Cleavage of double stranded plasmid DNA by lanthanide complexes

Rittich, Bohuslav; Španová, Alena; Falk, Martin; Beneš, Milan J.; Hrubý, Martin

doi:10.1016/j.jchromb.2003.09.011

Cited by 25 publications

(17 citation statements)

References 23 publications

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“…Researchers have considered the impacts of low-dose radiation, given as a single dose or fractionated or prolonged over time, with regard to altering cancer progression or resolution as well as their impacts on the systemic immune system [23] .It was found that low-dose radiation increases cellular antioxidant activity; facilitates DNA damage repair; reduces malignant transformation and mutagenesis, and stimulates immune surveillance [24]. The cell nucleus and DNA are susceptible to several stressors[25, 26,27,28] and can be greatly harmed with relatively low doses of ionizing radiation [29,30]. As adverse effects of ionizing radiation on (cancer) cells are mainly mediated by fragmentation of nuclear chromatin via inserting double strand breaks (DSBs) into the DNA molecule [31].…”

Section: Discussionmentioning

confidence: 99%

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Mohammad¹,

Ahmed²,

Kandil³

et al. 2019

IJASTR

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Guided treatments with nanoparticles and low doses of radiation are a new approach in cancer therapy. This study evaluated the ability of Chitosan-Gallium nanoparticles (Ch.GaNPs) and low dose ofγ-radiation to suppressoxidative stressof female mice bearing solid Ehrlich carcinoma (EC)in the neck region. Begins from 7 th day of EC cells inoculation Ch.GaNPs(0.5 mg/kg body weight) were orally administered via gavages for a period of 15 consecutive days and Whole-body γ -irradiation was carried out at a single dose of 0.25Gy/twice a week for 2 weeks. Tumor size was monitored. Oxidative stress markers were assessed, histopathological changes in tumor and liver tissues were studied. In addition, the angiogenic markers concentrations were evaluated.Treatment of EC-bearing mice with Ch.GaNPs and/or low doseof γ -radiation exposure significantly reduced tumor volume, increased lipid peroxidation and decreased glutathione content as well as glutathione peroxidase activity intumor and liver tissues. Moreover, the dual treatment significantly reducedserumVEGF, PDGF and TNF-α levels, and significantly elevated Cas-3 activity. These results were well appreciated with the histopathologicalfindings in the tumor and liver tissue. In conclusion, the combined treatment of Ch.GaNPs and low dose ofγ-radiation have a role in suppressing oxidative stress of female mice bearing solid Ehrlich carcinoma .

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Section: Discussionmentioning

confidence: 99%

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Mohammad¹,

Ahmed²,

Kandil³

et al. 2019

IJASTR

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“…The ability to precisely and rapidly monitor DNA double-strand break (DSBs) induction and repair undermines numerous fields of biological, medical and space research (reviewed e.g., in [1][2][3][4]). DNA double-strand breaks (DSBs) are permanently introduced into the DNA molecule by ionizing radiation, radiomimetic chemicals and vital cell processes [5][6][7]. Simultaneously cutting both DNA strands, DSBs represent the most serious type of DNA lesions [8], which accumulation, if left unrepaired, fuels ageing [9], neurodegeneration [10], infertility [11], and other health consequences.…”

Section: Introductionmentioning

confidence: 99%

DeepFoci: Deep Learning-Based Algorithm for Fast Automatic Analysis of DNA Double Strand Break Ionizing Radiation-Induced Foci

Vičar

Gumulec

Kolář

et al. 2020

Preprint

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DNA double-strand breaks, marked by Ionizing Radiation-Induced (Repair) Foci (IRIF), are the most serious DNA lesions, dangerous to human health. IRIF quantification based on confocal microscopy represents the most sensitive and gold standard method in radiation biodosimetry and allows research of DSB induction and repair at the molecular and a single cell level. In this study, we introduce DeepFoci - a deep learning-based fully-automatic method for IRIF counting and its morphometric analysis. DeepFoci is designed to work with 3D multichannel data (trained for 53BP1 and γH2AX) and uses U-Net for the nucleus segmentation and IRIF detection, together with maximally stable extremal region-based IRIF segmentation.The proposed method was trained and tested on challenging datasets consisting of mixtures of non-irradiated and irradiated cells of different types and IRIF characteristics - permanent cell lines (NHDF, U-87) and cell primary cultures prepared from tumors and adjacent normal tissues of head and neck cancer patients. The cells were dosed with 1-4 Gy gamma-rays and fixed at multiple (0-24 h) post-irradiation times. Upon all circumstances, DeepFoci was able to quantify the number of IRIF foci with the highest accuracy among current advanced algorithms. Moreover, while the detection error of DeepFoci remained comparable to the variability between two experienced experts, the software kept its sensitivity and fidelity across dramatically different IRIF counts per nucleus. In addition, information was extracted on IRIF 3D morphometric features and repair protein colocalization within IRIFs. This allowed multiparameter IRIF categorization, thereby refining the analysis of DSB repair processes and classification of patient tumors with a potential to identify specific cell subclones.The developed software improves IRIF quantification for various practical applications (radiotherapy monitoring, biodosimetry, etc.) and opens the door to an advanced DSB focus analysis and, in turn, a better understanding of (radiation) DNA damaging and repair.HighlightsNew method for DSB repair focus (IRIF) detection and multi-parameter analysisTrainable deep learning-based methodFully automated analysis of multichannel 3D datasetsTrained and tested on extremely challenging datasets (tumor primary cultures)Comparable to an expert analysis and superb to available methodsGraphical Abstract

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“…There has been an increasing demand for the investigation of DNA-targeting molecules and the mechanism of their activity towards DNA, and special attention has been paid to the intercalating agents as a class of anti-tumor compounds (31,32).…”

Section: Introductionmentioning

confidence: 99%

DNA breakage induced by piceatannol and copper(II): Mechanism and anticancer properties

Yang

Dong

et al. 2012

Oncology Letters

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Abstract. Piceatannol (3,3',4,5'-tetrahydroxy-trans-stilbene; Pice), found in a variety of plant sources including grapes, red wine, peanuts and rhubarb, is known as a metabolite and analog of Resveratrol (3,5,4'-trihydroxy-trans-stilbene; Res) and has higher bioactivity than Res. To explore the mechanism of DNA damage induced by Pice in the presence of copper (Cu) (II), gel electrophoresis, UV-visible spectroscopy, fluorescence spectroscopy and Fourier transform infrared spectroscopy were used. The results of gel electrophoresis demonstrated that the hydroxyl radical played a critical role in DNA cleavage. Spectroscopy confirmed that the mechanism of DNA cleavage induced by Pice-Cu(II) involves the Haber Weiss and Fenton reactions. Pice chelates with Cu(II) as a bidentate ligand, and the Pice-Cu(II) complex undergoes intramolecular electron transfer to form the semiquinone radical anion and Cu(I), which may be reoxidated by O 2 to form Cu(II) with hydroxyl radical generation. In brief, the formation of the hydroxyl radical and the Cu(II)/Cu(I) redox cycle play a key role in inducing DNA damage. In this process, Pice demonstrated pro-oxidant properties. Oxidative product(s) of Pice, semiquinone, was formed and Cu(I) was reoxidized to Cu(II). The redox cycling of copper generated reactive oxygen species, which induced DNA cleavage, the hallmark of cell apoptosis. The mechanism of DNA breakage induced by Pice-Cu(II) may be a significant pathway through which cancer cells are killed.

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Cleavage of double stranded plasmid DNA by lanthanide complexes

Cited by 25 publications

References 23 publications

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

DeepFoci: Deep Learning-Based Algorithm for Fast Automatic Analysis of DNA Double Strand Break Ionizing Radiation-Induced Foci

DNA breakage induced by piceatannol and copper(II): Mechanism and anticancer properties

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