affects lipid metabolism and causes mitochondrial lesions (Zhang et al, 2020). Spirodiclofen is considered to be a risk factor for adrenal cortical vacillation, atrophy of male reproductive tract Leydig cell hyperplasia and uterine adenocarcinoma in rats (Yoshida et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Spirodiclofen is considered to be a risk factor for adrenal cortical vacillation, atrophy of male reproductive tract Leydig cell hyperplasia and uterine adenocarcinoma in rats (Yoshida et al, 2015). Spirodiclofen was monitored in human Caco-2 cells (Shi et al, 2017) and in human serum (Shin et al, 2018) and produces defects in zebrafish embryonic development (Zhang et al, 2019). Toxicity tests with different model organisms play an important role in evaluating environmental risk and in classifying the hazardous effects of pesticides on human health.…”

Section: Introductionmentioning

confidence: 99%

Movento® 240SC (Spirotetramat) and Envidor® 240SC (Spirodiclofen) keto-enol insecticides induce DNA damage in <i>Drosophila melanogaster</i> ovaries

González-Marín

Calderón-Segura

Pérez

et al. 2021

Fundam. Toxicol. Sci.

View full text Add to dashboard Cite

Movento® 240SC and Envidor® 240SC are new insecticide derivatives of tetramic acid belonging to a keto-enol pesticide family. However, few studies have reported genotoxic effects in nontarget organisms. In the present study, the genotoxic effects of Movento® 240SC and Envidor® 240SC on Drosophila melanogaster ovaries were analyzed using the alkaline comet assay. Simultaneously, we determined the LD 50 for both insecticides. Virgin females were exposed to food at three sublethal concentrations (11.2, 22.4, 37.3 mg/L) of Movento® 240SC and (12.3, 24.6, 41.1 mg/L) of Envidor® 240SC for 72 hr. As a negative control group, females were exposed to food without insecticides, and as a positive control group, females were exposed to 17.5 mg/L bleomycin under the same experimental conditions. We analyzed three genotoxic parameters, tail length, tail moment, and tail intensity, in ovarian cells. The results showed that 11.2 mg/L Movento® 240SC insecticide significantly increased the tail intensity mean in ovarian cells compared with the negative control. However, 22.4 and 37.3 mg/L Movento® 240SC significantly increased tail length and tail moment means compared with the negative control. Envidor® 240SC insecticide at 12.3, 24.6, 41.1 mg/L significantly increased the three genotoxic parameters in ovarian cells compared with the negative control. The LD 50 values of Movento® 240SC and Envidor® 240SC insecticides were 79.1 mg/L and 78.0 mg/L, respectively. The genotoxic response of the two keto-enol pesticides was dependent on the concentration of each pesticide. The results demonstrated that Movento® 240SC and Envidor® 240SC keto-enol insecticides are genotoxic agents in D. melanogaster ovaries.

show abstract

The lipid metabolism alteration of three spirocyclic tetramic acids on zebrafish (Danio rerio) embryos

Cited by 28 publications

References 74 publications

Effect of Propiconazole on the Lipid Metabolism of Zebrafish Embryos (Danio rerio)

Effect of Propiconazole on the Lipid Metabolism of Zebrafish Embryos (Danio rerio)

Effects of ferulic acid on growth performance, immunity and antioxidant status in genetically improved farmed tilapia ( Oreochromis niloticus ) fed oxidized fish oil

Movento® 240SC (Spirotetramat) and Envidor® 240SC (Spirodiclofen) keto-enol insecticides induce DNA damage in <i>Drosophila melanogaster</i> ovaries

Contact Info

Product

Resources

About