The Influence Of Age Upon The Hexosamine-Collagen Ratio Of Dermal Biopsies From Men

Action of Testosterone on the Skin of Aging Male Subjects

“…This was confirmed and extended by Lorenzen and Za charies [6], Because of the previous observations th at the aging process results in a gradual reduction of the mucopolysaccharides of the skin as measured by the hexosamine-collagen ratio [7], the present study was undertaken. It was proposed to determine if a potent long-acting preparation of testosterone would either arrest the tendency to a decreased hexosamine content of skin or actually reverse the process resulting in an increased hexosamine content with a resulting increase in the hexosamine-collagen ratio of the skin.…”

“…[7] found a gradual decrease in the hexosamine-collagen ratio (H/C ratio) with aging. This study indicated th at as age increases there is a decrease in the ground substance of the dermis in relation to collagen.…”

“…[7] with the exception that the actual analyses were autom ated* * * , while the other set was fixed in 10% formalin, imbedded and cut into 4 p sections. These were stained with hematoxylin and cosin, acid orcein-Gicmsa stain, Wilder's reticulum stain, Alcian blue and periodic acid-Schiflf reaction (PAS), as described in " Manual of Histologic and Special Staining Technics" , Armed Forces Institute of Pathology, 2d Kdition (The Blakiston Division, McGraw-Hill Books Co.).…”

Aging of Connective Tissue and Molecular Transport

“…Let Rt = R0eat d" Assume R = cr Ft Rt = age specific mortality rate Ft = molecular flow factor which decreases with age According to Fick's first law of diffusion [Sobel] a) Which could affect diffusion coefficient 1. Hcxosamine/collagen [92] 2. Hexosaminc/collagen [76] 3.…”

The Characteristics of Newly Formed Collagen during Ageing

“…Since the total amount of hexosamine does H r u z a and H lavaekovS, The Characteristics of not change with age the extractability of hexosamine decreases. The hexosamine/hydroxyproline ratio, on other hand, decreases only before full maturity is attained, while Sobel et al (1958) and KungYing et al (1960) state that this fall occurs throughout life. Age changes are characterized much more exactly by the hexosamine/ hydroxyproline ratio in the nonextractable remnant, where a regular decrease with age is seen .…”