The antioxidant potential of peptides obtained from the spotted babylon snail (<i>Babylonia areolata</i>) in treating human colon adenocarcinoma (Caco-2) cells

Petsantad, Putcha; Sangtanoo, Papassara; Srimongkol, Piroonporn; Saisavoey, Tanatorn; Reamtong, Onrapak; Chaitanawisuti, Nilnaj; Karnchanatat, Aphichart

doi:10.1039/d0ra03261a

Cited by 14 publications

(11 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that snail mucus from H. aspersa could inhibit the tyrosinase activity and melanin production on cell lines [11,14]. In other snails, the extracts from different parts showed an antioxidant activity and the presence of many bioactive compounds [38][39][40][41]. In this study, we showed that the snail mucus from both L. fulica and H. distincta also exhibited an anti-tyrosinase as well as antioxidant activity.…”

Section: Discussionsupporting

confidence: 52%

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Noothuan

Apitanyasai

Panha

et al. 2021

Preprint

View full text Add to dashboard Cite

Objective: Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical and cosmetic applications. In this study, we explored the protein pattern and compared the biological properties of the mucus secreted from the mantle collar and foot of two snail species, Lissachatina fulica and Hemiplecta distincta.Result: Protein profile showed a different pattern between the two species and between the two secretory parts. The mantle-specific protein bands were further characterized and among them was an antibacterial protein, achacin. Accordingly, the mucus from the mantle exhibited the higher antibacterial activity than that from the foot in both snail species. The mucus from H. distincta, first reported here, also showed antibacterial properties, but with a lower activity compared to that for L. fulica. Snail mucus also exhibited anti-tyrosinase activity and antioxidant activity but with no significant difference between the foot and mantle mucus. These results indicate some different protein compositions and biological activities of snail slime from the mantle and foot, which might be associated with their specific functions in the animal and are useful for medical applications.

show abstract

Section: Discussionsupporting

confidence: 52%

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Noothuan

Apitanyasai

Panha

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…PAAEs were performed by using ABTS radical cation decolorization activity ( Petsantad et al, 2020 ). Seven millimolar of ABTS (Sigma, United States) solution was mixed with 2.45 mM of potassium persulfate (Ajax Finechem, Australia) and incubated at room temperature for 12–16 h in darkness to create an ABTS cation radical.…”

Section: Methodsmentioning

confidence: 99%

“…PAAEs were carried out using DPPH radical-scavenging activity ( Petsantad et al, 2020 ; Chit-aree et al, 2021 ). The DPPH (Sigma, United States) radical solution (0.1 mM) was prepared in methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Tilapia peptides have been demonstrated to inhibit proliferation of adenocarcinoma cells (HeLa), hepatocellular carcinoma cells (HepG2), fibrosarcoma cells (HT1080), Cercopithecus aethiops kidney cells (COS-7), and human kidney cells (WS-1; Chang et al, 2011a ). Spotted babylon snail peptides prevent the development of adenocarcinoma colon cells (Caco-2; Petsantad et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

et al. 2022

View full text Add to dashboard Cite

Earthworms have long been used as traditional medicine. The purposes of this research were to create bioactive peptides from the unique Amynthas arenulus earthworm (PAAEs) and test their potentials on liver cancer bioprophylactic activity, antioxidant, oxidative stress protection, and immune cell activation. This earthworm had a high protein content ratio, at 55.39%. Besides, PM 35 is one out of 58 bacteria isolated from the earthworm carcasses that exhibited the highest protease and yield protein production which was chosen as the protease-producing bacteria to hydrolyze the protein. The genera were identified by 16S rRNA and 16S–23S rRNA comparison and confirmed as Bacillus velezensis PM 35. The response surface methodology was applied to optimize these hydrolysis parameters, i.e., the enzyme/substrate (E/S) concentration ratio [1%–3% (v/v)] and time (1–3 h) of the hydrolyzing earthworm’s proteins. The optimal hydrolyzing conditions were 3% (v/v) of E/S concentration ratio and 3 h of hydrolysis time, which found protein-hydrolysate yield (24.62%) and degree of hydrolysis (85.45%) as the highest. After being challenged in the gastrointestinal tract-resistant model, these PAAEs (MW <3 and 3–5 kDa) induced liver cancer cell (HepG2) death via apoptotic action modes (cell morphological change and DNA fragmentation). The PAAEs (MW <3 kDa) exhibited significant antioxidant activity via DPPH, ABTS, and FRAP with IC50 values of 0.94, 0.44, and 6.34 mg/ml, respectively. The PAAEs (MW < 3 kDa) were non-cytotoxic and protected the mouse fibroblast cells (L929) against oxidative stress. These PAAEs (MW < 3 kDa, 0.2 mg/ml) stimulated the B lymphocytes (122.3%), and T lymphocytes (126.7%) proliferation. This research suggests that PAAEs can be used in a variety of applications, especially in the food and pharmaceutical industries.

show abstract

“…The majority of the biologically active peptides identified to date can be found in the BIOPEP-UWM database [ 9 ] and include antimicrobial, dipeptidyl peptidase-IV (DPP-IV; EC 3.4.14.5), angiotensin-I-converting enzyme (ACE-I; EC 3.4.15.1) and prolyl endopeptidase (PEP; EC 3.4.21.26)-inhibitory peptides, as well as antioxidant peptides) [ 10 ]. Recently, protein hydrolysates were developed from the spotted Babylon snail Babylonia areolata using pepsin and pancreatin and the antioxidant activity of the resultant hydrolysate was assessed using the 2,2-Diphenyl-1-picrylhydrazl (DPPH) radical scavenging activity and 2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity assays as well as a cellular antioxidant assay [ 11 ]. Two antioxidant peptides with the amino acid sequences HTYHEVTKH and WPVLAYHFT were identified using MS and WPVLAYHFT had higher radical scavenging activities likely due to the hydrophobic nature of the amino acids within its structure.…”

Section: Introductionmentioning

confidence: 99%

Alternative Proteins as a Source of Bioactive Peptides: The Edible Snail and Generation of Hydrolysates Containing Peptides with Bioactive Potential for Use as Functional Foods

Hayes

Mora

2021

Foods

View full text Add to dashboard Cite

Members of the Phylum Mollusca include shellfish such as oysters and squid but also the edible garden snail known as Helix aspersa. This snail species is consumed as a delicacy in countries including France (where they are known as petit-gris), southern Spain (where they are known as Bobe), Nigeria, Greece, Portugal and Italy but is not a traditional food in many other countries. However, it is considered an excellent protein source with a balanced amino acid profile and an environmentally friendly, sustainable protein source. The aim of this work was to develop a different dietary form of snail protein by generating protein hydrolysate ingredients from the edible snail using enzyme technology. A second aim was to assess the bioactive peptide content and potential health benefits of these hydrolysates. H. aspersa hydrolysates were made using the enzyme Alcalase® and the nutritional profile of these hydrolysates was determined. In addition, the bioactive peptide content of developed hydrolysates was identified using mass spectrometry. The potential heart health benefits of developed snail hydrolysates were measured in vitro using the Angiotensin-I-converting Enzyme (ACE-1; EC 3.4.15.1) inhibition assay, and the ACE-1 inhibitory drug Captopril© was used as a positive control. The generated H. aspersa hydrolysates were found to inhibit ACE-1 by 95.60% (±0.011) when assayed at a concentration of 1 mg/mL (n = 9) compared to the positive control Captopril© which inhibited ACE-1 by 96.53% (±0.0156) when assayed at a concentration of 0.005 mg/mL (n = 3). A total of 113 unique peptide sequences were identified following MS analysis with peptides identified ranging from 628.35 Da (peptide GGGLVGGI—protein accession number sp|P54334|XKDO_BACSU) to 2343.14 Da (peptide GPAGVPGLPGAKGDHGFPGSSGRRGD—protein accession number sp|Q7SIB2|CO4A1_BOVIN) in size using the BIOPEP-UWM database.

show abstract

The antioxidant potential of peptides obtained from the spotted babylon snail (Babylonia areolata) in treating human colon adenocarcinoma (Caco-2) cells

Abstract:
The isolation and subsequent identification of the two novel antioxidant peptides, HTYHEVTKH, and WPVLAYHF from the spotted babylon snail was achieved. In the Caco-2 cell line, two synthetic peptides produced a dose-dependent response on antioxidant activity.

Cited by 14 publications

References 61 publications

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

Alternative Proteins as a Source of Bioactive Peptides: The Edible Snail and Generation of Hydrolysates Containing Peptides with Bioactive Potential for Use as Functional Foods

Contact Info

Product

Resources

About

The antioxidant potential of peptides obtained from the spotted babylon snail (Babylonia areolata) in treating human colon adenocarcinoma (Caco-2) cells

Abstract: The isolation and subsequent identification of the two novel antioxidant peptides, HTYHEVTKH, and WPVLAYHF from the spotted babylon snail was achieved. In the Caco-2 cell line, two synthetic peptides produced a dose-dependent response on antioxidant activity.

Cited by 14 publications

References 61 publications

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Snail Mucus From the Mantle and Foot of Two Land Snails, Lissachatina Fulica and Hemiplecta Distincta, Exhibits Different Protein Profile and Biological Activity

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

Alternative Proteins as a Source of Bioactive Peptides: The Edible Snail and Generation of Hydrolysates Containing Peptides with Bioactive Potential for Use as Functional Foods

Contact Info

Product

Resources

About

Abstract:
The isolation and subsequent identification of the two novel antioxidant peptides, HTYHEVTKH, and WPVLAYHF from the spotted babylon snail was achieved. In the Caco-2 cell line, two synthetic peptides produced a dose-dependent response on antioxidant activity.