The Estimation of Catecholamines in Urine by a Chemical Method

Weil‐Malherbe, H.; Bone, A. D.

doi:10.1136/jcp.10.2.138

Cited by 60 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Trihydroxyindole has characteristic fluorescent properties, and detection of the fluorescence provided the basis for several methods that proved adequate to measure catecholamine concentrations in tissues [115]. Another type of fluorimetric assay was based on the condensation of oxidized catecholamines with ethylenediamine [116, 117], and this enabled separate measurement of NE and EPI in urine [118] and plasma [119]. …”

Section: Catecholamine Reactivity To Mental Stressmentioning

confidence: 99%

Sympathoneural and Adrenomedullary Responses to Mental Stress

Carter

Goldstein

2014

Comprehensive Physiology

View full text Add to dashboard Cite

This concept-based review provides historical perspectives and updates about sympathetic noradrenergic and sympathetic adrenergic responses to mental stress. The topic of this review has incited perennial debate, because of disagreements over definitions, controversial inferences, and limited availability of relevant measurement tools. The discussion begins appropriately with Cannon's "homeostasis" and his pioneering work in the area. This is followed by mental stress as a scientific idea and the relatively new notions of allostasis and allostatic load. Experimental models of mental stress in rodents and humans are discussed, with particular attention to ethical constraints in humans. Sections follow on sympathoneural to mental stress, reactivity of catecholamine systems, clinical pathophysiologic states, and the cardiovascular reactivity hypothesis. Future advancement of the field will require integrative approaches and coordinated efforts between physiologists and psychologists on this interdisciplinary topic.

show abstract

Section: Catecholamine Reactivity To Mental Stressmentioning

confidence: 99%

Sympathoneural and Adrenomedullary Responses to Mental Stress

Carter

Goldstein

2014

Comprehensive Physiology

View full text Add to dashboard Cite

show abstract

“…Adrenaline and noradrenaline were estimated fluorimetrically by the method of Weil-Malherbe and Bone. 5 The results showed that there was no obvious or adrenaline and noradrenaline. However, this method is not readily applicable to studies of this kind.…”

Section: Stress In Operating Theatre Personnelmentioning

confidence: 95%

Stress in operating theatre personnel

Mehta

Burton

1977

Anaesthesia

View full text Add to dashboard Cite

Correspondence hyperinsulinism is considered.'S2 Furthermore, many cases have been described from a swgical ~i e w p o i n t .~.~The choice of anaesthetic technique for these patients is of little significance. We have used both halothane and neuroleptanalgesia without difficulty. Provided that blood sugar estimations are frequent and appropriate quantities of dextrose are administered intravenously preoperatively and during surgery, severe hypoglycaemia should neither occur, nor should the effects of anaesthetic agents cause confusion. Stress in operating theatre personnel Department of Anesthesiology, R. MILLER Mount SinaiIn recent years attention has been focussed on the health risk run by operating theatre personnel. Rosenberg and Kirves' in the Finnish survey suggested that excessive work loads rather than anaesthetic gases are the major cause of obstetrical disturbances among nurses working in operating rooms. Smitha pointed out that we should not necessarily seek a single cause for these alleged potential hazards of being an anaesthetist. Contributory causes could include occupational stress arising from the incidence of uncertainty and anticipation exacerbated by irregular routine, fatigue and emotional factors. This study was undertaken to establish whether or not working in an operating theatre environment led to increased stress as evidenced by altered physiological and biochemical responses and to compare some of the findings with those obtained in nurses working in wards.Two series of experiments were undertaken, the first on two separate groups of workers. The first group was composed of nine male and six female volunteers, ranging in age from 24 to 60 years, who normally worked in the operating theatre as doctors, nurses and technicians. The second group consisted of six male and nine female nurses from the wards, their ages ranging from 26 to 57 years.All subjects were in normal health and were not taking any drugs, and were studied between the hours of 9.00 a.m. and 12.00 noon; there was no systematic difference in the time of day during which different groups were investigated. The measurements of pulse rate and blood pressure were taken at 9.00 a.m. and again at 12.00 noon. Blood pressure was taken by auscultatory method. Blood samples for cortisol estimation were also withdrawn at 9.00 a.m. and 12.00 noon. The measurement of plasma cortisol was made by the method of Mattingl~.~ In the second series, the urinary output of adrenaline and noradrenaline was estimated in six healthy male volunteers, ranging in age from 26 to 48 years, comprising of three anaesthetists, two surgeons and one theatre technician. The urine samples were collected in two periods from 9.00a.m.to12.00noonand2.00p.m.to5.00p.m.,on operating days and non-operating days, thus using each subject as his own control. The details in the experimental studies were strictly standardised, including food and fluid intake and urine collections.The volume of urine was measured during each period. Catecholamines were extracted from the a...

show abstract

“…The combined and individual concentrations of noradrenaline and adrenaline were estimated in 24-hour collections 411 of urine by a fluorometric method based on that of Weil-Malherbe and Bone (1957). 4-hydroxy-3-methoxymandelic acid (HMMA, formerly VMA) was determined by the spectrophotometric method of Pisano, Crout, and Abraham (1962).…”

Section: Methodsmentioning

confidence: 99%