Hypolipidemic and anti‐inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model

Чернуха, И. М.; Fedulova, Liliya; Kotenkova, Elena; Takeda, Shiro; Sakata, Ryoichi

doi:10.1111/asj.12986

Cited by 16 publications

(12 citation statements)

References 34 publications

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“…Hyperlipidemia is characterized by an increase in triglyceride (TG), total cholesterol (TC), and low-density lipoprotein-cholesterol (LDL-c) and/or a decrease in high-density lipoprotein-cholesterol (HDL-C) [ 5 ]. Increasing evidence has shown that this abnormality in lipid metabolism causes lipid accumulation, oxidative stress, and inflammation, leading to cardiac damage [ 6 , 7 ]. Several researchers have investigated various drugs for the treatment of hyperlipidemia, such as statins; however, as these drugs are related to the development of cell resistance and are associated with adverse effects, new methods for treating hyperlipidemia are needed.…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Pei

Guo

Zhu

et al. 2020

BioMed Research International

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Hyperlipidemia is a risk factor for cardiac damage and cardiovascular disease. Increasing evidence has shown that dyslipidemia-related cardiac damage is associated with lipid accumulation, oxidative stress, and inflammation. Thymoquinone (TQ) is the major constituent of Nigella sativa, commonly known as black seed or black cumin, and is globally used in folk (herbal) medicine for treating and preventing a number of diseases and conditions. Several studies have shown that TQ can protect against cardiac damage. This study is aimed at investigating the possible protective effects of TQ on hyperlipidemia-induced cardiac damage in low-density lipoprotein receptor-deficient (LDL-R-/-) mice. Eight-week-old male LDL-R-/- mice were randomly divided into normal diet (ND), high-fat diet (HFD), and HFD and TQ (HFD+TQ) groups and were fed the different diets for eight weeks. Blood samples were obtained from the inferior vena cava in serum tubes and stored at -80°C until use. Some cardiac tissues were fixed in 10% formalin and then embedded in paraffin for histological evaluation. The remainder of the cardiac tissues was snap-frozen in liquid nitrogen for mRNA preparation or immunoblotting. The levels of metabolism-related factors, such as total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-c), and high-sensitivity C-reactive protein (hs-CRP), were decreased in the HFD+TQ group compared with those in the HFD group. Periodic acid-Schiff staining demonstrated that lipid deposition was lower in the HFD+TQ group than in the HFD group. The expression of pyroptosis indicators (NOD-like receptor 3 (NLRP3), interleukin- (IL-) 1β, IL-18, and caspase-1), proinflammatory factors (IL-6 and tumor necrosis factor alpha (TNF-α)), and macrophage markers (cluster of differentiation (CD) 68) was significantly downregulated in the HFD+TQ group compared with that in the HFD group. Our results indicate that TQ may serve as a potential therapeutic agent for hyperlipidemia-induced cardiac damage.

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Section: Introductionmentioning

confidence: 99%

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Pei

Guo

Zhu

et al. 2020

BioMed Research International

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“…Furthermore, the high plasma level of the atherogenic index is related to small LDL-cholesterol particles. This is a predictor of conditions such as obesity, insulin resistance and inflammation, and consequently coronary artery disease, diabetes mellitus, and metabolic syndrome [48,49].…”

Section: Discussionmentioning

confidence: 99%

Use of an Extract of Annona muricata Linn to Prevent High-Fat Diet Induced Metabolic Disorders in C57BL/6 Mice

et al. 2019

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Annona muricata Linn, commonly known as graviola, is one of the most popular plants used in Brazil for weight loss. The aim of this study is to evaluate the therapeutic effects of three different doses (50 mg/kg, 100 mg/kg, and 150 mg/kg) of aqueous graviola leaf extract (AGE) supplemented by oral gavage, on obese C57BL/6 mice. Food intake, body weight, an oral glucose tolerance test (OGTT), an insulin sensitivity test, quantification of adipose tissue cytokines, weight of fat pads, and serum biochemical and histological analyses of the liver, pancreas, and epididymal adipose tissue were measured. AGE had an anti-inflammatory effect by increasing IL-10 at doses of 50 and 100 mg/kg. Regarding the cholesterol profile, there was a significant decrease in LDL-cholesterol levels in the AGE 150 group, and VLDL-cholesterol and triglycerides in the AGE 100 and 150 groups. There was an increase in HDL cholesterol in the AGE 150 group. The extract was able to reduce the adipocyte area of the epididymal adipose tissue in the AGE 100 and 150 groups. According to the histological analysis of the liver and pancreas, no significant difference was found among the groups. There were no significant effects of AGE on OGTT and serum fasting glucose concentration. However, the extract was effective in improving glucose tolerance in the AGE 150 group.

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“…All rats were kept under 12-h light-dark cycles at (23±1)°C and (50±5)% humidity, and had free access to food and water. An atherosclerotic rat model was established through endothelial injury of the iliac arteries and feeding with a 2.5% cholesterol diet with 1% glucose (Sigma-Aldrich, Merck KGaA) for 6 weeks as described previously (19). The rats were randomly divided into two experimental groups: i) The control group that received PBS treatment orally and ii) the experimental group that orally received Pts (≥99%, purity; 10 mg/kg/day; Great Forest Biomedical, Ltd.) treatment with a regular diet for 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

Pterostilbene reduces endothelial cell injury in vascular arterial walls by regulating the Nrf2‑mediated AMPK/STAT3 pathway in an atherosclerosis rat model

Tang

Duan

et al. 2019

Exp Ther Med

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Endothelial cell injury in vascular arterial walls is a hallmark of atherosclerosis. Pterostilbene (Pts) has been shown to have an anti-oxidative and anti-apoptotic effect in numerous diseases via regulation of intracellular metabolism. The purpose of this study was to investigate the protective effect and possible mechanism of Pts against endothelial cell apoptosis in an atherosclerotic rat model. An atherosclerotic rat model was established using a high-fat, high glucose and high cholesterol diet. The effects of Pts on apoptosis and oxidative stress injury were measured using atherosclerotic lesion analysis, western blot analysis, hematoxylin and eosin straining, TUNEL assay and immunohistochemistry. In vivo results in an atherosclerosis rat model showed that Pts administration decreased the inflammatory response. Pts administration attenuated atherogenesis, reduced aortic plaque size, reduced macrophage infiltration, and suppressed oxidative stress and apoptosis of vascular arterial walls. In vitro assays using cultured human endothelial cells showed that Pts administration decreased hydrogen peroxide-induced cytotoxicity, oxidative stress injury and apoptosis via nuclear factor erythroid 2-related factor 2 (Nrf2) activation in endothelial cells. Additionally, Pts administration increased the expression level of Nrf2 and 5′ adenosine monophosphate-activated protein kinase (AMPK), and the phosphorylation level of AMPK and decreased signal transducer and activator of transcription 3 (STAT3) expression in these cells. Furthermore, knockdown of Nrf2 prevented Pts-decrease oxidative stress injury and apoptosis. In conclusion, these data suggest that Pts can protect endothelial cells in the vascular arterial walls against atherosclerosis-induced injury through regulation of the Nrf2-mediated AMPK/STAT3 pathway.

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Hypolipidemic and anti‐inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model

Cited by 16 publications

References 34 publications

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Use of an Extract of Annona muricata Linn to Prevent High-Fat Diet Induced Metabolic Disorders in C57BL/6 Mice

Pterostilbene reduces endothelial cell injury in vascular arterial walls by regulating the Nrf2‑mediated AMPK/STAT3 pathway in an atherosclerosis rat model

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