Lipid peroxidation and antioxidant enzyme activities in cancerous bladder tissue and their relation with bacterial infection: a controlled clinical study

Abstract

It is well known that antioxidants and reactive oxygen species play an important role in carcinogenesis. In this study, we attempted to evaluate antioxidant enzyme activities and lipid peroxidation levels in cancerous bladder tissue and to determine their relationship with bacterial infection. Bacterial culture was made from all urine samples using Blood and Eosin Methylene Blue agars for checking the presence of bacterial infections. We measured thiobarbituric acid reactive substances (TBARs) and activities of xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in cancerous tissues of 25 bladder cancer patients, in noncancerous adjacent bladder tissues of 13 out of these 25 patients, and in control bladder tissues of 15 patients with a non-neoplastic genitourinary disease. TBARs levels increased and XO, SOD, GSH-PX, and CAT activities decreased significantly in cancerous bladder tissues. TBARS, XO, and SOD levels were not significantly different between noncancerous adjacent tissue and control bladder tissue. Statistically significantly lower GSH-PX and higher CAT activities were observed in noncancerous adjacent bladder tissue compared with cancerous tissue. GSH-PX level of tumor tissue was correlated significantly with tumor grade (r=-0.425, P=0.034). Results suggested that pathway activity of free radicals were accelerated in the cancerous human bladder tissues via increased TBARs levels and decreased enzyme activities of XO, SOD, GSH-PX, and CAT, which implicated a severe exposure of cancerous tissues to oxidative stress.

Evaluation of oxidative status in patients with <i>Fasciola hepatica</i> infection

“…In some diseases, an increase in reactive oxygen species is not the primary cause of the illness; it develops secondarily to the primary disorder and is involved in pathogenesis 14 . There are some studies indicating that oxidative stres plays a role in the pathogenesis of viral hepatitis, sepsis, and bacterial infections [15][16][17][18][19] . Numerous studies demonstrated that in many infectious diseases, a variety of inflammatory cells are activated, which lead to production of reactive oxygen and nitrogen species to kill parasites.…”

U83836E Inhibits Retinal Neurodegeneration in Early-Stage Streptozotocin-Induced Diabetic Rats

“…This aliquot was spectrophotometrically analyzed, as described by Bayraktar et al [18] . Briefly, xanthine-xanthine oxidase was used to generate a superoxide flux.…”

Catalase, carbonic anhydrase and xanthine oxidase activities in patients with mycosis fungoides

“…CAT prevents the accumulation of H 2 O 2 by catalyzing the rapid decomposition of H 2 O 2 to O 2 and H 2 O and it is one of the most important antioxidant enzymes. Although there are several reports that CAT activity is suppressed in cancerous tissues [22][23][24][25] , some authors found unchanged or increased activity in some tumor tissues 26,27 . Durak et al 22 found decreased CAT activity in cancerous bladder tissues.…”