The oil processing industry generates significant quantities of lemon basil seed residue which is not currently used to any great extent. However, this by-product has important potential as a source...
Defatted rice bran (DRB) is a by-product of oil extraction from rice bran (Oryza sativa L.), and has health benefits. This in vitro study investigated the antioxidant and anti-inflammatory effects of four crude DRB protein hydrolysate fractions prepared by pepsin-pancreatin digestion and fractionated by ultrafiltration on a transformed macrophage cell line, RAW264.7. Protein hydrolysates with MW <3 kDa (F4 fraction) had the lowest IC 50 for NO -, DPPH_ and ABTS radical scavenging activities (0.38 6 0.01, 1.24 6 0.03 and 0.17 6 0.01 mg/mL, respectively). The pooled fraction formed after Reverse phase-High-performance liquid chromatography (HPLC) (F 4-5 ) had the highest percentage inhibition of radical scavenging for DPPH_, and NO -(25.04 6 0.88, and 6.50 6 0.39%, respectively). LC-MS of F 4-5 revealed peptides exhibiting antioxidant activity were composed of His (H), Trp (W) and Tyr (Y) that known to enhance the scavenging activities of peptides. The F4 fraction suppressed lipopolysaccharide and rmIFN-g mediated increase in iNOS, IL-6 and TNF-a mRNA levels in RAW264.7 cells, suggesting the interference was mediated at the transcriptional level.
PRACTICAL APPLICATIONDefatted rice bran (DRB) is a good source of nutritional proteins. In this study, DRB protein was hydrolyzed using proteases and further fractionated by ultrafiltration. The results demonstrated that DRB hydrolysates and their fractions exhibited antioxidant and anti-inflammatory activities indicating these as a potential source of functional food and medicines.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.