The Fabrication of a Highly Sensitive Nano Green Carbon Paste Electrode Modified with Yttrium Doped Manganese Oxide (Mn₂O₃/Y₂O₃) for Electrochemical Determination of Marbofloxacin and Its Residues in Bovine Meat and Milk Samples

Abstract

In the present work, a novel sensitive electrochemical carbon paste electrode chemically modified with yttrium doped manganese oxide Mn 2 O 3 /Y 2 O 3 nanostructures was assigned for determination of marbofloxacin (MRB) using square wave voltammetry (SWV) method. MRB has a broad spectrum of bactericidal activity for the treatment of urinary, respiratory and dermatological diseases in bovines and their retention in animal meats and milk leads to adverse side effects for the consumer. Thus a rapid estimation of minor concentrations of MRB has exerted a great concern to ensure food safety. XRD, EDX, Raman spectroscopy, SEM, and TEM techniques were employed to characterize the samples. The electrochemical oxidation behavior of MRB shows irreversible anodic peak at 1.10 V vs Ag/AgCl, in Britton-Robinson buffer (BR) at pH 5.0. The relationship was rectilinear over the range 10 × 10 −9 -1.0 × 10 −4 M between the peak current and its related concentration with a minimum detection limit of 2.4 × 10 −9 M. The developed method was green chemistry challenges and successfully applied to assay the drug in its dosage form, bovine meat and milk samples with a good recovery lies between 94.56% and 105.33% with relative standard deviation less than 10%.

Voltammetric Determination of Marbofloxacin at Carbon Paste Sensor Integrated with Copper Oxide Nanoparticles

“…Based on the results illustrated in the effect of pH, and scan rate studies (Figure 2&3) with the aid of molecular orbital calculations (S1 and S2), the irreversible electrochemical oxidation of marbofloxacin undergoes through the oxidation of the amino group in the piperazinyl ring accompanied with the transferring of two electrons and proton exchange (Scheme 1). This suggested electrooxidation mechanism disagree with that reported elsewhere applying Mn 2 O 3 /Y 2 O 3 /CPEs electrodes in which the marbofloxacin molecules was oxidized through attacking of the piperazinyl ring via the hydroxyl radicals generated by the electrode, which in turn oxidized by loss of 2H to produce a keto‐derivative of the piperazinyl ring [22].…”

“…Prolonged storage resulted in deterioration of the sensor performance by about 5.0 %. Compared with the reported MRB sensors [21, 22], the present work showed improved performance as indicated by the lower LOD and LOQ values with prolonged operational life time (S3).…”

“…Regarding MRB quantification, a glassy carbon electrode modified with Nafion layer has been successfully applied for MRB detection in animal serum samples [21]. More recently, carbon paste electrodes incorporated with mixed yttrium‐manganese oxides (Mn 2 O 3 /Y 2 O 3 /CPEs) were utilized for the square wave voltammetric determination of marbofloxacin in bovine meat and milk samples [22].…”

Modulation of the Morphological Architecture of Mn2O3 Nanoparticles to MnCoO Nanoflakes by Loading Co3+ Via a Co-Precipitation Approach for Mosquitocidal Development

“…As described in Figure 2 a, the Mn 2 O 3 spectrum composed of Mn and O elements of weight percent 74.22 and 25.78 wt.%, respectively. In Figure 2 b, the EDAX spectrum proved that the cobalt atoms were successfully incorporated into the host Mn 2 O 3 (17.12 wt.%), resulting in the production of MnCoO nanocomposite [ 32 , 33 ].…”

Metal–Organic Framework-Based Composites for the Detection and Monitoring of Pharmaceutical Compounds in Biological and Environmental Matrices

“…An irreversible electrochemical oxidation pattern was demonstrated by MRB at 1.10 V vs Ag/AgCl. The minimal DL was 2.4 × 10 −9 M. The method was applicable for MRB detection in milk, meat, and bovine samples (Abdel-Atty et al, 2020 ). A similar method was demonstrated by Min et al by GCE modification with nafion for accurate detection of MBF in serum vs Ag/AgCl at a potential of + 0.15 V with reported DL of 5 × 10 −9 mol L −1( Min et al, 2009 ).…”