Mesenchymal stem cells have therapeutic properties that are related to their potentials for trans-differentiation, immunomodulation, anti-inflammatory, inhibitory effect on tumor proliferation, and induction of apoptosis. This study was performed to analyze the role of mesenchymal stem cells as an alternative for cellular signaling growth factors involved in the pathogenesis of leukemogenesis in rats. Treatment of rats with 7,12-dimethyl benz [a] anthracene induced leukemogenesis appeared as a significant decrease in hematological parameters with concomitant significant increase in bone marrow oxidative and inflammatory indices (transforming growth factor beta and interleukin-6) in comparison with normal groups. On the contrary, Western immunoblotting showed a significant increase in the signaling growth factors: PI3K, AKT, mTOR proteins and a significant decrease in PTEN in 7,12-dimethyl benz [a] anthracene-treated group. In addition, a significant increase in the transcript levels of B cell lymphoma-2 protein gene in the 7,12-dimethyl benz [a] anthracene group, while that of C-X-C motif chemokine receptor-4 and B cell lymphoma-2 protein associated x-protein were significantly downregulated compared to controls. Meanwhile, therapeutic mesenchymal stem cells treatment predict a significant improvement versus 7,12-dimethyl benz [a] anthracene group through the modulation of growth factors that confront bone marrow dysplasia. In the same direction treatment of 7,12-dimethyl benz [a] anthracene group with mesenchymal stem cells, it induced apoptosis and increased the homing efficacy to bone marrow. In conclusion, mesenchymal stem cells improve hematopoiesis and alleviate inflammation, and modulated PI3K/AKT signaling pathway contributed to experimental leukemogenesis.
Omega-3 essential fatty acids (ω-3 FATs); found in the highest concentrations in fish oil, claim a plethora of health benefits. The present study aims to evaluate the biological effects of ω-3 FATs supplementation against Ehrlich carcinoma (EC) induced inflammation, oxidative stress, biochemical and histopathological alterations in the liver tissue of albino mouse. ω-3 FATs were orally administered via gavage to mice for a period of 30 consecutive days at a dose of 300 mg/kg body weight. On the 7th day of ω-3 FATs administration, female mice were subcutaneously injected with 0.2 ml of Ehrlich ascite carcinoma for solid tumor induction. The present study revealed that, subcutaneous injection of Ehrlich solid tumor led to hepatic oxidative stress (as significant increase in lipid peroxidation (thiobarbituric acid reactive species, TBARS), concomitant with a significant decrease in glutathione and antioxidant enzymes), systemic inflammation (significant increases in C-reactive protein, tumor necrosis factoralpha and leukocyte counts) and biochemical alterations (as increase in liver function enzymes)). While in the tumor tissue, significant increase in tumor TBARS content and non significant changes in glutathione and antioxidant enzymes were observed. Histopathological studies showed that EC cells metastasis caused fatty degeneration, enlargement of liver cells nuclei and presence of necrosis. Pretreatment of animals with ω-3 FATs significantly reduced tumor size and markedly improved most of the biochemical parameters associated with the inoculation of EC. It could be concluded that ω-3 FATs administrated to mice, reduce tumor size, inhibit systemic inflammation, improving liver function profile, modulating lipid peroxidation and augmenting antioxidant defense system in EC bearing mice.
Trigonella foenum-graceum extract either alone or combined with selenium nanoparticles exhibited antitumor effect. Ehrlich ascite carcinoma (EAC) cell line and four groups of female mice were used. Solid Ehrlich carcinoma (EC) was induced by inoculation of 2.5x106 cells in left thighs of each animal. Mice were gavage orally by 2.5 µg/0.1 ml of Trigonella foenum-graceum extract either alone or combined with selenium nanoparticles daily during one month. Tumor size, serum tumor markers (TNF-α, IFN- γ, Granzyme-B and Caspase-3) were measured. Oxidative stress, antioxidant markers, Histopathological, apoptotic and necrotic examinations were determined in tumor tissues. Tumor size of experimental groups represents reduction. Caspase-3 as well as Granzyme-B activities were significantly elevated along with diminishing tumor size while, TNF-α and IFN-γ levels were decreased in serum. Meanwhile, oxidative stress marker (MDA) was significantly decreased in tumor tissue. The tumor GSH content and CAT activity were increased. Histopathological, apoptotic and necrotic examinations were context with previous conclusion. It could be concluded that Trigonella foenum-graceum extract either alone or combined with SeNPs exhibited antitumor effect which is reflected by a inhibition in tumor size, a decrease of serum TNF-α and IFN-γ, an increase in serum caspase-3 and Granzyme-B, reduction in tumor MDA and an increase in tumor GSH and CAT which cause regulate tumor regression.
5-Fluorouracil (5-FU) as anticancer drug has many side effects. Site-specific delivery of 5-FU would reduce the systemic side effects and provide effective and safe therapy. Chitosan nanoparticles (CNPs) are used in drug delivery systems. In the present study the effects of 5-fluorouracil chitosan nanoparticles (5FUCNPs) on decreasing the oxidative stress were investigating within a model of female mice bearing solid Ehrlich carcinoma (EC). 7 days After solid tumor induction, 5-FUCNPs were administrated by gavages (0.5 mg/kg body weight) to mice for 15 days. Tumor size was monitored; oxidative stress markers were assessed. In addition, the angiogenic markers concentrations were evaluated. In vitro, 5FUCNPs showed high cytotoxic effect on EC tumor cells. Gavages of EC-bearing mice with 5FUCNPs significantly reduced tumor size, increased MDA level, decreased GSH level and recorded great destruction in tumor tissues. Meanwhile, in liver tissue MDA level significantly decreased and GSH level increased. On the other hand, a significant decrease in the levels of angiogenic markers were recorded. In conclusion: CNPs as a drug carrier for 5-FU have a role in reducing tumor growth and may represent a novel class of anticancer drug.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.