The in vitro antioxidant properties of alcalase hydrolysate prepared from silkie fowl (Gallus gallus) blood protein

Abstract

Two types of proteins including blood plasma protein and blood cell protein were isolated from silkie fowl (Gallus gallus) blood and hydrolyzed using alcalase for 0, 2, 4 and 6 h. The blood plasma protein hydrolysate (BPH) and blood cell protein hydrolysate (BCH) were analyzed for pH value, peptide content and antioxidative properties. The significantly higher peptide contents were observed in BPH than that of BCH, which showed that blood plasma protein was more suitable to hydrolysis by alcalase than blood cell protein. Both BPH and BCH showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and Fe(2+) chelating ability. BPH at 4 h of hydrolysis (BPH4) demonstrated significantly higher antioxidant capacity than those treated by alcalase in most of the assays. The BPH4 was separated using ultra-filtration and assessment of the fractions and indicated that low molecular weight of peptides (< 3 kDa) possessed greater DPPH scavenging activity, Fe(2+) chelating ability and inhibitory activity of lipid peroxidation. These results show that BPH has the potential to be ingredients in the food industry as a replacement of synthetic antioxidants.

Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells

“…One previous report also showed that low-molecular peptides (941.43 Da) from duck skin by-products have high radical scavenging activities and protect the liver against oxidative damage. In the previous studies, the 0-3 kDa of Monkfish muscle proteins hydrolysate (MPTH-I), Blood plasma hydrolysate (BPH4) and peptides derived from biodegradation of chicken feather (PBCF) showed the highest antioxidant activities than other molecular weights, corroborating our result that LMWSPs (0-3 kDa) have highest antioxidant activities (Hu et al 2020a, b;Cheng et al 2016;Alahyaribeika et al 2021). Table 1 shows that the difference of antioxidant activities between peptides (0-3 kDa) derived from four different species.…”

Investigating the cellular antioxidant and anti-inflammatory effects of the novel peptides in lingzhi mushrooms

“…One earlier study revealed that peptides of low molecular weight (941.43 Da) derived from the by-products of duck skin are effective free radical scavengers and also prevent oxidative damage to the liver [ 62 ]. In the earlier works, it was found that the 0–3 kDa of monkfish muscle protein hydrolysate (MPTH-I) [ 63 ], blood plasma hydrolysate (BPH4) [ 64 ], and peptides obtained from biodegraded chicken feathers (PBCF) [ 65 ] achieved greater levels of antioxidant activity when compared to other molecular weights, offering support to our finding that low molecular weight produce the greatest antioxidant activity.…”

Hypolipidemic and anti‐inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model

“…However, animal by‐products as a good nutrient source is particularly rich in protein and bioactive products have recently been investigated (Cheng et al . , ; Jayathilakan et al . ).…”