The investıgatıon of GSTT1, GSTM1 and SOD polymorphism in bladder cancer patıents

Kılıç

Clinical Laboratory Analysis

et al. 2010

It is well known that antioxidants and reactive oxygen species play an important role in carcinogenesis. In this study, we attempted to evaluate antioxidant enzyme activities and lipid peroxidation levels in cancerous bladder tissue and to determine their relationship with bacterial infection. Bacterial culture was made from all urine samples using Blood and Eosin Methylene Blue agars for checking the presence of bacterial infections. We measured thiobarbituric acid reactive substances (TBARs) and activities of xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in cancerous tissues of 25 bladder cancer patients, in noncancerous adjacent bladder tissues of 13 out of these 25 patients, and in control bladder tissues of 15 patients with a non-neoplastic genitourinary disease. TBARs levels increased and XO, SOD, GSH-PX, and CAT activities decreased significantly in cancerous bladder tissues. TBARS, XO, and SOD levels were not significantly different between noncancerous adjacent tissue and control bladder tissue. Statistically significantly lower GSH-PX and higher CAT activities were observed in noncancerous adjacent bladder tissue compared with cancerous tissue. GSH-PX level of tumor tissue was correlated significantly with tumor grade (r=-0.425, P=0.034). Results suggested that pathway activity of free radicals were accelerated in the cancerous human bladder tissues via increased TBARs levels and decreased enzyme activities of XO, SOD, GSH-PX, and CAT, which implicated a severe exposure of cancerous tissues to oxidative stress.

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Lipid peroxidation and antioxidant enzyme activities in cancerous bladder tissue and their relation with bacterial infection: a controlled clinical study

Kılıç

Clinical Laboratory Analysis

et al. 2010

Asian Pacific Journal of Cancer Prevention

“…We did not observe an increased BC risk between null and wild types of GSTM1 gene. In previous studies, while some investigators reported an association between null genotype and overall BC risk (Toruner et al, 2001;Cengiz et al, 2007), another did not (Altayli et al, 2009). GSTT1 null genotype was reported to be associated with increased BC risk among smokers in Turkish population (Altayli et al, 2009).…”

Section: Discussionmentioning

confidence: 93%

CYP1A1 (Ile⁴⁶²Val), CYP1B1 (Ala¹¹⁹Ser and Val⁴³²Leu), GSTM1 (null), and GSTT1 (null) Polymorphisms and Bladder Cancer Risk in a Turkish Population

Berber

Yılmaz²,

Yılmaz³

et al. 2013

We aimed to investigate bladder cancer risk with reference to polymorphic variants of cytochrome p450 (CYP) 1A1, CYP1B1, glutathione S-transferase (GST) M1, and GSTT1 genes in a case control study. Polymorphisms were examined in 114 bladder cancer patients and 114 age and sex-matched cancer-free subjects. Genotypes were determined using allele specific PCR for CYP1A1 and CYP1B1 genes, and by multiplex PCR and melting curve analysis for GSTM1 and GSTT1 genes. Our results revealed a statistically significant increased bladder cancer risk for GSTT1 null genotype carriers with an odds ratio of 3.06 (95% confidence interval=1.39-6.74, p=0.006). Differences of CYP1A1, CYP1B1 and GSTM1 genotype frequencies were not statistically significant between patients and controls. However, the specific combination of GSTM1 null, GSTT1 null, and CYP1B1 codon 119 risk allele carriers and specific combination of GSTM1 present, GSTT1 null, and CYP1B1 432 risk allele carriers exhibited increased cancer risk in the combined analysis. We did not observe any association between different genotype groups and prognostic tumor characteristics of bladder cancer. Our results indicate that inherited absence of GSTT1 gene may be associated with bladder cancer susceptibility, and specific combinations of GSTM1, GSTT1 and CYP1B1 gene polymorphisms may modify bladder cancer risk in the Turkish population, without any association being observed for CYP1A1 gene polymorphism and bladder cancer risk.

“…Lightfoot et al also reported decreased incidence of non-Hodgkin's lymphoma in homozygotes for the SOD2 16Ala allele in the UK population (27). Additionally, studies on bladder cancer were unable to indicate a positive correlation between polymorphisms within SOD gene and the occurrence of this form of cancer (32,33). According to the results presented by our study and other investigators, it may be concluded that the correlation between SOD2 polymorphisms is dependent on the kinds of cancers, for example, prostate and breast cancers appear to a have positive correlation, while, bladder and hematological malignancies, like lymphoproliferative disorders, are not associated with the polymorphisms of SOD2.…”

Section: Discussionmentioning

confidence: 99%

Lack of Association Between Superoxide Dismutase Gene Polymorphism and Malignant Lymphoproliferative Disorders

et al. 2016

Background: Superoxide dismutase is one of the most important antioxidant enzymes that protect cells against destructive effects of superoxide anion. Objectives: The aim of this study was to evaluate the association between the C47T polymorphism (rs4880) of the manganese superoxide dismutase (Mn-SOD) gene and the risk of malignant lymphoproliferative disorders (MLDs). Patients and Methods: Manganese superoxide dismutase polymorphism was genotyped using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) in 103 patients with MLDs and 103 healthy control subjects. Results: The frequencies of the CC, CT and TT genotypes were 29.1%, 51.5% and 19.4%, respectively, in patients with MLDs and 24.3%, 47.6% and 28.2%, respectively, in the control group. There were no statistical differences in the genotype or allele frequency of rs4880 between cases and controls. Conclusions: According to the fact that Mn-SOD gene polymorphisms have been considered as a major risk factor in some malignancies, this single center study did not find any association between the rs4880 polymorphism of the manganese superoxide dismutase gene and risk of MLDs.