Isolation and characterization of anti-inflammatory peptides derived from trypsin hydrolysis of microalgae protein (Synechococcussp. VDW)

Two novel ACE inhibitory peptides isolated from longan seeds: purification, inhibitory kinetics and mechanisms

“…Suttisuwan et al 22 noted that ultraltration can be employed to fractionate pepsin and pancreatin hydrolysates using a highperformance ultraltration cell (Pellicon XL Filter; Merck Millipore, Billerica, MA). In this study, three molecular weight cutoff (MWCO) membranes were sequentially employed (10, 5, and 3 kDa MWCO) to create four fractions (>5-10, 3-5, and <3 kDa).…”

The antioxidant potential of peptides obtained from the spotted babylon snail (Babylonia areolata) in treating human colon adenocarcinoma (Caco-2) cells

“…2.4.3 Nitric oxide (NO) radical scavenging assay. The nitric oxide radical scavenging activity was performed in accordance with the approach of Suttisuwan et al, 20 whereby nitric oxide radicals were produced using a sodium nitroprusside solution which generated the nitrite ions which could be evaluated through the Griess reaction. This process required that 25 mL of the various hydrolysates at differing dilutions be combined with 10 mM sodium nitroprusside in a phosphate buffer at a pH value of 7.2, before incubation for 150 minutes at room temperature.…”

Bioprospecting of microalgae metabolites against cytokine storm syndrome during COVID-19

“…Currently, there is growing research interest for microalgal BPs due to their potential anti-oxidant and anti-hypertensive abilities especially in blocking the hypertension-related angiotensin I-converting enzyme (ACE) [ 60 ]. The versatility of BPs extends to anti-inflammatory effects wherein for instance, Suttisuwan et al [ 61 ] identified 5 peptide sequences from the freshwater microalga Synechococcus sp . that were effective against LPS-stimulated iNOS and NO production, as well as reducing gene expression for COX-2, IL-6 and TNF-α [ 61 ].…”