The Galactic population components and their structural parameter values

Rong, Jianxiang; Buser, R.

doi:10.1007/bf02886237

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…Generally, artificial nucleases are classified on the basis of cleavage mechanism in free radical, hydrolytic and elimination cleavage agents. 55 For identification of the cleavage mechanism, in vitro experiments are performed by using activated model compounds of nucleic acids (e.g. p-nitrophenol phosphates).…”

Section: Introductionmentioning

confidence: 99%

PNA-based artificial nucleases as antisense and anti-miRNA oligonucleotide agents

Gaglione¹,

Milano²,

Moggio

et al. 2011

Mol. BioSyst.

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Because of its interesting chemical, physical and biological properties, Peptide Nucleic Acid (PNA) has attracted major attention in molecular biology, for diagnostics purposes and development of biosensors. PNAs have become candidates for gene therapeutic drugs in ANTISENSE (AO) strategy with favorable in vivo biochemical properties. Recently, antisense PNA oligonucleotides have been described in anti-miRNA approach (AMO). We propose PNA-based nucleases as AO and AMO agents. We report the design, synthesis and characterization of two kinds of artificial nucleases composed of a PEG-PNA-PEG domain conjugated to HGG·Cu (A) and DETA (B) as well known cleavage sites. Qualitative (MALDI-TOF) and quantitative (HTS) assays were planned to study nuclease activity of constructs A and B on RNA-3'-FAM target sequence. The results have highlighted the best performance of nuclease B and the relevance of the PEG spacer, in particular for conjugate A, in terms of efficiency of the cleavage, suggesting that conjugates A and B also act as potential antisense and anti-miRNA agents.

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Section: Introductionmentioning

confidence: 99%

PNA-based artificial nucleases as antisense and anti-miRNA oligonucleotide agents

Gaglione¹,

Milano²,

Moggio

et al. 2011

Mol. BioSyst.

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“…In particular, lanthanides are known as excellent catalysts of DNA cleavage [3][4][5]. They hydrolyze phosphodiester linkages in simple phosphate esters, oligonucleotides, RNA and single stranded DNA at reasonable rates under physiological conditions [3,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Cleavage of double stranded plasmid DNA by lanthanide complexes

Rittich

Španová

Falk

et al. 2004

Journal of Chromatography B

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Isomeric Effect on the anticancer Behavior of two Zinc (II) complexes based on 3,5‐bis(1‐imidazoly) pyridine: Experimental and Theoretical Approach

Zhu

Song

Liu³

et al. 2019

Applied Organom Chemis

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Two isomeric Zinc (II) complexes constructed by 3,5-bis(1-imidazoly) pyridine has been synthesized and characterized by single crystal X-ray diffraction, elemental analyses and infrared spectroscopy. The binding mode and ability of complex 1-2 with CT-DNA were studied by UV and fluorescence spectra. The intrinsic binding constant K b (K b1 = 2.305 × 10 4 M −1 , K b2 = 3.095 × 10 4 M −1 ) and the observed association constant K obs (K obs1 = 1.523*10 6 M −1 , K obs2 = 2.057*10 6 M −1 ) indicated that the insertion ability of complex 2 with CT-DNA is stronger than complex 1. Gel electrophoresis showed that complexes have a good ability to hydrolyze cleavage pBR322 plasmid DNA. The cytotoxicity and apoptosis studies showed that complexes exhibited excellent cytotoxic activity against HeLa cells, especially complex 2 had better growth inhibition than Cisplatin. Molecular docking study simulated the binding model of complexes with DNA (PDB:4av1), showing an imidazole plane of complex 2 can be inserted into a DNA base pair in relative parallel. Both complexes can be used as potential anticancer agents.

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The Galactic population components and their structural parameter values

Cited by 5 publications

References 2 publications

PNA-based artificial nucleases as antisense and anti-miRNA oligonucleotide agents

PNA-based artificial nucleases as antisense and anti-miRNA oligonucleotide agents

Cleavage of double stranded plasmid DNA by lanthanide complexes

Isomeric Effect on the anticancer Behavior of two Zinc (II) complexes based on 3,5‐bis(1‐imidazoly) pyridine: Experimental and Theoretical Approach

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